Effects of Dietary Lysine on Growth Performance, Serum Concentrations of Insulin-Like Growth Factor-I (IGF-I) and IGF-I mRNA Expression in Growing Rabbits

2010 ◽  
Vol 9 (6) ◽  
pp. 887-895 ◽  
Author(s):  
Wen-qian JING ◽  
Fu-chang LI
Keyword(s):  
Growth Factor ◽  
Growth Performance ◽  
Mrna Expression ◽  
Insulin Like Growth Factor ◽  
Serum Concentrations ◽  
Factor I ◽  
Igf I ◽  
Growth Factor I

Hormonal control of insulin-like growth factor-I and growth hormone receptor mRNA expression by porcine hepatocytes in culture

1995 ◽  
Vol 146 (2) ◽  
pp. 239-245 ◽  
Author(s):  
J M Brameld ◽  
P A Weller ◽  
J C Saunders ◽  
P J Buttery ◽  
R S Gilmour
Keyword(s):  
Growth Factor ◽  
Thyroid Hormones ◽  
Mrna Expression ◽  
Culture Medium ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Igf I ◽  
Class 1 ◽  
Growth Factor I ◽  
Porcine Hepatocytes

Abstract The effects of various hormones commonly added to hepatocyte culture media upon the expression of the GH receptor (GHR) and insulin-like growth factor-I (IGF-I) genes in cultured porcine hepatocytes were investigated. Preliminary investigations indicated that there was an absolute requirement only for insulin, with high losses of cell viability upon long term exclusion of insulin from the culture medium. The decline in GHR expression with time in culture was found to be less when high levels of glucose were included in the medium. Therefore the basal culture medium used in these studies was Williams' medium E supplemented with 0·2% (w/v) BSA, 5000 mg glucose/l and 100 nmol porcine insulin/l. The addition of dexamethasone (100 nmol/l) increased the expression of both GHR and IGF-I (class 1 transcripts only) mRNA (P<0·001 and P<0·05 respectively), and resulted in an increased responsiveness of IGF-I mRNA expression to GH (1 μg/ml), when the two were added in combination (although only class 1 transcripts were shown to be statistically significant, P<0·01). The addition of either thyroid hormone (1 nmol/l T3 or T4) alone also increased the expression of GHR mRNA (P<0·01) in addition to the dexamethasone stimulated expression, with T4 appearing to decrease IGF-I expression slightly (P<0·05) (either on its own or with T3). As with dexamethasone, the thyroid hormones increased the response of IGF-I mRNA expression to GH (1 μg/ml) when added in combination with GH (P<0·001). These observations demonstrate one possible mechanism for the interactions of glucocorticoids and thyroid hormones with the GH–IGF axis. Journal of Endocrinology (1995) 146, 239–245

Serum Concentrations of Insulin-Like Growth Factor-I (IGF-I) as a Marker of Liver Fibrosis in Patients With Chronic Hepatitis C

2007 ◽  
Vol 52 (11) ◽  
pp. 3245-3250 ◽  
Author(s):  
Vicente Lorenzo-Zúñiga ◽  
Ramon Bartolí ◽  
Helena Masnou ◽  
Silvia Montoliu ◽  
Rosa M a Morillas ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Growth Factor ◽  
Liver Fibrosis ◽  
Chronic Hepatitis C ◽  
Insulin Like Growth Factor ◽  
Serum Concentrations ◽  
Factor I ◽  
Igf I ◽  
Growth Factor I

Insulin-like growth factor I messenger ribonucleic acid expression in porcine thyroid follicles is regulated by thyrotropin and iodine

1995 ◽  
Vol 132 (5) ◽  
pp. 605-610 ◽  
Author(s):  
Lorenz C Hofbauer ◽  
Michael Rafferzeder ◽  
Onno E Janssen ◽  
Roland Gärtner
Keyword(s):  
Growth Factor ◽  
Mrna Expression ◽  
Ribonucleic Acid ◽  
Cdna Probe ◽  
Northern Hybridization ◽  
Insulin Like Growth Factor ◽  
Messenger Ribonucleic Acid ◽  
Factor I ◽  
Igf I ◽  
Growth Factor I

Hofbauer LC, Rafferzeder M, Janssen OE, Gartner R. Insulin-like growth factor I messenger ribonucleic acid expression in porcine thyroid follicles is regulated by thyrotropin and iodine. Eur J Endocrinol 1995;132:605–10. ISSN 0809–4643 Insulin-like growth factor I (IGF-I) has been shown to be released from thyrocytes in vitro. We investigated IGF-I mRNA expression during treatment with thyrotropin (TSH), forskolin and potassium iodide (KI) in intact porcine thyroid follicles ex vivo. Porcine thyroid follicles were prepared by collagenase digestion and cultured in the presence of TSH, forskolin or KI. After different incubation times, mRNA was isolated and examined by Northern hybridization with a porcine IGF-I cDNA probe of 405 bp in length. In untreated follicles no IGF-I mRNA was found, whereas in follicles stimulated with TSH an IGF-I mRNA of 7.0 kb was detected after 24 h, which persisted for another 24 h. Forskolin treatment mimicked the TSH effect, indicating that IGF-I mRNA expression may be stimulated by the adenylate cyclase pathway. Preincubation of the porcine follicles with KI decreased dose dependently the TSH-induced IGF-I mRNA expression, with complete inhibition at 10 μmol/l KI. These results suggest that TSH acts via the cAMP pathway to enhance IGF-I mRNA expression, which then may lead to an autocrine IGF-I stimulation. The IGF-I mRNA expression is under negative control of iodide. Roland Gärtner, Medizinische Klinik, Ludwig Maximilians University, Ziemssenstrasse 1, 80336 München, Germany

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