scholarly journals Bioassay of Eucalyptus extracts for anticancer activity against Ehrlich ascites carcinoma (eac) cells in Swiss albino mice

2012 ◽  
Vol 2 (5) ◽  
pp. 394-398 ◽  
Author(s):  
Farhadul Islam ◽  
Hasina Khatun ◽  
Soby Ghosh ◽  
MM Ali ◽  
JA Khanam
Keyword(s):  
Anticancer Activity ◽  
Ehrlich Ascites Carcinoma ◽  
Swiss Albino Mice ◽  
Ehrlich Ascites ◽  
Albino Mice ◽  
Eac Cells

Trema orientalis (Linn.) leaves promotes anticancer activity in Ehrlich ascites carcinoma (EAC) in Swiss albino mice

2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Masnoon Kabir ◽  
Abdullah AL-Noman ◽  
Biplab Kumar Dash ◽  
Mahmudul Hasan ◽  
Shahina Akhter ◽  
...  
Keyword(s):  
Body Weight ◽  
Tumor Cell ◽  
Chromatin Condensation ◽  
Hematological Parameters ◽  
Ehrlich Ascites Carcinoma ◽  
Swiss Albino Mice ◽  
Ehrlich Ascites ◽  
Trema Orientalis ◽  
Albino Mice ◽  
Eac Cells

AbstractBackgroundThe in vivo anticancer effect of the Trema orientalis leaves crude methanol extract (TLME) was screened against Ehrlich ascites carcinoma (EAC) in Swiss albino mice.Materials and methodsThe cytotoxic activity of TLME was determined in vitro by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The growth inhibitory activity and morphological alterations were determined by the hemocytometer counting of the EAC cells using trypan blue dye. The apoptotic cells were assessed by DAPI (4′,6-diamidino-2-phenylindole) staining. The hematological and biochemical parameters of experimental mice were also estimated.ResultsAfter treatment with the TLME, the viable tumor cell count, morphological changes and nuclear damages of the EAC cells were observed along with the hematological parameters of the experimental mice. The LD50 of TLME was 3120.650 mg/kg body weight, and this extract was proven to be safe at a dose of as high as 800 mg/kg body weight. The oral administration of the TLME at 400 mg/kg body weight resulted in approximately 59% tumor cell growth inhibition compared with the control mice, with considerable apoptotic features, including membrane blebbing, chromatin condensation, nuclear fragmentation and aggregation of the apoptotic bodies in DAPI staining under a fluorescence microscope. The TLME also dose-dependently restored the altered hematological parameters to approximately normal levels. The TLME exhibited bolstering cytotoxic effect against the EAC cell with the IC50 value of 29.952 ± 1.816 μg/mL.ConclusionThe TLME has potential as a natural anti-cancer product with apoptosis induction property and cytotoxicity against carcinoma cells.

scholarly journals Anticancer potential of Chenopodium album leaf extract against Ehrlich ascites carcinoma cells in Swiss albino mice

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Sohel Rana ◽  
Shakila Rahman ◽  
Samiron Sana ◽  
Tonmoy Kumar Biswas ◽  
Abu Kayes Md. Hashem ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Leaf Extract ◽  
Chenopodium Album ◽  
Ehrlich Ascites Carcinoma ◽  
Cell Growth Inhibition ◽  
Swiss Albino Mice ◽  
Tumor Weight ◽  
Ehrlich Ascites ◽  
Albino Mice ◽  
Eac Cells

Abstract Background Chenopodium album (Family: Amaranthaceae) is a nutritive herb mainly found in Bangladesh and had reported that it is having different bioactive phytochemicals including polyphenols. It has a great popularity for it is traditionally used as a medicine against microorganisms, inflammation, and cancer. We designed this experiment to explore the in vivo anticancer effects of C. album leaf extract against Ehrlich ascites carcinoma (EAC) cells in Swiss albino mice. Results Administration of C. album leaf extract at concentration 200 (mg/kg) and 400 (mg/kg) showed 30.60% and 41.80% cell growth inhibition respectively that were statistically significant (***P < 0.001) and may lead to apoptosis, and the number of apoptotic cells were 37.5 ± 6.22, 54.67 ± 4.76 respectively (***P < 0.001). Plant extract at concentration 200 (mg/kg) and 400 (mg/kg) increased life span 22.27% and 51.07% respectively. Tumor weight decreased 18.50 ± 2.59 g, 13.67 ± 2.25 g respectively compared to the control group, and results were statistically significant (***P < 0.001). Treatment with plant extract 200 (mg/kg) and 400 (mg/kg) restored all biochemical parameters including hemoglobin content, white blood cell (WBC) count, and red blood cell (RBC) count of EAC cells bearing Swiss albino mice. Conclusion Our current findings may suggest that C. album leaf extract showed potent anticancer activity through cell growth inhibition and apoptosis, increased mean survival time, decreased tumor weight, and restored all the biochemical parameters and stimulation of host immunity. As a result, the C. album leaf has taken into consideration as a potent source of bioactive compounds in cancer chemotherapy.

Anticancer activity of pomegranate extract: effect on hematological and antioxidant profile against ehrlich-ascites-carcinoma in Swiss albino mice

2018 ◽  
Vol 19 (3) ◽  
pp. 243-250 ◽  
Author(s):  
V. V. Sathibabu Uddandrao ◽  
Brahmanaidu Parim ◽  
P. R. Nivedha ◽  
K. Swapna ◽  
P. Rameshreddy ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Ehrlich Ascites Carcinoma ◽  
Swiss Albino Mice ◽  
Antioxidant Profile ◽  
Ehrlich Ascites ◽  
Albino Mice
Sign in / Sign up

Export Citation Format

Share Document