scholarly journals Evaluation of role of concentration and molecular weight of oat β-glucan in determining effect of viscosity on plasma glucose and insulin following an oral glucose load

2000 ◽  
Vol 84 (1) ◽  
pp. 19-23 ◽  
Author(s):  
P. J. Wood ◽  
M. U. Beer ◽  
G. Butler
Keyword(s):  
Molecular Weight ◽  
Blood Glucose ◽  
High Performance ◽  
Size Exclusion ◽  
Postprandial Blood Glucose ◽  
Oral Glucose ◽  
Oral Glucose Load ◽  
Glucose Load ◽  
Exclusion Chromatography ◽  
Insulin Responses

Data from clinical studies established that there was an inverse linear relationship between measures of postprandial blood glucose and insulin responses to an oral glucose load, consumed in a drink, and the logarithm of viscosity of the drink. These data have been re-analysed using concentration and molecular weight as the dependent variables. Molecular weight (M) of the β-glucans used was determined using high-performance size exclusion chromatography equipped with a triple detector system of right angle light scattering, viscometry and refractive index. A significant relationship between changes in peak blood glucose and a combination of logarithm of the concentration and logarithm of M was found.

A new concept for the evaluation of the blood glucose and insulin responses of healthy subjects following an oral glucose load

1974 ◽  
Vol 11 (4) ◽  
pp. 265-276 ◽  
Author(s):  
Olga Szabo ◽  
Werner Oppermann ◽  
Rafael A. Camerini-Dávalos ◽  
Carl Victor
Keyword(s):  
Blood Glucose ◽  
Healthy Subjects ◽  
Oral Glucose ◽  
Oral Glucose Load ◽  
Glucose Load ◽  
Insulin Responses

Comparison of exopolysaccharides from mucoid and nonmucoid strains of Clavibacter michiganensis subspecies sepedonicus

1993 ◽  
Vol 39 (3) ◽  
pp. 291-296 ◽  
Author(s):  
Paul J. Henningson ◽  
Neil C. Gudmestad
Keyword(s):  
Molecular Weight ◽  
High Performance ◽  
Size Exclusion ◽  
Enzyme Linked Immunosorbent Assay ◽  
Low Molecular Weight ◽  
Neutral Sugar ◽  
Clavibacter Michiganensis ◽  
Serological Reaction ◽  
Molecular Weights ◽  
Exclusion Chromatography

The exopolysaccharides produced by six strains of Clavibacter michiganensis ssp. sepedonicus were isolated and purified by liquid chromatography. Neutral sugar composition and molecular weights were determined for each polysaccharide fraction, using gas chromatography and high-performance size-exclusion chromatography. The serological reaction of each fraction was tested using enzyme-linked immunosorbent assay. Exopolysaccharide from nonmucoid strains contained only low molecular weight polysaccharides (1.5 × 103 to 1.1 × 104). Exopolysaccharide from mucoid and intermediate strains could be separated into low (4.0 × 103 to 1.1 × 104) molecular weight and high (5.0 × 105 to 1.6 × 106) molecular weight fractions. High molecular weight polysaccharides were composed almost exclusively of galactose, glucose, and fucose. The ratios of these sugars were highly variable among strains. Low molecular weight polysaccharides were primarily composed of galactose with significant and varying amounts of glucose, rhamnose, mannose, and ribose. All polysaccharide fractions except one, produced by a nonmucoid strain, reacted in the immunoassay test.Key words: exopolysaccharide, polysaccharide, Clavibacter, michiganensis, sepedonicus.

High Performance Size Exclusion Chromatograph with Computerized Data Reduction for Analysis of Polyethylene and Oligomers

1981 ◽  
Vol 64 (4) ◽  
pp. 999-1007
Author(s):  
Roger C Snyder ◽  
Charles V Breder
Keyword(s):  
Molecular Weight ◽  
High Performance ◽  
Average Molecular Weight ◽  
Porous Silica ◽  
Size Exclusion ◽  
Dead Volume ◽  
Shape Distortion ◽  
Molecular Weight Range ◽  
Liquid Chromatograph ◽  
Exclusion Chromatography

Abstract A liquid chromatograph is described for the size exclusion chromatography (SEC) of polyethylene and oligomers. The rigid column packing material was 10 µm diameter porous silica. An infrared spectrophotometer was used as the detector. The chromatograph was designed and operated to minimize the degree of chromatographic peak shape distortion resulting from factors such as system dead volume, detector cell design, sample injection volume, and sample concentration. To compensate for peak spreading and skewing, the chromatographic data were analyzed with an IBM 370/168 computer using an APL program. The molecular weight range covered by this SEC system was 100-500 000. Precision and accuracy of Jhe calculated weight average molecular weight (Mw) values for polyethylene standards over this range were ±3 and s±4%, respectively.

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