Identification and analysis of chemosensory genes encoding odorant-binding proteins, chemosensory proteins and sensory neuron membrane proteins in the antennae of Lissorhoptrus oryzophilus

2021 ◽  
pp. 1-11
Author(s):  
Yu Pan ◽  
Xinxin Zhang ◽  
Zhun Wang ◽  
Lizhong Qi ◽  
Xinsheng Zhang ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Sensory Neuron ◽  
Binding Proteins ◽  
Mate Location ◽  
Lissorhoptrus Oryzophilus ◽  
Neuron Membrane ◽  
Odorant Binding Proteins ◽  
Chemosensory Proteins ◽  
Chemosensory Genes ◽  
Odorant Binding

Abstract The rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), is a destructive pest that causes damage to rice crops worldwide. The olfactory system is critical for host or mate location by weevils, but only limited information about the molecular mechanism of olfaction-related behaviour has been reported in this insect. In this study, we conducted SMRT-seq transcriptome analysis and obtained 54,378 transcripts, 38,706 of which were annotated. Based on these annotations, we identified 40 candidate chemosensory genes, including 31 odorant-binding proteins (OBPs), six chemosensory proteins (CSPs) and three sensory neuron membrane proteins (SNMPs). Phylogenetic analysis showed that LoryOBPs, LoryCSPs and LorySNMPs were distributed in various clades. The results of tissue expression patterns indicated that LoryOBPs were highly abundant in the antennae, whereas LoryCSPs were highly abundant not only in the antennae but also in the abdomen, head and wings. Our findings substantially expand the gene database of L. oryzophilus and may serve as a basis for identifying novel targets to disrupt key olfactory genes, potentially providing an eco-friendly strategy to control this pest in the future.

scholarly journals Antennal Transcriptome Analysis and Identification of Candidate Chemosensory Genes of the Harlequin Ladybird Beetle, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae)

Insects ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 209
Author(s):  
Gabriele Rondoni ◽  
Alessandro Roman ◽  
Camille Meslin ◽  
Nicolas Montagné ◽  
Eric Conti ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Harmonia Axyridis ◽  
Differential Expression Analysis ◽  
Mate Location ◽  
Gene Repertoire ◽  
Odorant Binding Proteins ◽  
Chemosensory Genes ◽  
Males And Females ◽  
Odorant Binding ◽  
Harmonia Axyridis Pallas

In predatory ladybirds (Coleoptera: Coccinellidae), antennae are important for chemosensory reception used during food and mate location, and for finding a suitable oviposition habitat. Based on NextSeq 550 Illumina sequencing, we assembled the antennal transcriptome of mated Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) males and females and described the first chemosensory gene repertoire expressed in this species. We annotated candidate chemosensory sequences encoding 26 odorant receptors (including the coreceptor, Orco), 17 gustatory receptors, 27 ionotropic receptors, 31 odorant-binding proteins, 12 chemosensory proteins, and 4 sensory neuron membrane proteins. Maximum-likelihood phylogenetic analyses allowed to assign candidate H. axyridis chemosensory genes to previously described groups in each of these families. Differential expression analysis between males and females revealed low variability between sexes, possibly reflecting the known absence of relevant sexual dimorphism in the structure of the antennae and in the distribution and abundance of the sensilla. However, we revealed significant differences in expression of three chemosensory genes, namely two male-biased odorant-binding proteins and one male-biased odorant receptor, suggesting their possible involvement in pheromone detection. Our data pave the way for improving the understanding of the molecular basis of chemosensory reception in Coccinellidae.

scholarly journals Large-scale identification of odorant-binding proteins and chemosensory proteins from expressed sequence tags in insects

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 632 ◽  
Author(s):  
Ya-Long Xu ◽  
Peng He ◽  
Lan Zhang ◽  
Shao-Qing Fang ◽  
Shuang-Lin Dong ◽  
...  
Keyword(s):  
Expressed Sequence Tags ◽  
Large Scale ◽  
Binding Proteins ◽  
Odorant Binding Proteins ◽  
Chemosensory Proteins ◽  
Odorant Binding ◽  
Expressed Sequence

Characterization of candidate odorant-binding proteins and chemosensory proteins in the tea geometridEctropis obliquaProut (Lepidoptera: Geometridae)

2017 ◽  
Vol 94 (4) ◽  
pp. e21383 ◽  
Author(s):  
Liang Sun ◽  
Teng-Fei Mao ◽  
Yu-Xing Zhang ◽  
Jian-Jian Wu ◽  
Jia-He Bai ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Odorant Binding Proteins ◽  
Chemosensory Proteins ◽  
Odorant Binding

scholarly journals Identification and Expression Profiling of Peripheral Olfactory Genes in the Parasitoid Wasp Aphidius ervi (Hymenoptera: Braconidae) Reared on Different Aphid Hosts

Insects ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 397
Author(s):  
Gabriel I. Ballesteros ◽  
Daniela A. Sepúlveda ◽  
Christian C. Figueroa
Keyword(s):  
Binding Proteins ◽  
Host Preference ◽  
Odorant Receptors ◽  
Aphidius Ervi ◽  
Host Acceptance ◽  
Odorant Binding Proteins ◽  
Expression Levels ◽  
Host Fidelity ◽  
Chemosensory Genes ◽  
Odorant Binding

Generalist parasitoids of aphids, such as the wasp Aphidius ervi, display significant differences in terms of host preference and host acceptance, depending on the host on which they developed (natal host), which is preferred over a non-natal host, a trait known as host fidelity. This trait allows females to quickly find hosts in heterogeneous environments, a process mediated by chemosensory/olfactory mechanisms, as parasitoids rely on olfaction and chemical cues during host selection. Thus, it is expected that proteins participating in chemosensory recognition, such as odorant-binding proteins (OBPs) and odorant receptors (ORs) would play a key role in host preference. In this study, we addressed the effect of parasitoid reciprocal host switching between two aphid hosts (Sitobion avenae and Acyrthosiphon pisum) on the expression patterns of chemosensory genes in the wasp A. ervi. First, by using a transcriptomic approach based on RNAseq of A. ervi females reared on S. avenae and A. pisum, we were able to annotate a total of 91 transcripts related to chemoperception. We also performed an in-silico expression analysis and found three OBPs and five ORs displaying different expression levels. Then, by using qRT-PCR amplification, we found significant differences in the expression levels of these eight genes when the parasitoids were reciprocally transplanted from S. avenae onto A. pisum and vice versa. This suggests that the expression levels of genes coding for odorant receptors and odorant-binding proteins would be regulated by the specific plant–aphid host complex where the parasitoids develop (maternal previous experience) and that chemosensory genes coding for olfactory mechanisms would play a crucial role on host preference and host acceptance, ultimately leading to the establishment of host fidelity in A. ervi parasitoids.

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