scholarly journals DNA methylation and one-carbon metabolism related nutrients and polymorphisms: analysis after mandatory flour fortification with folic acid

2019 ◽  
Vol 123 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Josiane Steluti ◽  
Cecília Zanin Palchetti ◽  
Andreia Machado Miranda ◽  
Regina Mara Fisberg ◽  
Dirce Maria Marchioni
Keyword(s):  
Dna Methylation ◽  
Folic Acid ◽  
Carbon Metabolism ◽  
The Elderly ◽  
Cross Sectional Study ◽  
Global Dna Methylation ◽  
Cross Sectional ◽  
Absolute Change ◽  
Global Status ◽  
One Carbon Metabolism

AbstractThere is a growing research interest in determining whether changes in the global status of DNA methylation are related to the environment, in particular, to one-carbon metabolism. So, our aim was to investigate the effect of dietary methyl-group donor intake (methionine, folate, choline, betaine, vitamins B2, B6 and B12), biomarkers (total folate, unmetabolised folic acid (FA), 5-methyltetrahydrofolate, homocysteine, vitamins B6 and B12 concentrations) and genetic variants (polymorphisms involved in one-carbon metabolism) on global DNA methylation in a population exposed to mandatory flour fortification with FA. A cross-sectional study of health and living conditions was conducted among a representative sample of residents in São Paulo, Brazil. The mean of global DNA methylation was lower in young people than in adults and the elderly (P = 0·049). No differences between genotypes of polymorphism and global DNA methylation mean were identified. We observed that the increase in betaine intake led to an absolute change in percentage of DNA methylation (β = 0·0005, P = 0·024) using multiple regression. Betaine intake alone was associated with an absolute change in percentage of global DNA methylation. The study did not find an association between global DNA methylation and folate status even in a population exposed to mandatory flour fortification with FA.

Metformin regulates global DNA methylation via mitochondrial one-carbon metabolism

Oncogene ◽  
2017 ◽  
Vol 37 (7) ◽  
pp. 963-970 ◽  
Author(s):  
E Cuyàs ◽  
S Fernández-Arroyo ◽  
S Verdura ◽  
R Á-F García ◽  
J Stursa ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Carbon Metabolism ◽  
Global Dna Methylation ◽  
One Carbon Metabolism

scholarly journals A cross-sectional study of global DNA methylation and risk of colorectal adenoma

BMC Cancer ◽  
2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Will D King ◽  
Janet E Ashbury ◽  
Sherryl A Taylor ◽  
M Yat Tse ◽  
Stephen C Pang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Colorectal Adenoma ◽  
Cross Sectional Study ◽  
Global Dna Methylation ◽  
Sectional Study ◽  
Cross Sectional

scholarly journals Vitamin B6, B12, Asam Folat, Tekanan Darah dan Demensia pada Lanjut Usia

2020 ◽  
Vol 4 (2) ◽  
pp. 123
Author(s):  
Bekti Krisdyana ◽  
Diffah Hanim ◽  
Sugiarto Sugiarto
Keyword(s):  
Blood Pressure ◽  
Folic Acid ◽  
Junior High School ◽  
Random Sampling ◽  
Vitamin B6 ◽  
Sampling Technique ◽  
The Elderly ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Cluster Random Sampling

Background: Dementia is a multifactorial disease due to genetic and environmental influences. Lack of intake of vitamins B6, B12, folic acid and blood pressure are one of the environmental factors that cause dementia.Objective: To analyze the relationship between the adequacy of vitamins B6, B12, folic acid and blood pressure with dementia in the elderlyMethod: This research was a cross sectional study, which was carried out in Tulungagung Regency. The sampling technique was cluster random sampling and a sample size of 100 elderly from 7 elderly Posyandu in Tulungagung Regency, with independent variables such as adequacy of vitamin B6, B12, folic acid and blood pressure, while the dependent variable was dementia. Data were analyzed with chi square testResults: Respondents in this study were generally aged 60-74 years (70.0%), women (48.0%), were educated in elementary / junior high school (60.0%) and did not work (69.0%). Most respondents have insufficient vitamin B6, B12 and folic acid in the less category, with percentages of 57.0%, 54% and 64%, respectively. There was an association between the adequacy of vitamins B6, B12 and folic acid with dementia in the elderly (OR=2.302; 95% CI=1.011-5.241; p=0.045; OR=2.397; 95% CI=1.060-5.422; p=0.034; OR=2.516; 95% CI=1.015-6.238; p=0.043). Blood pressure is associated with dementia in the elderly (OR=3.000; 95% CI=1.288-6.988; p=0.010). The average consumption of vitamin B6, B12 and folic acid in the normal group was higher than the dementia group, with a difference of 0.16 ± 0.04 mg, 0.54 ± 0.05 mcg and 65.89 ± 4.95 respectively mcgConclusionABSTRAKLatar Belakang: Demensia merupakan penyakit multifaktorial karena pengaruh genetik dan lingkungan. Minimnya asupan vitamin B6, B12, asam folat dan tekanan darah menjadi salah satu faktor lingkungan yang menyebabkan demensia.Tujuan:  Tujuan penelitian ini adalah menganalisis hubungan antara kecukupan vitamin B6, B12, asam folat dan tekanan darah dengan demensia pada lanjut usiaMetode: Penelitian ini berupa penelitian cross sectional, yang dilakukan di Kabupaten Tulungagung. Teknik sampling dengan cluster random sampling dan besar sampel 100 lansia dari 7 posyandu lansia di Kabupaten Tulungagung, dengan variabel bebas berupa kecukupan vitamin B6, B12, asam folat dan tekanan darah, sedangkan variabel terikatnya adalah demensia. Data dianalisis dengan uji chi squareHasil: Responden penelitian ini umumnya berusia 60-74 tahun (70,0 %), wanita (48,0 %), berpendidikan terakhir SD/SMP (60,0 %) dan tidak bekerja (69,0 %). Kebanyakan responden memiliki kecukupan vitamin B6, B12 dan asam folat dalam kategori kurang, dengan persentase masing-masing  57,0 %, 54 % dan 64 %. Ditemukan hubungan antara kecukupan vitamin B6, B12 dan asam folat dengan demensia pada lanjut usia (OR=2,302, 95% CI=1,011-5,241, p=0,045; OR=2,397, 95% CI=1,060-5,422, p=0,034; OR=2,516,95% CI=1,015-6,238, p=0,043). Tekanan darah berhubungan dengan demensia pada lanjut usia (OR=3,000,95% CI=1,288-6,988,p=0,010). Rata-rata konsumsi vitamin B6, B12 dan asam folat kelompok normal lebih tinggi dibandingkan kelompok demensia, dengan selisih masing-masing sebesar 0,16±0,04 mg, 0,54±0,05 mcg dan 65,89 ±4,95 mcg.Kesimpulan: Terdapat hubungan antara kecukupan vitamin B6, B12, asam folat dan tekanan darah dengan demensia pada lanjut usia. 

scholarly journals O6D.2 Evidence of dna methylation changes by carbon nanotubes in a translational study design

2019 ◽  
Vol 76 (Suppl 1) ◽  
pp. A57.2-A57
Author(s):  
Manosij Ghosh ◽  
Deniz Öner ◽  
Lode Godderis ◽  
Peter Hoet
Keyword(s):  
Dna Methylation ◽  
Cross Sectional Study ◽  
Methylation Pattern ◽  
Global Dna Methylation ◽  
Limited Information ◽  
Cross Sectional ◽  
Translational Study ◽  
Exposed Workers

IntroductionWhile studies have addressed genotoxic effects of CNT, only limited information are available on epigenetic effects. We designed a study to investigate DNA methylation alterations in vitro, in vivo and in occupationally exposed workers.Material and methodsIn vitro studies were performed in 16-HBE and THP-1 cells. For the in vivo study, BALB/c mice were administered intratracheally with single-wall CNT (SWCNTs) and multi-wall (MWCNTs) at high (2.5 mg/kg) and low (0.25 mg/kg) doses. For the cross sectional study, 24 workers exposed to aggregates of MWCNT of 500 nm–100 µm with concentrations of 4.6–42.6 µg/m3 and 43 unexposed referents were recruited. Global DNA methylation and demethylation patterns were analysed by LC-MS/MS. Methylation of specific genes was measured by Pyromark 24® (Qiagen). Genome-wide assessment of DNA methylation was performed with Infinium HumanMethylation450 BeadChip Array.ResultsIn general, we did not find global DNA methylation alteration for both CNTs. In 16-HBE cells, differentially methylated and expressed genes (MWCNTs>SWCNTs) from p53 signalling, DNA damage repair and cell cycle pathways were observed. In THP-1 cells, CNTs induced promoter-specific methylation of genes involved in several signaling cascade, vascular endothelial growth factor and platelet activation pathways. In lungs of BALB/c mice CNTs affected methylation of ATM gene. Finally, analysis of gene-specific DNA methylation in exposed workers revealed significant changes for DNMT1, ATM, SKI, and HDAC4 promoter CpGs.ConclusionsEpigenetic changes seem to occur at sub cyto-genotoxic concentrations in vitro. Alteration in DNA methylation pattern could be a natural reaction of cells but could also silence critical genes and reprogram cellular functions.

One-carbon metabolism and global DNA methylation in mothers of individuals with Down syndrome

Human Cell ◽  
2021 ◽  
Author(s):  
Cristiani Cortez Mendes ◽  
Bruna Lancia Zampieri ◽  
Lidia Maria Rebolho Batista Arantes ◽  
Matias Eliseo Melendez ◽  
Joice Matos Biselli ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Down Syndrome ◽  
Carbon Metabolism ◽  
Global Dna Methylation ◽  
One Carbon Metabolism
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