Cryopreservation of human oocytes and the evolution of vitrification technology for this purpose

2011 ◽  
pp. 293-304
Author(s):  
Michael J. Tucker ◽  
Juergen Liebermann
Keyword(s):  
Human Oocytes

Direct Visualization of Metaphase-II Chromosomes in Human Oocytes Under an Inverted Microscope

2011 ◽  
Vol 1 (2) ◽  
pp. 84-88 ◽  
Author(s):  
Atsushi Tanaka ◽  
Motoi Nagayoshi ◽  
Izumi Tanaka ◽  
Hiroshi Kusunoki
Keyword(s):  
Direct Visualization ◽  
Human Oocytes ◽  
Inverted Microscope

scholarly journals High-security closed devices are efficient and safe to protect human oocytes from potential risk of viral contamination during vitrification and storage especially in the COVID-19 pandemic

2021 ◽  
Author(s):  
Eleonora Porcu ◽  
Maria Lucrezia Tranquillo ◽  
Leonardo Notarangelo ◽  
Patrizia Maria Ciotti ◽  
Nilla Calza ◽  
...  
Keyword(s):  
Research Question ◽  
Randomized Study ◽  
Implantation Rate ◽  
University Hospital ◽  
Miscarriage Rate ◽  
Human Oocytes ◽  
High Security ◽  
Group 2 ◽  
And Storage ◽  
Group 1

Abstract Purpose The main purpose and research question of the study are to compare the efficacy of high-security closed versus open devices for human oocytes’ vitrification. Methods A prospective randomized study was conducted. A total of 737 patients attending the Infertility and IVF Unit at S.Orsola University Hospital (Italy) between October 2015 and April 2020 were randomly assigned to two groups. A total of 368 patients were assigned to group 1 (High-Security Vitrification™ - HSV) and 369 to group 2 (Cryotop® open system). Oocyte survival, fertilization, cleavage, pregnancy, implantation, and miscarriage rate were compared between the two groups. Results No statistically significant differences were observed on survival rate (70.3% vs. 73.3%), fertilization rate (70.8% vs. 74.9%), cleavage rate (90.6% vs. 90.3%), pregnancy/transfer ratio (32.0% vs. 31.8%), implantation rate (19.7% vs. 19.9%), nor miscarriage rates (22.1% vs. 21.5%) between the two groups. Women’s mean age in group 1 (36.18 ± 3.92) and group 2 (35.88 ± 3.88) was not significantly different (P = .297). A total of 4029 oocytes were vitrified (1980 and 2049 in groups 1 and 2 respectively). A total of 2564 were warmed (1469 and 1095 in groups 1 and 2 respectively). A total of 1386 morphologically eligible oocytes were inseminated by intracytoplasmic sperm injection (792 and 594 respectively, P = .304). Conclusions The present study shows that the replacement of the open vitrification system by a closed one has no impact on in vitro and in vivo survival, development, pregnancy and implantation rate. Furthermore, to ensure safety, especially during the current COVID-19 pandemic, the use of the closed device eliminates the potential samples’ contamination during vitrification and storage.

Vitrification of human oocytes: The correlation of pre-freezing incubation time with microscopic parameters and final utilization

Cryobiology ◽  
2020 ◽  
Vol 97 ◽  
pp. 295-296
Author(s):  
Irena Kratochvilova ◽  
Olga Teplá ◽  
Jaromír Mašata ◽  
Simona Jirsová ◽  
Michaela Frolíková ◽  
...  
Keyword(s):  
Incubation Time ◽  
Human Oocytes
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