scholarly journals 2279

2017 ◽  
Vol 1 (S1) ◽  
pp. 60-60
Author(s):  
Paula Cooper ◽  
Hsing-Hui Wang ◽  
Meaghan Broman ◽  
Hristos Kaimakliotis ◽  
Bennett Elzey ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Stem Cells ◽  
Cell Culture ◽  
3D Cell Culture ◽  
Autoimmune Inflammation ◽  
Cancer Initiation ◽  
Study Population ◽  
Tissue Recombination ◽  
Survival Mechanisms ◽  
Androgen Independent

OBJECTIVES/SPECIFIC AIMS: The primary goal of this project is to verify murine findings in the human setting. METHODS/STUDY POPULATION: The methods include primary cell isolation and culture, FACS, adoptive transfer, 3D-cell culture, histology, immunofluorescence, xenograft, and tissue recombination. The study population includes patients undergoing radical prostatectomy due to hyperplasia or adjacent bladder or prostate cancer. RESULTS/ANTICIPATED RESULTS: Having verified similar sensitivities to androgen receptor (AR) inhibitors between naive murine and human basal prostate stem cells, we anticipate that autoimmune inflammation in humans affects the response of basal prostate stem cells in a manner similar to the murine setting as well. This includes increased proliferation, differentiation, and response to AR inhibitors. DISCUSSION/SIGNIFICANCE OF IMPACT: The identification of survival mechanisms used by basal prostate stem cells in an androgen deprived environment may give insight to the process by which prostate cancer becomes androgen independent. The effect of inflammation on proliferation, survival, and AR signaling in these cells may also provide information relevant to cancer initiation and progression.

scholarly journals 2185 The effects of autoimmune inflammation on proliferation, differentiation, and androgen receptor signaling in adult prostate stem cells

2018 ◽  
Vol 2 (S1) ◽  
pp. 31-31
Author(s):  
Paula Cooper ◽  
Hsing-Hui Wang ◽  
Meaghan Broman ◽  
Emery Goossens ◽  
Hristos Kaimakliotis ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Stem Cells ◽  
Androgen Receptor ◽  
3D Cell Culture ◽  
Androgen Receptor Signaling ◽  
Autoimmune Inflammation ◽  
Cancer Initiation ◽  
Study Population ◽  
Tissue Recombination ◽  
Survival Mechanisms

OBJECTIVES/SPECIFIC AIMS: The primary goal of this project is to verify findings from a murine prostatitis model in the human setting. METHODS/STUDY POPULATION: Methods include primary cell isolation and culture, FACS, adoptive transfer, 3D cell culture, histology, immunofluorescence, xenograft, and tissue recombination. The study population includes patients undergoing HoLEP or radical prostatectomy due to hyperplasia or adjacent bladder or prostate cancer. RESULTS/ANTICIPATED RESULTS: Having verified similar sensitivities to androgen receptor (AR) inhibitors between naive murine and human basal prostate stem cells, we anticipate that autoimmune inflammation in humans affects the response of basal prostate stem cells in a manner similar to the murine setting as well. This includes increased proliferation, increased differentiation, and decreased response to AR inhibitors. DISCUSSION/SIGNIFICANCE OF IMPACT: The identification of survival mechanisms used by basal prostate stem cells in an androgen deprived environment may give insight to the process by which prostate cancer becomes androgen independent. The effect of inflammation on proliferation, survival, and AR signaling in these cells may also provide information relevant to cancer initiation and progression.

scholarly journals Three-Dimensional Cell Cultures as an In Vitro Tool for Prostate Cancer Modeling and Drug Discovery

2020 ◽  
Vol 21 (18) ◽  
pp. 6806 ◽  
Author(s):  
Fabrizio Fontana ◽  
Michela Raimondi ◽  
Monica Marzagalli ◽  
Michele Sommariva ◽  
Nicoletta Gagliano ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Culture ◽  
Drug Discovery ◽  
Cancer Cells ◽  
Cell Cultures ◽  
Antitumor Agents ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Cancer Modeling

In the last decade, three-dimensional (3D) cell culture technology has gained a lot of interest due to its ability to better recapitulate the in vivo organization and microenvironment of in vitro cultured cancer cells. In particular, 3D tumor models have demonstrated several different characteristics compared with traditional two-dimensional (2D) cultures and have provided an interesting link between the latter and animal experiments. Indeed, 3D cell cultures represent a useful platform for the identification of the biological features of cancer cells as well as for the screening of novel antitumor agents. The present review is aimed at summarizing the most common 3D cell culture methods and applications, with a focus on prostate cancer modeling and drug discovery.

245 MicroRNA-145 mediates the inhibitory effect of adipose-derived stem cells on androgen-independent prostate cancer

2016 ◽  
Vol 15 (3) ◽  
pp. e245
Author(s):  
K. Takahara ◽  
T. Inamoto ◽  
N. Ibuki ◽  
T. Uchimoto ◽  
K. Saito ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Stem Cells ◽  
Inhibitory Effect ◽  
Adipose Derived Stem Cells ◽  
Androgen Independent

scholarly journals 3D Spheroid Cell Cultures and Their Role in Bone Regeneration: a Systematic Review

2021 ◽  
pp. 178-191
Author(s):  
María Verónica Cuevas-González ◽  
Fernando Suaste-Olmos ◽  
Juan Carlos Cuevas-González ◽  
Marco Antonio Álvarez-Pérez
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Cell Culture ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Clinical Application ◽  
3D Cell Culture ◽  
The United States ◽  
Spheroid Cell Culture

Recently, the 3D spheroid cell culture application has been extensively used in the treatment of bone defects. A wide variety of methodologies have been used, which has made the comparison of results complex. Therefore, this systematic review has two aims: (i) to perform an analysis focused on the role of 3D spheroid cell culture in bone regeneration strategies; and (ii) address the main challenges in clinical application. A search of the following keywords "3D cell culture", "spheroid", and "bone regeneration" was carried out in the PubMed, Scopus, and ScienceDirect databases and limited to the years 2010-2020. Studies were included if their primary objective was the behavior of cell aggregates to formed spheroids structures by different 3D cell culture techniques focused on the regeneration of bone tissue. To address the risk of bias for in vitro studies, the United States national toxicology program tool was applied, and descriptive statistics of the data were performed, with the SPSS V.22 program. A total of 16 studies were included, which met the established criteria corresponding to in vitro and in vitro/in vivo studies; most of these studies used stem cells for the 3D cell spheroids. The most often methods used for the 3D formation were low adherence surface and rotational methods, moreover, mesenchymal stem cells were the cell line most frequently used because of their regenerative potential in the field of bone tissue engineering. Although the advances in research on the potential use of 3D spheroids in bone regeneration have made great strides, the constant innovation in cell spheroid formation methodologies means that clinical application remains in the future as strategy for 3D tissue bioprinting.

scholarly journals Basal epithelial stem cells are efficient targets for prostate cancer initiation

2010 ◽  
Vol 107 (6) ◽  
pp. 2610-2615 ◽  
Author(s):  
D. A. Lawson ◽  
Y. Zong ◽  
S. Memarzadeh ◽  
L. Xin ◽  
J. Huang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Stem Cells ◽  
Epithelial Stem Cells ◽  
Cancer Initiation

scholarly journals 3D Cell Culture Model for Prostate Cancer Cells to Mimic Inflammatory Microenvironment

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1555 ◽  
Author(s):  
Gizem Gulevin Takir ◽  
Bilge Debelec-Butuner ◽  
Kemal Sami Korkmaz
Keyword(s):  
Prostate Cancer ◽  
Cell Culture ◽  
Cancer Cells ◽  
Cancer Progression ◽  
3D Cell Culture ◽  
Prostate Cancer Cells ◽  
Cell Culture Model ◽  
Normal Prostate ◽  
Inflammatory Microenvironment ◽  
Culture Model

The studies on the relationship between inflammation and cancer progression have been mostly carried out with monolayer cell cultures in vitro, which can be insufficient to mimic tumor tissue. Here, we established a three-dimensional (3D) cell culture model of inflammatory microenvironment for prostate cancer cells to better evaluate the role of inflammation in prostate carcinogenesis. Formation of the cell spheroids has been achieved for LNCaP, Du145, LNCaP-104r2 prostate cancer cell lines but not for RWPE1 normal prostate epithelial cell and PC3 by using 3D Petri Dish®. We also showed that cells in inflammatory conditioned media might have a different response based on the culturing method. Overall, we are suggesting that 3D cell culture model can be a useful tool to study molecular alterations on proliferation and migration/invasion of tumor cells related to inflammation.

Non-traditional 3D cell culture scaffold in canine mesenchymal stem cells

Cytotherapy ◽  
2015 ◽  
Vol 17 (6) ◽  
pp. S71
Author(s):  
Shelly Zacharias ◽  
Diana Thomas ◽  
Jennifer Rodenberg ◽  
Steven Charlebois
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
3D Cell Culture
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