scholarly journals Comparison of direct fecal smear microscopy, culture, and polymerase chain reaction for the detection of Blastocystis sp. in human stool samples

2013 ◽  
Vol 6 (10) ◽  
pp. 780-784 ◽  
Author(s):  
Herbert J Santos ◽  
Windell L Rivera
Keyword(s):  
Polymerase Chain Reaction ◽  
Smear Microscopy ◽  
Chain Reaction ◽  
Blastocystis Sp ◽  
Stool Samples ◽  
Polymerase Chain ◽  
Fecal Smear ◽  
Human Stool

scholarly journals First report of Entamoeba moshkovskii in human stool samples from symptomatic and asymptomatic participants in Kenya

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Cecilia Kyany’a ◽  
Fredrick Eyase ◽  
Elizabeth Odundo ◽  
Erick Kipkirui ◽  
Nancy Kipkemoi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Human Gut ◽  
Nested Polymerase Chain Reaction ◽  
First Report ◽  
Stool Samples ◽  
Polymerase Chain ◽  
Entamoeba Moshkovskii ◽  
Human Stool

AbstractEntamoeba moshkovskii is a member of the Entamoeba complex and a colonizer of the human gut. We used nested polymerase chain reaction (PCR) to differentiate Entamoeba species in stool samples that had previously been screened by microscopy. Forty-six samples were tested, 23 of which had previously been identified as Entamoeba complex positive by microscopy. Of the 46 specimens tested, we identified nine (19.5%) as E. moshkovskii-positive. In seven of these nine E. moshkovskii-positive samples, either E. dispar or E. histolytica (or both) were also identified, suggesting that co-infections may be common. E. moshkovskii was also detected in both symptomatic and asymptomatic participants. To the best of our knowledge, this is the first report of E. moshkovskii in Kenya.

Opisthorchis viverrini: Detection by polymerase chain reaction (PCR) in human stool samples

2008 ◽  
Vol 120 (4) ◽  
pp. 353-356 ◽  
Author(s):  
Kanasinakatte R. Umesha ◽  
Sanath Kumar ◽  
Ammini Parvathi ◽  
Kunyarat Duenngai ◽  
Paiboon Sithithaworn ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Opisthorchis Viverrini ◽  
Chain Reaction ◽  
Stool Samples ◽  
Polymerase Chain ◽  
Human Stool

scholarly journals Simultaneous Diagnosis and Species Identification of Microsporidial Infection in Human Stool Samples using Real-Time Polymerase Chain Reaction

2016 ◽  
Vol 3 (4) ◽  
pp. 104-116 ◽  
Author(s):  
Eman Dorry El-Kerdany ◽  
Shwikar Mohamed Ahmed ◽  
Maha Reda Gaafar ◽  
Radwa Galal Diab ◽  
Eman Attia El-Morsy
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Species Identification ◽  
Chain Reaction ◽  
Stool Samples ◽  
Polymerase Chain ◽  
Human Stool

scholarly journals Development of a polymerase chain reaction applicable to rapid and sensitive detection ofClonorchis sinensiseggs in human stool samples

2013 ◽  
Vol 107 (5) ◽  
pp. 253-259 ◽  
Author(s):  
Pyo Yun Cho ◽  
Byoung-Kuk Na ◽  
Kyung Mi Choi ◽  
Jin Su Kim ◽  
Shin-Hyeong Cho ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sensitive Detection ◽  
Chain Reaction ◽  
Stool Samples ◽  
Polymerase Chain ◽  
Human Stool

scholarly journals Diagnosis of Blastocystis sp., Cryptosporidium sp., and Giardia intestinalis by Multiplex PCR: An Optimization Study

2021 ◽  
Author(s):  
Ali Ahmet Kilimcioğlu ◽  
Nogay Girginkardeşler ◽  
Tuba Oyur ◽  
Selin Bölük Sabuncu ◽  
Didem Düzyol Azak ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Dna Isolation ◽  
Giardia Intestinalis ◽  
Molecular Method ◽  
Chain Reaction ◽  
Optimization Study ◽  
Optimized Protocol ◽  
Blastocystis Sp ◽  
Stool Samples ◽  
Polymerase Chain

Objective: It was aimed to develop a new Multiplex Polymerase Chain Reaction (PCR) protocol with isolates obtained from local patients for the diagnosis of Blastocystis sp., Cryptosporidium sp. and Giardia intestinalis, which can cause severe gastrointestinal system complaints especially in immunocompromised patients and children. Method: DNA isolation was performed with a commercial kit from three stool samples of different patients whose microscopic examination showed dense amounts of Blastocystis sp., Cryptosporidium sp. and Giardia intestinalis. First, a special PCR protocol has been developed for each protozoon. Then, the multiplex PCR protocol, in which these three protozoa can be diagnosed together, was optimized. Results: In the multiplex PCR protocol performed after DNA isolation, bands of 95 bp., 227 bp. and 258 bp. were obtained for Cryptosporidium sp., Blastocystis sp. and G. intestinalis, respectively. Conclusion: Blastocystis sp., Cryptosporidium sp. and Giardia intestinalis were diagnosed by multiplex PCR with the original protocol developed. Due to the difficulties in using different methods in parasitological examination, by adding other protozoa important for public health to this optimized protocol, it will be possible to detect a large number of parasites with a single molecular method.

Real-time polymerase chain reaction for detection of Isospora belli in stool samples

2008 ◽  
Vol 61 (3) ◽  
pp. 280-283 ◽  
Author(s):  
Robert-Jan ten Hove ◽  
Lisette van Lieshout ◽  
Eric A.T. Brienen ◽  
M. Arantza Perez ◽  
Jaco J. Verweij
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Chain Reaction ◽  
Stool Samples ◽  
Polymerase Chain
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