scholarly journals Peer Review #2 of "Sex- and tissue-specific expression of odorant-binding proteins and chemosensory proteins in adults of the scarab beetle Hylamorpha elegans (Burmeister) (Coleoptera: Scarabaeidae) (v0.1)"

2019 ◽  
Keyword(s):  
Peer Review ◽  
Binding Proteins ◽  
Scarab Beetle ◽  
Specific Expression ◽  
Tissue Specific ◽  
Odorant Binding Proteins ◽  
Chemosensory Proteins ◽  
Tissue Specific Expression ◽  
Odorant Binding

scholarly journals Sex- and tissue-specific expression of odorant-binding proteins and chemosensory proteins in adults of the scarab beetle Hylamorpha elegans (Burmeister) (Coleoptera: Scarabaeidae)

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7054 ◽  
Author(s):  
Angélica González-González ◽  
María E. Rubio-Meléndez ◽  
Gabriel I. Ballesteros ◽  
Claudio C. Ramírez ◽  
Rubén Palma-Millanao
Keyword(s):  
Binding Proteins ◽  
Expression Patterns ◽  
Scarab Beetle ◽  
Control Methods ◽  
Specific Expression ◽  
Odorant Binding Proteins ◽  
Chemosensory Proteins ◽  
Qrt Pcr ◽  
Odorant Binding ◽  
Pest Control Methods

In this study, we addressed the sex- and tissue-specific expression patterns of odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) in Hylamorpha elegans (Burmeister), an important native scarab beetle pest species from Chile. Similar to other members of its family, this scarab beetle exhibit habits that make difficult to control the pest by conventional methods. Hence, alternative ways to manage the pest populations based on chemical communication and signaling (such as disrupting mating or host finding process) are highly desirable. However, developing pest-control methods based on chemical communication requires to understand the molecular basis for pheromone recognition/chemical perception in this species. Thus, with the aim of discovering olfaction-related genes, we obtained the first reference transcriptome assembly of H. elegans. We used different tissues of adult beetles from males and females: antennae and maxillary palps, which are well known for embedded sensory organs. Then, the expression of predicted odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) was analyzed by qRT-PCR. In total, 165 transcripts related to chemoperception were predicted. Of these, 16 OBPs, including one pheromone-binding protein (PBP), and four CSPs were successfully amplified by qRT-PCR. All of these genes were differentially expressed in the sensory tissues with respect to the tibial tissue that was used as a control. The single predicted PBP found was highly expressed in the antennal tissues, particularly in males, while several OBPs and one CSP showed male-biased expression patterns, suggesting that these proteins may participate in sexual recognition process. In addition, a single CSP was expressed at higher levels in female palps than in any other studied condition, suggesting that this CSP would participate in oviposition process. Finally, all four CSPs exhibited palp-biased expression while mixed results were obtained for the expression of the OBPs, which were more abundant in the palps than in the antennae. These results suggest that these chemoperception proteins would be interesting novel targets for control of H. elegans, thus providing a theoretical basis for further studies involving new pest control methods.

scholarly journals Large-scale identification of odorant-binding proteins and chemosensory proteins from expressed sequence tags in insects

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 632 ◽  
Author(s):  
Ya-Long Xu ◽  
Peng He ◽  
Lan Zhang ◽  
Shao-Qing Fang ◽  
Shuang-Lin Dong ◽  
...  
Keyword(s):  
Expressed Sequence Tags ◽  
Large Scale ◽  
Binding Proteins ◽  
Odorant Binding Proteins ◽  
Chemosensory Proteins ◽  
Odorant Binding ◽  
Expressed Sequence

Identification and analysis of chemosensory genes encoding odorant-binding proteins, chemosensory proteins and sensory neuron membrane proteins in the antennae of Lissorhoptrus oryzophilus

2021 ◽  
pp. 1-11
Author(s):  
Yu Pan ◽  
Xinxin Zhang ◽  
Zhun Wang ◽  
Lizhong Qi ◽  
Xinsheng Zhang ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Sensory Neuron ◽  
Binding Proteins ◽  
Mate Location ◽  
Lissorhoptrus Oryzophilus ◽  
Neuron Membrane ◽  
Odorant Binding Proteins ◽  
Chemosensory Proteins ◽  
Chemosensory Genes ◽  
Odorant Binding

Abstract The rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), is a destructive pest that causes damage to rice crops worldwide. The olfactory system is critical for host or mate location by weevils, but only limited information about the molecular mechanism of olfaction-related behaviour has been reported in this insect. In this study, we conducted SMRT-seq transcriptome analysis and obtained 54,378 transcripts, 38,706 of which were annotated. Based on these annotations, we identified 40 candidate chemosensory genes, including 31 odorant-binding proteins (OBPs), six chemosensory proteins (CSPs) and three sensory neuron membrane proteins (SNMPs). Phylogenetic analysis showed that LoryOBPs, LoryCSPs and LorySNMPs were distributed in various clades. The results of tissue expression patterns indicated that LoryOBPs were highly abundant in the antennae, whereas LoryCSPs were highly abundant not only in the antennae but also in the abdomen, head and wings. Our findings substantially expand the gene database of L. oryzophilus and may serve as a basis for identifying novel targets to disrupt key olfactory genes, potentially providing an eco-friendly strategy to control this pest in the future.

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