Antitumor Efficacy of Silver Nanoparticles Biosynthe-sized from Marine Red Seaweed Halymenia porphyroides Boergesen on Dalton’s Lymphoma Ascites in Mice

The biosynthesized silver nanoparticles from marine red seaweed Halymenia porphyroides against Dalton’s lymphoma ascites (DLA)-induced tumor inoculation was studied for antitumor activity. The biosynthesized silver nanoparticles from marine red seaweed Halymenia porphyroides were given orally to Swiss albino mice (50 mg/kg/day) for 14 days showed a significant reduction in body weight, packed cell volume, andviable tumor cell count when compared to the mice of the DLA control group.The haematological parameters of the treatment group with biosynthesized silver nanoparticles also exhibited increased haemoglobin, RBCs, Platelets, and decreased WBCs compared to the DLA control group of mice. Similarly, the biochemical parameters like total cholesterol, aspartate amino-transferase (AST), alanine aminotransferase (ALT), triglycerides (TL), and alkaline phosphatase (ALP) of the treatment control group with biosynthesized silver nanoparticles reversed the parameters to normal levels compared to the DLA control group of mice. The antitumor efficacy of the biosynthesized silver nanoparticles from Halymenia porphyroides was confirmed based on the haematological, biochemical, life span, packed cell volume, cell count, and histopathological analysis.

Methanolic extract of Moringa oleifera leaves induces Cell cycle arrest and downregulates Mitochondrial membrane potential in Dalton's Lymphoma cells.

Cancer is a group of diseases characterised by abnormal and undifferentiated cell growth that has the potential to spread to other parts of the body. It is the world's second leading cause of death and morbidity. According to the GLOBOCAN 2020 report, out of 19.3 million new cancer cases and 10 million deaths reported, 544352 new cases and 259793 deaths occurred by non-Hodgkin lymphoma (NHLs). Although, numerous therapeutic approaches like, surgery, radiotherapy, chemotherapy and immunotherapy have been developed to treat cancer, limited success has been achieved, possibly due to severe side effects associated with the drugs used during chemotherapy. Therefore, deciphering the novel compound with least side effects and highly potent against cancer is urgently required. In the present study we used leaf extract of M. oleifera, well-known for its anti-cancer efficacy against different cancer cells, however, its effect on Dalton’s lymphoma, a type of spontaneously occurring T cell lymphoma originated in the thymus of DBA mice is seriously lacking. Therefore, present study was aimed to analyze the therapeutic efficacy of M. oleifera against DL cells. Our results show that leaf extract of M. oleifera (MOML) significantly induces morphological changes in DL cells followed by chromatin condensation, nuclear fragmentation, and ROS generation. We also found significant changes in mitochondrial membrane potential (ΔΨm) in a dose dependent manner. Furthermore, apoptosis of DL cells induced by cell cycle arrest at G2/M and S phase suggested that MOML could be used to treat NHL effectively

Cordycepin, an adenosine analog Induces Apoptosis, ROS Generation, and Suppresses Mitochondrial potentials. Exhibits activities against Dalton’s Lymphoma.

Cordycepin exhibits anticancer potentials against various types of cancer cells. However, the multitudes of associated aspects of its anticancer properties against T- cell Lymphoma remains obscure. Based on in vitro studies, we reported that cordycepin exhibits an antineoplastic effect, against Dalton’s Lymphoma (DL) cells. This study illustrates antineoplastic effects and associated mechanisms of cordycepin against tumor cells withdrawn from murine T-cells Lymphoma, designated as Dalton’s Lymphoma. Our study reveals that cordycepin demonstrates tumoricidal activities against tumor cells via activating apoptosis and necrosis, including the surplus level of Reactive oxygen species production, declined mitochondrial membrane potentials, altered cell survival, and chromatin fragmentation. Moreover, this study also reported cordycepin-induced cell cycle arrest in tumor cells which further leads to apoptosis of tumor cells. This investigation elucidates the implication of antitumor actions of cordycepin against T- Cell Lymphoma accompanied by mitochondrial-dependent apoptotic pathway and therefore, have great medicinal significance.