scholarly journals P127 Long-term outcomes of cohort segregation on the prevalence of a transmissible strain of Pseudomonas aeruginosa at an adult cystic fibrosis centre

2019 ◽  
Vol 18 ◽  
pp. S93
Author(s):  
J. Heaton ◽  
F. Frost ◽  
V. Yioe ◽  
A. Abdul ◽  
D. Nazareth ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Long Term Outcomes ◽  
Cystic Fibrosis Centre

How and why to monitor Pseudomonas aeruginosa infections in the long term at a cystic fibrosis centre

2016 ◽  
Vol 92 (1) ◽  
pp. 54-60 ◽  
Author(s):  
L. Kalferstova ◽  
K. Vilimovska Dedeckova ◽  
M. Antuskova ◽  
O. Melter ◽  
P. Drevinek
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Cystic Fibrosis Centre

scholarly journals Clinical significance of Pseudomonas aeruginosa 2-alkyl-4-quinolone quorum-sensing signal molecules for long-term outcomes in adults with cystic fibrosis

2019 ◽  
Vol 68 (12) ◽  
pp. 1823-1828 ◽  
Author(s):  
Karmel Webb ◽  
Andrew Fogarty ◽  
David A. Barrett ◽  
Edward F. Nash ◽  
Joanna L. Whitehouse ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Clinical Significance ◽  
Signal Molecules ◽  
Long Term Outcomes

scholarly journals Pseudomonas aeruginosa PA80 is a cystic fibrosis isolate deficient in RhlRI quorum sensing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Syed A. K. Shifat Ahmed ◽  
Michelle Rudden ◽  
Sabrina M. Elias ◽  
Thomas J. Smyth ◽  
Roger Marchant ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Virulence Factors ◽  
Genomic Islands ◽  
Clinical Strain ◽  
Whole Genome ◽  
Synonymous Mutations ◽  
Wide Range

AbstractPseudomonas aeruginosa uses quorum sensing (QS) to modulate the expression of several virulence factors that enable it to establish severe infections. The QS system in P. aeruginosa is complex, intricate and is dominated by two main N-acyl-homoserine lactone circuits, LasRI and RhlRI. These two QS systems work in a hierarchical fashion with LasRI at the top, directly regulating RhlRI. Together these QS circuits regulate several virulence associated genes, metabolites, and enzymes in P. aeruginosa. Paradoxically, LasR mutants are frequently isolated from chronic P. aeruginosa infections, typically among cystic fibrosis (CF) patients. This suggests P. aeruginosa can undergo significant evolutionary pathoadaptation to persist in long term chronic infections. In contrast, mutations in the RhlRI system are less common. Here, we have isolated a clinical strain of P. aeruginosa from a CF patient that has deleted the transcriptional regulator RhlR entirely. Whole genome sequencing shows the rhlR locus is deleted in PA80 alongside a few non-synonymous mutations in virulence factors including protease lasA and rhamnolipid rhlA, rhlB, rhlC. Importantly we did not observe any mutations in the LasRI QS system. PA80 does not appear to have an accumulation of mutations typically associated with several hallmark pathoadaptive genes (i.e., mexT, mucA, algR, rpoN, exsS, ampR). Whole genome comparisons show that P. aeruginosa strain PA80 is closely related to the hypervirulent Liverpool epidemic strain (LES) LESB58. PA80 also contains several genomic islands (GI’s) encoding virulence and/or resistance determinants homologous to LESB58. To further understand the effect of these mutations in PA80 QS regulatory and virulence associated genes, we compared transcriptional expression of genes and phenotypic effects with isogenic mutants in the genetic reference strain PAO1. In PAO1, we show that deletion of rhlR has a much more significant impact on the expression of a wide range of virulence associated factors rather than deletion of lasR. In PA80, no QS regulatory genes were expressed, which we attribute to the inactivation of the RhlRI QS system by deletion of rhlR and mutation of rhlI. This study demonstrates that inactivation of the LasRI system does not impact RhlRI regulated virulence factors. PA80 has bypassed the common pathoadaptive mutations observed in LasR by targeting the RhlRI system. This suggests that RhlRI is a significant target for the long-term persistence of P. aeruginosa in chronic CF patients. This raises important questions in targeting QS systems for therapeutic interventions.

scholarly journals Poor recovery from cystic fibrosis pulmonary exacerbations is associated with poor long-term outcomes

2017 ◽  
Vol 52 (10) ◽  
pp. 1268-1275 ◽  
Author(s):  
Don B. Sanders ◽  
Qianqian Zhao ◽  
Zhanhai Li ◽  
Philip M. Farrell
Keyword(s):  
Cystic Fibrosis ◽  
Long Term Outcomes ◽  
Poor Recovery ◽  
Pulmonary Exacerbations

scholarly journals Lytic activity by temperate phages of Pseudomonas aeruginosa in long-term cystic fibrosis chronic lung infections

2014 ◽  
Vol 9 (6) ◽  
pp. 1391-1398 ◽  
Author(s):  
Chloe E James ◽  
Emily V Davies ◽  
Joanne L Fothergill ◽  
Martin J Walshaw ◽  
Colin M Beale ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Lytic Activity ◽  
Lung Infections

scholarly journals RNASeq Based Transcriptional Profiling of Pseudomonas aeruginosa PA14 after Short- and Long-Term Anoxic Cultivation in Synthetic Cystic Fibrosis Sputum Medium

PLoS ONE ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. e0147811 ◽  
Author(s):  
Muralidhar Tata ◽  
Michael T. Wolfinger ◽  
Fabian Amman ◽  
Nicole Roschanski ◽  
Andreas Dötsch ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Transcriptional Profiling ◽  
Cystic Fibrosis Sputum ◽  
Short And Long Term
Sign in / Sign up

Export Citation Format

Share Document