Specific cross-immunity between Trichinella spiralis and Trichuris muris: immunization with heterologous infections and antigens and transfer of immunity with heterologous immune mesenteric lymph node cells

Parasitology ◽  
1982 ◽  
Vol 84 (2) ◽  
pp. 381-389 ◽  
Author(s):  
T. D. G. Lee ◽  
R. K. Grencis ◽  
D. Wakelin
Keyword(s):  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Primary Infection ◽  
Trichinella Spiralis ◽  
The Other ◽  
Trichuris Muris ◽  
Mesenteric Lymph ◽  
Heterologous Challenge ◽  
Lymph Node Cells ◽  
Cross Immunity

SUMMARYInfections with either 300 infective Trichinella spiralis larvae or 400 embryonated eggs of Trichuris muris were effective in eliciting accelerated expulsion of heterologous challenge infections given 20 days after the primary infection. Accelerated expulsion could also be achieved by the administration of soluble crude worm antigen given 12 days prior to heterologous challenge or by adoptive transfer of mesenteric lymph node cells taken from mice infected with the heterologous parasite. Each species is capable of eliciting an accelerated secondary expulsion response in hosts that have been actively or adoptively immunized against the other species and these results are taken to indicate that there is a specific cross-immunity between T. spiralis and T. muris due to shared antigens. It is postulated that these shared antigens are derived from stichocyte granules.

Immune expulsion of Trichuris muris from mice during a primary infection: analysis of the components involved

Parasitology ◽  
1975 ◽  
Vol 70 (3) ◽  
pp. 397-405 ◽  
Author(s):  
D. Wakelin
Keyword(s):  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Primary Infection ◽  
Immune Serum ◽  
Trichuris Muris ◽  
Mesenteric Lymph ◽  
Serum Transfer ◽  
Lymph Node Cells ◽  
Worm Expulsion ◽  
Sublethal Irradiation

Immune serum accelerated the expulsion of Trichuris muris when transferred into normal mice on days 0 and 3 after infection, but had no effect when the recipient mice had been immunosuppressed by sublethal irradiation or by cortisone treatment. Delaying serum transfer until days 7 and 8 in normal mice failed to accelerate expulsion, although immune mesenteric lymph node cells (MLNC) accelerated expulsion whether transferred early or late in infection.Expulsion from NIH mice, normally complete by 12 days, was prevented by sublethal irradiation given as late as 9 days after infection, but could be restored by subsequent transfer of immune MLNC or, to a lesser degree, non-immune MLNC. Immune MLNC were unable to restore worm expulsion in mice irradiated before infection.These results are interpreted as showing that the immune expulsion of T. muris from mice during a primary infection requires the sequential activities of antibody-mediated and lymphoid cell-mediated components.

Evidence for the involvement of a bone marrow-derived cell population in the immune expulsion ofTrichinella spiralis

Parasitology ◽  
1977 ◽  
Vol 74 (3) ◽  
pp. 225-234 ◽  
Author(s):  
D. Wakelin ◽  
Margaret M. Wilson
Keyword(s):  
Bone Marrow ◽  
Lymph Node ◽  
Cell Population ◽  
Mesenteric Lymph Node ◽  
Trichinella Spiralis ◽  
Mesenteric Lymph ◽  
Lymph Node Cells

When mice were irradiated immediately before infection withTrichinella spiralisthere was a profound and long-lasting interference with their ability to expel adult worms from the intestine. Irradiation given after the fifth day of infection was progressively less effective in this respect. The ability to expel worms was not restored when mesenteric lymph node cells (MLNC) were transferred (a) on the day of infection in mice irradiated one day previously, or (b) on day 7 of an infection in mice irradiated on day 6, even though the MLNC transferred immunity to intact recipients. Transfer of bone marrow (BM) alone was also without effect. However, worm explusion was restored if, following irradiation and injection of BM, 10 days were allowed for BM differentiation before transfer of MLNC. This restoration was effective even after lethal levels of irradiation and was clearly dependent upon a donor-derived BM component cooperating with, or responding to, the activity of the transferred MLNC. The possibility that the BM component is non-lymphoid in nature is discussed.

Accelerated expulsion of adult Trichinella spiralis in mice given lymphoid cells and serum from infected donors

Parasitology ◽  
1976 ◽  
Vol 72 (3) ◽  
pp. 307-315 ◽  
Author(s):  
D. Wakelin ◽  
M. Lloyd
Keyword(s):  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Adult Stage ◽  
Trichinella Spiralis ◽  
Significant Degree ◽  
Lymphoid Cells ◽  
Mesenteric Lymph ◽  
Lymph Node Cells ◽  
Worm Expulsion

SummaryImmunity to the adult stage of Trichinella spiralis, assessed by an acceleration of worm expulsion, was transferred to recipient mice with mesenteric lymph node cells (MLNC) or serum taken from infected donors. Immunity was transferred most effectively by MLNC taken from donors infected for 8 days, i.e. donors actively responding to infection. Transfer of both MLNC and serum brought about a marked acceleration of worm expulsion in all cases, even where MLNC or serum given separately failed to transfer a significant degree of immunity.

Transfer of immunity toTrichinella spiralisin the mouse with mesenteric lymph node cells: time of appearance of effective cells in donors and expression of immunity in recipients

Parasitology ◽  
1977 ◽  
Vol 74 (3) ◽  
pp. 215-224 ◽  
Author(s):  
D. Wakelin ◽  
Margaret M. Wilson
Keyword(s):  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Time Course ◽  
Trichinella Spiralis ◽  
Defence Mechanism ◽  
Spleen Cells ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Lymph Node Cells ◽  
Worm Expulsion

Cells capable of transferring immunity toTrichinella spiralis, i.e. of accelerating adult worm expulsion, were present in the mesenteric lymph nodes of mice infected for 4, 6 or 8 days, but not in mice infected for only 2 days. The time-course of worm expulsion in mice infected on the day of transfer was similar in recipients of day 8 cells, expulsion becoming marked only when the recipients had been infected for at least 6 days. Transfer of cells 4 or 6 days after infection did not result in an accelerated worm expulsion; transfer 1 or 2 weeks before infection did not enhance the level of immunity in recipient mice. In contrast to the results obtained with mesenteric lymph node cells (MLNC) no immunity was trsnsferred when recipients were given spleen cells taken from donors infected for 8 days. It is suggested that MLNC do not cause worm expulsion directly, but cooperate with another component of the host's defence mechanism. Accelerated expulsion in recipients of cells was accompanied by a premature decline in fecundity of female worms. Evidence is presented to show that worm expulsion and impaired reproduction may represent independent aspects of the immune response toT. spiralis.

Dietary effects on insulin and nutrient metabolism in mesenteric lymph node cells, splenocytes, and pancreatic islets of BB rats

Metabolism ◽  
2000 ◽  
Vol 49 (9) ◽  
pp. 1111-1117 ◽  
Author(s):  
Fraser W. Scott ◽  
Elizabeth Olivares ◽  
Abdullah Sener ◽  
Willy J. Malaisse
Keyword(s):  
Lymph Node ◽  
Pancreatic Islets ◽  
Mesenteric Lymph Node ◽  
Nutrient Metabolism ◽  
Dietary Effects ◽  
Mesenteric Lymph ◽  
Bb Rats ◽  
Lymph Node Cells

scholarly journals Adoptive transfer of nontransgenic mesenteric lymph node cells induces colitis in athymic HLA-B27 transgenic nude rats

2006 ◽  
Vol 143 (3) ◽  
pp. 474-483 ◽  
Author(s):  
F. Hoentjen ◽  
S. L. Tonkonogy ◽  
B. Liu ◽  
R. B. Sartor ◽  
J. D. Taurog ◽  
...  
Keyword(s):  
Lymph Node ◽  
Adoptive Transfer ◽  
Mesenteric Lymph Node ◽  
Hla B27 ◽  
Mesenteric Lymph ◽  
Nude Rats ◽  
Lymph Node Cells

scholarly journals Recombinant Proteins of Cryptosporidium parvum Induce Proliferation of Mesenteric Lymph Node Cells in Infected Mice

2005 ◽  
Vol 73 (8) ◽  
pp. 5245-5248 ◽  
Author(s):  
Inderpal Singh ◽  
Cynthia Theodos ◽  
Saul Tzipori
Keyword(s):  
Lymph Node ◽  
Immune Responses ◽  
Recombinant Proteins ◽  
Cryptosporidium Parvum ◽  
Mesenteric Lymph Node ◽  
Recombinant Antigens ◽  
Cellular Immune Responses ◽  
Mesenteric Lymph ◽  
Lymph Node Cells ◽  
Cellular Immune

ABSTRACT Recombinant antigens of Cryptosporidium parvum, Cp900 and Cp40 but not Cp15, stimulated C. parvum-specific proliferative immune responses of mesenteric lymph node cells in C57BL/6J mice infected with different isolates (MD, GCH1, UCP, and IOWA) of C. parvum, indicating that both Cp900 and Cp40 are immunodominant targets of cellular immune responses during C. parvum infection.

Sign in / Sign up

Export Citation Format

Share Document