Bacillus thuringiensisisolates from the Philippines: habitat distribution, δ-endotoxin diversity, and toxicity to rice stem borers (Lepidoptera: Pyralidae)

1998 ◽  
Vol 88 (3) ◽  
pp. 335-342 ◽  
Author(s):  
W. Theunis ◽  
R.M. Aguda ◽  
W.T. Cruz ◽  
C. Decock ◽  
M. Peferoen ◽  
...  
Keyword(s):  
Rice Field ◽  
Polyclonal Antibodies ◽  
Stem Borer ◽  
The Philippines ◽  
Positive Sample ◽  
Rice Grain ◽  
Scirpophaga Incertulas ◽  
Yellow Stem Borer ◽  
Pcr Products ◽  
Grain Dust

AbstractBacillus thuringiensisBerliner isolates were detected in 57% of 801 samples of rice grain dust, soil, rice field arthropods, and miscellaneous habitats (rice straw compost and mammal faeces) collected at 100 sites in the Philippines. The collection yielded 3950 isolates ofB. thuringiensis(8.7 isolates/positive sample). Grain dust from rice mills was the richest source (63% of the samples were positive, with 10.2 isolates/positive sample), followed by rice field arthropods, soil, and miscellaneous habitats. Polyclonal antibodies to six δ-endotoxin groups (Cry1A, Cry1B, Cry1C, Cry1D, Cry1E, and Cry3A) were used in enzyme-linked immunosorbent assays (ELISA) to characterize the toxins produced by each isolate. Subsamples of isolates representing the diversity of isolate sources and δ-endotoxin profiles were bioassayed against the yellow stem borer,Scirpophaga incertulas(Walker) and striped stem borer,Chilo suppressalis(Walker). Eighteen isolates highly toxic to both species were selected for characterization of δ-endotoxin genes by polymerase chain reaction (PCR) with primers specific to 14 genes or gene subfamilies, and Western blotting with Cry2A antibodies. At least two novel δ-endotoxin genes, related tocry1Bandcry1F, were detected by DNA sequencing of PCR products.

scholarly journals Farmer participatory assessment of pest management modules for yellow stem borer and leaf folder in summer rice

2020 ◽  
Vol 57 (4) ◽  
pp. 315-319
Author(s):  
US Nayak ◽  
A Das
Keyword(s):  
Pest Management ◽  
Rice Field ◽  
Foliar Spray ◽  
Stem Borer ◽  
Mass Trapping ◽  
Infestation Level ◽  
Scirpophaga Incertulas ◽  
Yellow Stem Borer ◽  
Participatory Assessment ◽  
Major Pest

Rice yellow stem borer, Scirpophaga incertulas (Walker) (Pyralidae: Lepidoptera) is the major pest of rice that causes extensive damage to the crop and its infestation is more severe in summer rice. Field experiment for two consecutive seasons (summer 2014 and 2015) at Bhadrak, Odisha (India) was carried out to assess the relative efficacy of some modules pest management against yellow stem borer and leaf folder in rice. The pest management module comprising of nursery treatment with fipronil 03 G @ 100 g/ cent nursery area at 7 days before transplanting, clipping of leaf tips at transplanting, installation of pheromone traps for mass trapping @ 50/ ha, soil application of chlorantraniliprole 0.4 G @ 10 kg/ ha at 30 DAT and foliar spray of indoxacarb 14.5 SC @500 ml/ ha at 60 DAT minimised the stem borer damage substantially (76.40 and 76.15 % reduction of dead heart and white ear head, respectively over control) and contributed to the highest grain yield ( 42.86 % increase over control) with maximum net return (Rs. 19899.05 per hectare) and B :C ratio (1.46). The current pest management module also reduced the infestation level of leaf folder in rice (60.72 % reduction over the untreated control).

Control of yellow stem borer, Scirpophaga incertulas (Lepidoptera: Pyralidae) by mating disruption on rice in India: effect of unnatural pheromone blends and application time on efficacy

1996 ◽  
Vol 86 (5) ◽  
pp. 515-524 ◽  
Author(s):  
A. Cork ◽  
K. De Souza ◽  
K. Krishnaiah ◽  
D.V.S.S.R. Kumar ◽  
A. Ashok Reddy ◽  
...  
Keyword(s):  
Mating Disruption ◽  
Cumulative Effect ◽  
Stem Borer ◽  
Pheromone Trap ◽  
Rice Grain ◽  
Mediated Communication ◽  
Scirpophaga Incertulas ◽  
Yellow Stem Borer ◽  
Significant Difference ◽  
Zero Order Release

AbstractSingle applications of slow-release pheromone formulations were used to control yellow stem borer, Scirpophaga incertulas Walker, by mating disruption in two trials on rice in India. The first trial compared the efficacy of two formulations containing 1:10:1 and 1:10:0 ratios of (Z)-9-hexadecenal, (Z)-ll-hexadecenal and (Z)-9-octadecenal, components of the S. incertulas pheromone, and a commercially-available formulation, Selibate CS, containing the related Chilo suppressalis Walker pheromone, a 1:10:1 blend of (Z)-9-hexadecenal, (Z)-ll-hexadecenal and (Z)-13-octadecenal, with farmers' practice plots treated with insecticides. Pheromone trap catches indicated that in each of the pheromone-treated plots the catches of male moths were reduced by up to 98% compared with catches in the insecticide-treated plots suggesting that pheromone-mediated communication was disrupted. Larval damage ranged from 5.7 to 8.1% white heads (WH) in the insecticide-treated plots compared to a significantly reduced range of 2.1 to 2.4% WH in the pheromone-treated plots. There was no significant difference between the damage estimates recorded for each of the different pheromone treatments. A second trial compared the effect on efficacy of applying the two-component pheromone formulation on different dates, 9–12 and 39–44 days after transplanting. Both pheromone treatments gave significantly lower levels of WH damage compared to equivalent insecticide treated plots; 1.8, 2.0, and 6% respectively. In both trials rice grain yields increased by between 5 and 12% in pheromone-treated plots compared to insecticide treated plots, which was due, at least in part, to the cumulative effect of reduced stem borer damage in both the tillering and reproductive stages of the crop cycle. The formulations exhibited pseudo-zero order release rates with field lives of between 70 and 90 days.

Natural parasitism of eggs of yellow stem borer, Scirpophaga incertulas Walker (Lepidoptera: Crambidae) in rice ecosystem at Tiruchirappalli, Tamil Nadu

ENTOMON ◽  
2020 ◽  
Vol 45 (3) ◽  
pp. 181-188
Author(s):  
T. Sharmitha ◽  
C. Gailce Leo Justin ◽  
S. Sheeba Joyce Roseleen ◽  
P. Yasodha
Keyword(s):  
Field Study ◽  
Tamil Nadu ◽  
Host Density ◽  
Stem Borer ◽  
Scirpophaga Incertulas ◽  
Egg Masses ◽  
Yellow Stem Borer ◽  
Rice Ecosystem ◽  
Natural Parasitism

Three species of parasitoids viz., Telenomus dignus Gahan, Trichogramma japonicum, Ishii and Tetrastichus schoenobii Ferriere were recorded from the egg masses of rice yellow stem borer, Scirpophaga incertulas (Walker) in a field study. The extent of parasitism was high during Rabi (43.33 – 93.33 %) and low during Kharif (0 - 40.00 %). Parasitism by T. dignus was maximum in October (50.00 %), T. japonicum, in November (23.08 %) and T. schoenobii in February (55.55 %). dignus and T. schoenobii in combination parasitized maximum number of egg masses (41.82 %). Multiple parasitism by the three species was high in December (8.33 %) and January (7.14%). Parasitic potential was maximum, when T. schoenobii alone parasitised the egg masses followed by T. dignus and T. schoenobii in combination. Host density in the field influenced the extent of parasitism.

scholarly journals Evaluation of different rice genotype against yellow stem borer (Scirpophaga incertulas Walker)

2020 ◽  
Vol 8 (2) ◽  
pp. 1510-1514
Author(s):  
Munna Yadav ◽  
Rajendra Prasad ◽  
Rabindra Prasad ◽  
Aroondhaty Choudhary ◽  
Dhyanananda Kumari
Keyword(s):  
Stem Borer ◽  
Scirpophaga Incertulas ◽  
Yellow Stem Borer ◽  
Rice Genotype

scholarly journals Effect of elevated carbon dioxide on biology and morphometric parameters of yellow stem borer, Scirpophaga incertulas infesting rice (Oryza sativa)

2022 ◽  
Vol 24 (1) ◽  
Author(s):  
GOURI SHANKAR GIRI ◽  
S. V. S. RAJU ◽  
S. D. MOHAPATRA ◽  
MUNMUN MOHAPATRA
Keyword(s):  
Carbon Dioxide ◽  
Larval Instar ◽  
Co2 Concentration ◽  
Elevated Carbon Dioxide ◽  
Stem Borer ◽  
Developmental Period ◽  
Morphometric Parameters ◽  
Scirpophaga Incertulas ◽  
Yellow Stem Borer ◽  
Co2 Concentrations

An experiment was conducted at Research Farm, National Rice Research Institute, Cuttack, Odisha, India to quantify the effect of elevated carbon dioxide (CO2) concentrations on the biology and morphometric parameters of yellow stem borer (Scirpophaga incertulas, Pyralidae, Lepidoptera). Yellow stem borer is one of the major pest of rice in the whole rice growing regions of South East Asia. The effect of three carbon dioxide concentrations i.e. 410 ppm (ambient), 550 ppm and 700 ppm on the duration of the developmental period as well as morphometric parameters of each stage of the lifecycle of the pest was analysed. It was found that, there was an increase in the duration of the developmental period of each stage of life cycle as the concentration of CO2 increases. However, the life span of the adult moth was significantly lower under the elevated CO2 concentrations when compared with ambient CO2 concentration. Morphometric parameters viz., mean length, width and weight of each larval instar, pupa and adult were found to be significantly higher in elevated concentrations of CO2 as compared to ambient concentration.

scholarly journals Identification of Possible Sex Pheromone of the Yellow Stem Borer Moth, Scirpophaga incertulas (WALKER)(Lepidoptera : Pyralidae)

1985 ◽  
Vol 20 (3) ◽  
pp. 357-359 ◽  
Author(s):  
Sadahiro TATSUKI ◽  
Hajime SUGIE ◽  
Kenji USUI ◽  
Jun-ichi FUKAMI ◽  
Muhamad Hoedaya SUMARTAPUTRA ◽  
...  
Keyword(s):  
Sex Pheromone ◽  
Stem Borer ◽  
Scirpophaga Incertulas ◽  
Yellow Stem Borer ◽  
Lepidoptera Pyralidae

scholarly journals INSIDENSI PENGGEREK BATANG PADI KUNING (Schirpophaga incertulas Walker) PADA TIGA VARIETAS PADI : KASUS DI DESA KEMUMU BENGKULU UTARA

2018 ◽  
Vol 20 (2) ◽  
pp. 40-45
Author(s):  
Irma Cahyoko ◽  
Dwinardi Apriyanto ◽  
Kanang S. Hindarto
Keyword(s):  
Grain Yield ◽  
Rice Field ◽  
Yield Loss ◽  
Stem Borer ◽  
Egg Mass ◽  
Cumulative Number ◽  
Large Area ◽  
Rice Varieties ◽  
Yellow Stem Borer ◽  
Significant Difference

[INCIDENCE OF THE RICE YELLOW STEM BORER (Schirpophaga incertulas Walker) ON THREE RICE VARIETIES IN KEMUMU, NORTH BENGKULU]. Rice yellow stem borer (RYSB), Schirpophaga incertulas Walker, often causes yield loss in irrigated rice field. Dead heard and white ear head are the common symptomps resulted from the larval damage. The use of resistant varieties may reduce the risk of yield loss, but the resistance may lose quickly as the varieties are continually grown in a large area. The assessment of the insect incidence in rice field is necessary in order to determine the status of the pest for certain period of time. The objective of the study was to determine the incidence and damage incurred by RYSB in three rice varieties, Mekongga, Cibogo, and Situ Bagendit at farmer rice fields. A survey was conducted in December 2017-February 2018 in Kemumu, North Bengkulu Regency. Observation of RYSB was made on randomly selected 30 hills from + 1 ha area for each variety that was located adjacent to each other. Grain yield was measured from the sampled hills and from 3 plots of 9 m2 . Weight of 1000 grain was measured from grain of three sampled hills. The results revealed that the incidence of RYSB was low, as indicated by the presence of egg mass and larvae of RYSB, and no significant difference was found among the three varieties. Nevertheless, the average cumulative number and percentage of dead heart and white ear head was significantly lower in Mekongga than those of Cibogo and Situ Bagendit. In term of grain yield, however, Mekongga outperformed the other two varieties. The low incidence of RYSB might suggest that growing different variety practiced by local farmers was able to suppress pest population, and therefore it should be implemented in all main areas of rice production.

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