scholarly journals In vitromethod for safety testing of foot-and-mouth disease vaccines

1970 ◽  
Vol 68 (2) ◽  
pp. 159-172 ◽  
Author(s):  
E. C. Anderson ◽  
P. B. Capstick ◽  
G. N. Mowat ◽  
F. B. Leech
Keyword(s):  
Tissue Culture ◽  
Culture System ◽  
Cell Sheet ◽  
Foot And Mouth Disease ◽  
Kidney Cells ◽  
Live Virus ◽  
Safety Testing ◽  
Safety Test ◽  
Antigen Present ◽  
Baby Hamster Kidney Cells

SummaryThe susceptibility of the tissue culture system to small amounts of residual live virus was not influenced by the inactivated antigen present. The depth of inoculum over the cell sheet did not affect results. Negative cultures frequently gave positive first (but not second or later) sub-cultures.Baby hamster kidney cells were always more sensitive than cattle tongues to infection with any of the strains used.Confidence in the safety test depends on the number of vaccination doses used; the tissue culture test can be made much more reliable than the cattle test because it is not limited to the 15 ml. of inoculum that restricts the cattle test.

scholarly journals Verification with the utility of an established rapid assessment of brain safety for newly developed vaccines

2019 ◽  
Vol 35 (1) ◽  
Author(s):  
Gwang-Ho Kim ◽  
Sun Shin Yi
Keyword(s):  
Infectious Diseases ◽  
Rapid Assessment ◽  
Foot And Mouth Disease ◽  
Vaccine Safety ◽  
International Standards ◽  
First Century ◽  
Safety Testing ◽  
Live Vaccines ◽  
Safety Test ◽  
The Brain

AbstractIn the twenty-first century, high contagious infectious diseases such as SARS (Severe Acute Respiratory Syndrome), MERS (Middle East Respiratory Syndrome), FMD (Foot-and-Mouth Disease) and AI (Avian Influenza) have become very prevalent, causing treat harm to humans and animals in aspect of public health, and economical issues. The critical problem is that newly-reported infectious diseases that humans firstly experience are expected to continue to emerge, and these diseases will be spreading out rapidly. Therefore, rapid and safe supplies of effective vaccines are most pivotal to prevent the rapid prevalent of new infection, but international standards or assessing protocol the safety of urgent vaccines are not established well. In our previous study, since we established a module to assess the brain safety of urgent vaccines, therefore, it is necessary to verify that this established module for assessing brain safety could work effectively in commercially available two vaccines (one killed- and on live-vaccines). We compared the results of Evans blue (EB) assay and qPCR analysis by injection of two kinds of vaccines, PBS and Lipopolysaccharide (LPS) under the condition of the module previously reported. We confirmed that the brain safety test module for urgent vaccine we established is very reproducible. Therefore, it is believed that this vaccine safety testing method can be used to validate brain safety when prompt supply of a newly developed vaccines is needed.

IMPROVEMENTS IN THE TISSUE CULTURE SAFETY TESTING OF SALK VACCINES

1959 ◽  
Vol 5 (5) ◽  
pp. 453-459 ◽  
Author(s):  
R. C. French ◽  
R. E. Armstrong ◽  
W. Yarosh
Keyword(s):  
Tissue Culture ◽  
Volume Of Fluid ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Routine Test ◽  
Test Volume ◽  
Polio Virus ◽  
Safety Testing ◽  
First Order ◽  
Modified Test

Under the conditions of the official Canadian test, it was found that two simultaneous first-order reactions were in competition. Thus, after 4 hours, while 15% of any initial live MEF1 poliovirus present was adsorbed to the monkey kidney cells, 9% of the virus was destroyed by spontaneous inactivation. When the adsorption rate was increased by decreasing the volume of fluid, it was found that significantly more isolations of virus were obtained. Utilizing the method of Baron for the concentration of polio virus it was found possible to maintain a low test volume without making the routine test unduly cumbersome. The modified test is simpler and more sensitive and may be used with undialyzed vaccines.

Sign in / Sign up

Export Citation Format

Share Document