Immunoprecipitins in human schistosomiasis detected with adult worm antigens released by 3M KC1

1977 ◽  
Vol 51 (1) ◽  
pp. 71-72 ◽  
Author(s):  
Mauro Scapin ◽  
Miriam Tendler

Several methods are being applied for solubilizing adult Schistosoma mansoni antigens which may allow their immunochemical characterization and purification. Studies on antigens and immunoprecipitins in schistosome infections have been carried out with homogenized extracts of adult worms and/or larval forms of Schistosoma mansoni in saline (Biguet et al., 1962; Kagan and Norman, 1963; Silva and Ferri, 1965), or water (Kent, 1963). Kusel (1972) studied surface proteins in S. mansoni membrane, treating the worms with 0·5% saponin in 3% calcium chloride. Murrel et al. (1974) compared by immunodiffusion a crude culture antigen with extracts obtained by freeze-thawing of adult worms and by treatment in 3M KCl.

2009 ◽  
Vol 83 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Y. Mitsui ◽  
M. Miura ◽  
Y. Aoki

AbstractThe effect of artesunate (ART) on the survival time of adult worm pairs of Schistosoma mansoni and on their egg output during in vitro culture was assessed. ART significantly decreased the survival time of both paired male and female worms at concentrations of 5, 10, 20 and 40 mg l− 1 during in vitro cultivation. An inhibitory effect of ART on the daily egg output of paired female worms during in vitro cultivation was also observed.


2001 ◽  
Vol 96 (suppl) ◽  
pp. 79-83 ◽  
Author(s):  
Nilton Thaumaturgo ◽  
Mônica Magno Vilar ◽  
Catia Maria Diogo ◽  
Ricardo Edelenyi ◽  
Miriam Tendler

1997 ◽  
Vol 19 (7) ◽  
pp. 333-335 ◽  
Author(s):  
M. WEBSTER ◽  
P.G. FALLON ◽  
A.J.C. FULFORD ◽  
A.E. BUTTERWORTH ◽  
J.H. OUMA ◽  
...  

2002 ◽  
Vol 35 (3) ◽  
pp. 377-381 ◽  
Author(s):  
C.F.A. Brito ◽  
G.C. Oliveira ◽  
S.C. Oliveira ◽  
M. Street ◽  
S. Riengrojpitak ◽  
...  

1992 ◽  
Vol 34 (3) ◽  
pp. 205-209 ◽  
Author(s):  
Alan L. Melo ◽  
Leógenes H. Pereira ◽  
Conceição R. S. Machado

The peritoneal cavity of laboratory mice was used to study the phenomenon of host cell adhesion to different evolutive stages of the Schistosoma mansoni (cercaria, adult worm, developing and mature eggs, miracidium, young and mature daughter sporocysts). Material recovered from the peritoneal cavity 30 and 180 min after the inoculation of each evolutive form was examined with the help of a stereomicroscope. The free swimming larvae (cercaria and miracidium), and the evolutive forms producing such larvae (mature egg and mature daughter sporocyst) elicited the host cell adhesion phenomenon. In all forms but cercariae the adherent cells remained as so till 180 minutes after inoculation


2014 ◽  
Vol 8 (3) ◽  
pp. e2745 ◽  
Author(s):  
Fernanda Ludolf ◽  
Paola R. Patrocínio ◽  
Rodrigo Corrêa-Oliveira ◽  
Andréa Gazzinelli ◽  
Franco H. Falcone ◽  
...  

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