Inhibition of fibrin-induced neurogenic pulmonary edema by previous unilateral left-vagotomy correlates with increased levels of brain nitric oxide synthase in the nucleus tractus solitarii of rats

2002 ◽  
Vol 102 (1-2) ◽  
pp. 1-7 ◽  
Author(s):  
Guo Gang Feng ◽  
Kimitoshi Nishiwaki ◽  
Hiroko Kondo ◽  
Yasuhiro Shimada ◽  
Naohisa Ishikawa
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Pulmonary Edema ◽  
Nucleus Tractus Solitarii ◽  
Neurogenic Pulmonary Edema ◽  
Oxide Synthase ◽  
Brain Nitric Oxide

scholarly journals Expression of rat brain nitric oxide synthase in baculovirus-infected insect cells and characterization of the purified enzyme

1994 ◽  
Vol 304 (3) ◽  
pp. 683-686 ◽  
Author(s):  
C Harteneck ◽  
P Klatt ◽  
K Schmidt ◽  
B Mayer
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Brain ◽  
Insect Cells ◽  
Specific Activity ◽  
Apparent Homogeneity ◽  
Infected Insect ◽  
High Level ◽  
Oxide Synthase ◽  
Brain Nitric Oxide

Rat brain nitric oxide synthase was expressed to a high level in baculovirus-infected insect cells and purified to apparent homogeneity by affinity chromatography. The enzyme had a specific activity of approximately 1 mumol of citrulline.min-1.mg of protein-1 and contained 0.93, 0.45, 0.18 and 0.23 mol of haem, (6R)-5,6,7,8-tetrahydro-L-biopterin (H4biopterin), FAD and FMN per mol of subunit respectively.

PGE2, via EP3 receptors, regulates brain nitric oxide synthase in the perinatal period

1998 ◽  
Vol 275 (6) ◽  
pp. R1812-R1821 ◽  
Author(s):  
Isabelle Dumont ◽  
Krishna G. Peri ◽  
Pierre Hardy ◽  
Xin Hou ◽  
Ana Katherine Martinez-Bermudez ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Perinatal Period ◽  
Adult Brain ◽  
Newborn Rats ◽  
Cox Inhibitor ◽  
Cox 2 ◽  
Oxide Synthase ◽  
Brain Nitric Oxide ◽  
Nnos Expression

We tested the hypothesis that high prostaglandin levels during the perinatal period might regulate brain nitric oxide synthase (nNOS) expression. nNOS and cyclooxygenase (COX)-2 mRNAs were higher in brain cortex and the periventricular area of newborn rats and pigs compared with adult brain. Nitric oxide synthase activity was also 2.5- to 4-fold higher in newborn than in adult brain. Administration of nonselective COX inhibitor ibuprofen or COX-2 inhibitor nimesulide every 8 h for 24 h to newborn rats and pigs reduced prostaglandin levels and caused comparable reductions in nNOS mRNA, protein, and activity to levels of adults; COX inhibitor-induced changes were prevented by cotreatment with PGE2 analog, 16,16-dimethyl-PGE2, and agonist for the EP3 receptor of PGE2, sulprostone, but not by PGI2 analog carbaprostacyclin, PGD2, EP1 receptor agonist 17-phenyl trinor-PGE2, and EP2 agonist butaprost. Concordant observations were made in vitro and revealed that nNOS expression (detected by NADPH diaphorase reactivity) mostly present in neurons of the deeper cortical layers was reduced by COX inhibitor, and this effect was prevented by EP3agonist. In conclusion, high levels of PGE2 in neonatal brain contribute to the increased expression of nNOS by acting on EP3 receptors; this positive interaction between PGE2 and nNOS might be required physiologically for normal brain development.

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