The application of RAPD markers to the study of the bivalve mollusc Lasaea rubra

This paper describes the random amplification of polymorphic DNA markers (RAPDs) in Lasaea rubra (Erycinidae: Bivalvia). Present evidence suggests that L. rubra is an asexual species; however, the exact mode of clonal reproduction in this species is still a matter of debate. In this preliminary study, four of the primers used generated polymorphic RAPDs. One primer was able to distinguish between individuals from the same or different crevice population. This same primer also resolved a single band difference between otherwise identical RAPD patterns of a parent and its offspring. No familial differences have been detected in several previous studies using allozyme electrophoresis. This paper suggests that many polymorphic markers could be obtained with this species using the RAPD technique. Population genetic analysis of L. rubra has long been hampered by a dearth of polymorphic markers due to its small size. These findings suggest that this technique has the potential to further the study of population genetics in this asexual species.

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Application of DNA Markers for Population Genetic Analysis

Aquaculture Genome Technologies ◽

10.1002/9780470277560.ch9 ◽

2007 ◽

pp. 109-137 ◽

Cited By ~ 3

Author(s):

Eric M. Hallerman ◽

Paul J. Grobler ◽

Jess W. Jones

Keyword(s):

Genetic Analysis ◽

Dna Markers ◽

Population Genetic ◽

Population Genetic Analysis

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Population genetic analysis of European Prunus spinosa (Rosaceae) using chloroplast DNA markers

American Journal of Botany ◽

10.3732/ajb.89.8.1223 ◽

2002 ◽

Vol 89 (8) ◽

pp. 1223-1228 ◽

Cited By ~ 31

Author(s):

A. Mohanty ◽

J. P. Martin ◽

I. Aguinagalde

Keyword(s):

Genetic Analysis ◽

Chloroplast Dna ◽

Dna Markers ◽

Population Genetic ◽

Population Genetic Analysis ◽

Prunus Spinosa ◽

Chloroplast Dna Markers

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Identification of Grape (Vitis) Rootstocks Using Sequence Characterized Amplified Region DNA Markers

HortScience ◽

10.21273/hortsci.31.2.267 ◽

1996 ◽

Vol 31 (2) ◽

pp. 267-268 ◽

Cited By ~ 10

Author(s):

Hong Xu ◽

Alan T. Bakalinsky

Keyword(s):

Dna Markers ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Scar Markers ◽

Polymorphic Markers ◽

Sequence Characterized Amplified Region ◽

Annealing Temperatures

Five sequence characterized amplified region (SCAR) DNA markers were reevaluated at substantially higher annealing temperatures than originally reported; four were polymorphic among nine rootstocks tested. Four new informative SCAR markers also are reported, based on redesigning primers from previously cloned random amplified polymorphic DNA (RAPD) markers. Based on the eight polymorphic markers, rootstocks MG 420A, MG101-14, Richter 99, Couderc 3309, and Kober 5BB were distinguishable. Riparia Gloire and Couderc 1616 could be distinguished from the others, but not from one another, and SO4 and 5C also could be distinguished from the others, but not from one another.

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Preliminary Study of Population Genetic Diversity of Hyalinoecia tubicola (Polychaeta: Onuphidae) from the North East Coast of Tunisia (Western Mediterranean) using Random Amplified Polymorphic DNA Markers

Journal of Coastal Zone Management ◽

10.4172/2473-3350.1000397 ◽

2015 ◽

Vol 18 (1) ◽

Cited By ~ 1

Author(s):

Sana Zaabi ◽

Isabelle Metais

Keyword(s):

Genetic Diversity ◽

Dna Markers ◽

Population Genetic ◽

East Coast ◽

Random Amplified Polymorphic Dna ◽

Western Mediterranean ◽

Population Genetic Diversity ◽

North East ◽

The North ◽

Preliminary Study

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Random Amplified Polymorphic Markers as Indicator for Genetic Conservation Program in Iranian Pheasant (Phasianus colchicus)

The Scientific World JOURNAL ◽

10.1100/2012/640381 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

Ghorban Elyasi Zarringhabaie ◽

Arash Javanmard ◽

Ommolbanin Pirahary

Keyword(s):

Dna Markers ◽

Genetic Similarity ◽

Rapd Markers ◽

Conservation Strategy ◽

Phasianus Colchicus ◽

Polymorphic Markers ◽

Genetic Origin ◽

Arbitrary Primers ◽

Conservation Program ◽

Polymorphic Bands

The objective of present study was identification of genetic similarity between wild Iran and captive Azerbaijan Pheasant using PCR-RAPD markers. For this purpose, in overall, 28 birds were taken for DNA extraction and subsequently 15 arbitrary primers were applied for PCR-RAPD technique. After electrophoresis, five primers exhibited sufficient variability which yielded overall 65 distinct bands, 59 polymorphic bands, for detalis, range of number of bands per primer was 10 to 14, and produced size varied between 200 to 1500 bp. Highest and lowest polymorphic primers were OPC5, OPC16 (100%) and OPC15 (81%), respectively. Result of genetic variation between two groups was accounted as nonsignificant (8.12%) of the overall variation. According to our expectation the wild Iranian birds showed higher genetic diversity value than the Azerbaijan captive birds. As general conclusion, two pheasant populations have almost same genetic origin and probably are subpopulations of one population. The data reported herein could open the opportunity to search for suitable conservation strategy to improve richness of Iran biodiversity and present study here was the first report that might have significant impact on the breeding and conservation program of Iranian pheasant gene pool. Analyses using more regions, more birds, and more DNA markers will be useful to confirm or to reject these findings.

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Adaptive Evolution of Relish, a Drosophila NF-κB/IκB Protein

Genetics ◽

10.1093/genetics/154.3.1231 ◽

2000 ◽

Vol 154 (3) ◽

pp. 1231-1238 ◽

Cited By ~ 1

Author(s):

David J Begun ◽

Penn Whitley

Keyword(s):

Immune Response ◽

Innate Immunity ◽

Genetic Analysis ◽

Adaptive Evolution ◽

Strong Evidence ◽

Population Genetic ◽

Microbial Pathogens ◽

Population Genetic Analysis ◽

Evolutionary Conflict ◽

A Site

Abstract NF-κB and IκB proteins have central roles in regulation of inflammation and innate immunity in mammals. Homologues of these proteins also play an important role in regulation of the Drosophila immune response. Here we present a molecular population genetic analysis of Relish, a Drosophila NF-κB/IκB protein, in Drosophila simulans and D. melanogaster. We find strong evidence for adaptive protein evolution in D. simulans, but not in D. melanogaster. The adaptive evolution appears to be restricted to the IκB domain. A possible explanation for these results is that Relish is a site of evolutionary conflict between flies and their microbial pathogens.

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