Role of corticotropin-releasing factor on bladder function in rats with psychological stress

We previously reported the elevation of plasma interleukin (IL)-6 activity in response to immobilization stress in rats. To investigate the role of peripheral corticotropin-releasing factor (CRF) in this response, we examined the effects of CRF antagonists on immobilization-induced IL-6 response. Intravenous pretreatment with either [d-Phe12,Nle21,38,CαMeLeu37]-anti-human rat (h/r) CRF12—41(1.5 mg/kg) or cyclo(30—33)[d-Phe12, Nle21,38,Glu30,Lys33]-h/rCRF12—41(Astressin, 0.5 mg/kg) attenuated the IL-6 response to immobilization, which confirmed our previous finding that systemic administration of an antiserum against CRF blocked this response. In addition, an intraperitoneal injection of h/rCRF (100 μg/kg) or rat urocortin (10 and 100 μg/kg) increased the plasma IL-6 activity, mimicking the response to immobilization. An intravenous injection of h/rCRF (100 μg/kg) also elevated plasma IL-6 in adrenalectomized rats. These findings suggest that peripheral CRF mediates the plasma IL-6 elevation in response to immobilization.

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Role of the corticotropin-releasing factor receptor-1 in the conditioned preference place induced by morphine in mice

Pharmacological Reports ◽

10.1016/s1734-1140(11)70485-6 ◽

2011 ◽

Vol 63 (1) ◽

pp. 257-258

Author(s):

Carmen Lasheras ◽

Ana González-Cuello ◽

Maria Victoria Milanés ◽

Maria Luisa Laorden

Keyword(s):

Corticotropin Releasing Factor ◽

Conditioned Preference

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Effect of a central CRF antagonist on cardiovascular and thermoregulatory responses induced by stress or IL-1 beta

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1993.265.4.r834 ◽

1993 ◽

Vol 265 (4) ◽

pp. R834-R839 ◽

Cited By ~ 6

Author(s):

T. Nakamori ◽

A. Morimoto ◽

N. Murakami

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Interleukin 1 ◽

Corticotropin Releasing Factor ◽

Mild Stress ◽

Induced Responses ◽

Interleukin 1 Beta ◽

The Central Nervous System ◽

Beta 2

We investigated the role of central corticotropin-releasing factor (CRF) in the development of cardiovascular and thermal responses induced by stress or by interleukin-1 beta (IL-1 beta) in free-moving rats. Intracerebroventricular (icv) injection of alpha-helical CRF9-41 (10 micrograms), a CRF receptor antagonist, significantly attenuated hypertension, tachycardia, and a rise in body temperature induced by cage-switch stress, a mild stress. However, icv injection of alpha-helical CRF9-41 (10 micrograms) had no effect on hypertension, tachycardia, or fever induced by intraperitoneal (ip) injection of IL-1 beta (2 micrograms/kg) or icv prostaglandin E2 (PGE2, 100 ng). In contrast, icv injection of alpha-helical CRF9-41 (10 micrograms) significantly attenuated hypertension, tachycardia, or fever induced by icv injection of IL-1 beta (20 ng). The present results suggest that central CRF has an important role in the development of the cage-switch stress-induced responses, but it does not seem to contribute to the hypertension, tachycardia, and fever induced by ip IL-1 beta or by central PGE2. However, it is possible that when IL-1 beta directly acts on the central nervous system, some of its actions are mediated by central CRF.

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