Trypanosoma congolense: appearance and distribution of variable antigen types during metacyclic differentiation in vitro

SummaryDifferentiation of epimastigotes and production of infective metacyclic forms of Trypanosoma congolense were examined in a culture system which enabled manipulation of the population density of insect forms. Scanning electron microscopy of cultures revealed the attachment sites of epimastigotes in detail, showing them to be attached as ‘clusters’ or ‘bundles’ and having associated fibrillar structures. Dividing epimastigotes were observed either within individual bundles or in association with two bundles. Metacyclic forms were detected by an immunofluorescence antibody test (IFAT) using metacyclic variable-antigen type (M-VAT) specific monoclonal antibodies, by day 7 after seeding cultures. Trypanosomes expressing M-VATs appeared singly in bundles, observed by both IFAT and an immunogold labelling method. Statistical analysis using Poisson calculations suggested that, in general, the distribution of metacyclics expressing individual M-VATs was random throughout cultures.

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Diagnostic Techniques To Detect Cryptic Leishmaniasis in Dogs

Clinical and Vaccine Immunology ◽

10.1128/cdli.9.5.1137-1141.2002 ◽

2002 ◽

Vol 9 (5) ◽

pp. 1137-1141 ◽

Cited By ~ 3

Author(s):

Laura Iniesta ◽

Salceda Fernández-Barredo ◽

Béatrice Bulle ◽

M. Teresa Gómez ◽

Renaud Piarroux ◽

...

Keyword(s):

Lymphocyte Proliferation ◽

Antibody Test ◽

Diagnostic Techniques ◽

Delayed Type Hypersensitivity ◽

Diagnostic Tools ◽

Enzyme Linked Immunosorbent Assay ◽

Proliferation Assay ◽

Immunofluorescence Antibody Test ◽

Immunofluorescence Antibody

ABSTRACT This study of several techniques for detecting cryptic leishmaniasis in dogs from areas in Spain where Leishmania infantum is highly endemic concludes that immunological techniques (enzyme-linked immunosorbent assay, immunofluorescence antibody test, Western blotting, delayed-type hypersensitivity reaction, and in vitro lymphocyte proliferation assay) do not clearly differentiate between noninfected and infected asymptomatic dogs and that culture and PCR are more reliable diagnostic tools.

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Seroprevalence of Toxoplasma gondii in dogs of riverside communities of Mato Grosso Pantanal, Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612016067 ◽

2016 ◽

Vol 25 (4) ◽

pp. 531-535 ◽

Cited By ~ 7

Author(s):

Juliana Yuki Rodrigues ◽

Arleana do Bom Parto Ferreira de Almeida ◽

Eveline da Cruz Boa Sorte ◽

Naiani Domingos Gasparetto ◽

Felipe Augusto Constantino Seabra da Cruz ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Antibody Test ◽

Human Infection ◽

Mato Grosso ◽

Exact Test ◽

Worldwide Distribution ◽

Significant Difference ◽

Chi Squared ◽

Immunofluorescence Antibody Test ◽

Immunofluorescence Antibody

Abstract Toxoplasma gondii is an intracellular protozoan with worldwide distribution and dogs act as sentinels of human infection. This search aimed to determine the occurrence of antibodies against T. gondii in dogs of the communities on the Cuiabá River, Mato Grosso and variables associated with infection. The dogs of the riverside communities in Cuiabá River, which includes Barranco Alto, Praia do Poço, Engenho Velho, Varginha, Bom Sucesso, Passagem da Conceição and São Gonçalo Beira Rio, were evaluated for the presence of T. gondii antibodies by indirect immunofluorescence antibody test (IFAT). The prevalence and factors associated with infection were calculated by chi-squared test (χ2) or Fisher’s exact test, and univariate and multiple analysis. Of the 248 dogs surveyed, 107 (43.1%) were seropositive for T. gondii. The seroprevalence ranged from 25.6% to 64.3%. There was no statistically significant difference between the communities studied (p > 0.05). As for the associated factors, the only statistically significant factor was that of dogs living with cats (p = 0.02), with approximately twice the risk of acquiring infection. In conclusion, the seroprevalence in dogs of riverside communities in the Baixada Cuiabana demonstrated that high rates of infection, being the factor associated with infection, contact with domestic cats.

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The immunofluorescence antibody test (IFAT) for the diagnosis of schistosomiasis used in a non-endemic area

Tropical Medicine & International Health ◽

10.1046/j.1365-3156.2000.00539.x ◽

2000 ◽

Vol 5 (3) ◽

pp. 185-191 ◽

Cited By ~ 25

Author(s):

Britta Tarp ◽

Finn Trunk Black ◽

Eskild Petersen

Keyword(s):

Endemic Area ◽

Antibody Test ◽

Immunofluorescence Antibody Test ◽

Immunofluorescence Antibody

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Immunofluorescence Antibody Test (IFAT)

Encyclopedia of Parasitology ◽

10.1007/978-3-662-43978-4_1588 ◽

2016 ◽

pp. 1332-1332

Keyword(s):

Antibody Test ◽

Immunofluorescence Antibody Test ◽

Immunofluorescence Antibody

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Low prevalence of infection by Sarcocystis neurona in horses from the State of Alagoas, Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612019027 ◽

2019 ◽

Vol 28 (2) ◽

pp. 298-302 ◽

Cited By ~ 1

Author(s):

Sandra Regina Fonseca de Araújo Valença ◽

Müller Ribeiro-Andrade ◽

Gastón Moré ◽

Pedro Paulo Feitosa de Albuquerque ◽

José Wilton Pinheiro Júnior ◽

...

Keyword(s):

Risk Factors ◽

Antibody Test ◽

Sarcocystis Neurona ◽

Animal Management ◽

Serological Status ◽

Potential Risk Factors ◽

Low Exposure ◽

Low Prevalence ◽

Immunofluorescence Antibody Test ◽

Immunofluorescence Antibody

Abstract The aim of this study was to determine the prevalence of infection by Sarcocystis neurona in horses and identify potential risk factors. Were analyzed 427 samples from 36 farms in 21 municipalities in the Alagoas State, Brazil. Presence of anti-S. neurona antibodies was diagnosed by indirect immunofluorescence antibody test (IFAT) and was confirmed using the immunoblot test. Risk factors were assessed through investigative questionnaires on animal management on the farms. The prevalence of anti-S.neurona antibodies was 2.8% (confidence interval, CI: 1.5-4.9%) from IFAT and 1.6% (CI:0.8-3.34%) from immunoblot, and there were positive horses on 16.6% of the studied farms. None of the variables studied presented associations with serological status for S. neurona. This is the first report on infection by S. neurona in horses reared in Alagoas, Brazil showing a low exposure to S. neurona in this region, but with significant numbers of foci.

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An indirect immunofluorescence antibody test employing whole eggs as the antigen for the diagnosis of abdominal angiostrongyliasis

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02762011000400002 ◽

2011 ◽

Vol 106 (4) ◽

pp. 390-393 ◽

Cited By ~ 4

Author(s):

Elizabeth Abrahams-Sandi ◽

Paolo Mesén-Ramírez ◽

Dimelsa Suarez-Chacón ◽

Katherine Fernández-Quesada

Keyword(s):

Indirect Immunofluorescence ◽

Antibody Test ◽

Immunofluorescence Antibody Test ◽

Immunofluorescence Antibody

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Giardia sp. and Cryptosporidium sp. in Iberian Wolf

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.20864 ◽

2019 ◽

Vol 70 (2) ◽

pp. 1579

Author(s):

A.L. PEREIRA ◽

T. L. MATEUS ◽

L. LLANEZ ◽

S. D. DUARTE

Keyword(s):

Canis Lupus ◽

Antibody Test ◽

Direct Immunofluorescence ◽

Fecal Samples ◽

First Report ◽

Immunofluorescence Antibody Test ◽

Immunofluorescence Antibody ◽

Canis Lupus Signatus ◽

Iberian Wolf

A subsample consisting of fifty fecal samples from wild Iberian Wolf (Canis lupus signatus), from the northwest of Spain were collected in the field. The samples were analyzed for cysts of Giardia sp. and oocysts of Cryptosporidium sp. using a direct immunofluorescence antibody test (IFA). Giardia sp. and Cryptosporidium sp. were found in 20.0 % of the samples examined. Simple infections were more frequent (90.0 %) with seven (14.0 %) positive for Giardia sp. and two (4.0 %) positive for Cryptosporidium sp. To the authors’ knowledge, this is the first report of occurrence of Cryptosporidium sp. in Iberian Wolf.

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Occurrences of anti-Toxoplasma gondiiand anti-Neospora caninum antibodies in Barbary sheep at Curitiba zoo, southern Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612014034 ◽

2014 ◽

Vol 23 (2) ◽

pp. 255-259 ◽

Cited By ~ 4

Author(s):

Vivien Midori Morikawa ◽

Cristina Kraemer Zimpel ◽

Igor Adolfo Dexheimer Paploski ◽

Maria do Carmo Custódio de Souza Hunold Lara ◽

Eliana Monteforte Cassaro Villalobos ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Neospora Caninum ◽

Antibody Test ◽

Southern Brazil ◽

Intermediate Hosts ◽

Blood Samples ◽

Ammotragus Lervia ◽

Barbary Sheep ◽

Immunofluorescence Antibody Test ◽

Immunofluorescence Antibody

Barbary sheep (Ammotragus lervia) have the potential to act as hosts of important infectious diseases, particularly zoonoses. Blood samples from 17 Barbary sheep at the Curitiba zoo were collected to evaluate occurrences of anti-Toxoplasma gondii and anti-Neospora caninum antibodies, tested using the indirect immunofluorescence antibody test (IFAT). Anti-T. gondii and anti-N. caninum antibodies were detected in 4/17 (23.5%) and 4/17 (23.5%) samples, respectively. The present study has shown that Barbary sheep at Curitiba zoo were exposed to T. gondii andN. caninum and therefore may act as intermediate hosts, spreading toxoplasmosis and neosporosis within and between species in shared areas.

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