High serum concentration of estradiol may be a risk factor of prostate volume

The goal of this study was to investigate the kinetics of NIH 3T3 fibroblast cell adhesion and their actin cytoskeleton organization on different geometry electrospun polycaprolactone nanofibrous scaffolds. Cell adhesion kinetics was measured by MTS assay. Cells on beaded scaffolds, which are not uniform fibrous scaffolds, showed the lowest adhesion rate and the smallest cytoskeleoton organization in all experiments. For uniform fiber scaffolds, cell adhesion rate was a function of scaffold specific surface area (SSA). Cell adhesion rate increased with increasing SSA when scaffold SSA was higher than 7.13 μm−1. Cell adhesion in high serum concentration culture medium was higher than in low serum concentration medium for all scaffolds. Results indicate that from 0–5% serum concentrations, cell adhesion at 4 or 8 hours were not significantly different between different geometry scaffolds. F-actin maximum projected area was quantified to indicate mature cell adhesion. Results indicate higher area for cells cultured in high serum concentration medium on 18.79 μm−1 (SSA) scaffolds, and had higher values when cultured for 8 hrs compared to 24 hrs. 10% serum concentration and 18.79 μm−1 (SSA) scaffolds are considered to be an optimum combination for enhanced cell adhesion.

Download Full-text

A High Serum Concentration of Azlocillin Affects Routine Biochemical Analyses

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456329303000319 ◽

1993 ◽

Vol 30 (3) ◽

pp. 329-330

Author(s):

A Gunneberg ◽

M G Gillett ◽

D J Goldie

Keyword(s):

Serum Concentration ◽

High Serum ◽

High Serum Concentration ◽

Biochemical Analyses

Download Full-text

Transfection of cultured myoblasts in high serum concentration with DODAC:DOPE liposomes

Gene Therapy ◽

10.1038/sj.gt.3300729 ◽

1998 ◽

Vol 5 (10) ◽

pp. 1306-1313 ◽

Cited By ~ 33

Author(s):

L Vitiello ◽

K Bockhold ◽

PB Joshi ◽

RG Worton

Keyword(s):

Serum Concentration ◽

High Serum ◽

High Serum Concentration

Download Full-text

Atopy as a risk factor for subclinical hypothyroidism development in children

Journal of Pediatric Endocrinology and Metabolism ◽

10.1515/jpem-2017-0007 ◽

2017 ◽

Vol 30 (8) ◽

Author(s):

Marcella Pedullà ◽

Giuseppina Rosaria Umano ◽

Vincenzo Fierro ◽

Francesco Capuano ◽

Anna Di Sessa ◽

...

Keyword(s):

Risk Factor ◽

Serum Concentration ◽

Subclinical Hypothyroidism ◽

Significant Risk ◽

Thyroid Stimulating Hormone ◽

High Serum ◽

Low Grade ◽

Work Up ◽

Low Grade Inflammation ◽

Serum Tsh

AbstractBackground:Increased thyroid stimulating hormone (TSH) serum concentration can be a marker of subclinical hypothyroidism (SCH) or transient hyperthyrotropinemia. The aim of our study was to evaluate whether high serum TSH concentrations in allergic children could represent true SCH or isolated and transient hyperthyrotropinemia.Methods:We enrolled 620 allergic children (1.11–12.8 years) consecutively attending to our department. They were classified as atopics and non-atopics on the basis of the atopy work-up and, at baseline, they were investigated for thyroid function and low-grade inflammation state. Further, TSH was evaluated after 6 (T1) and 12 (T2) months.Results:Both atopics and non-atopics showed higher SCH prevalence compared to controls (p=0.0055 and p=0.02, respectively), and a significant association between atopy and SCH (OR 10.11, 95% CI 1.36–75.12) was found. Both at T1 and T2, atopics had a significant risk of developing severe SCH compared to non-atopics (RR 1.8, 95% CI 1.39–2.34 and 1.61, 95% CI 1.21–2.14; respectively).Conclusions:Our data may suggest that hyperthyrotropinemia in atopic children could be used as a marker of true SCH.

Download Full-text