The TcTASV proteins are novel promising antigens to detect activeTrypanosoma cruziinfection in dogs

Parasitology ◽  
2016 ◽  
Vol 143 (11) ◽  
pp. 1382-1389 ◽  
Author(s):  
N. FLORIDIA-YAPUR ◽  
M. MONJE RUMI ◽  
P. RAGONE ◽  
J. J. LAUTHIER ◽  
N. TOMASINI ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Antibody Response ◽  
Chagas Disease ◽  
Murine Model ◽  
High Impact ◽  
Differentially Expressed ◽  
The Novel ◽  
Active Infection ◽  
Recombinant Antigens ◽  
Highly Correlated

SUMMARYIn regions where Chagas disease is endemic, canineTrypanosoma cruziinfection is highly correlated with the risk of transmission of the parasite to humans. Herein we evaluated the novel TcTASV protein family (subfamilies A, B, C), differentially expressed in bloodstream trypomastigotes, for the detection of naturally infected dogs. A gene of each TcTASV subfamily was cloned and expressed. Indirect enzyme-linked immunosorbent assays (ELISA) were developed using recombinant antigens individually or mixed together. Our results showed that dogs with activeT. cruziinfection differentially reacted against the TcTASV-C subfamily. The use of both TcTASV-C plus TcTASV-A proteins (Mix A+C-ELISA) enhanced the reactivity of sera from dogs with active infection, detecting 94% of the evaluated samples. These findings agree with our previous observations, where the infected animals exhibited a quick anti-TcTASV-C antibody response, coincident with the beginning of parasitaemia, in a murine model of the disease. Results obtained in the present work prove that the Mix A+C-ELISA is a specific, simple and cheap technique to be applied in endemic areas in screening studies. The Mix A+C-ELISA could help to differentially detect canine hosts with active infection and therefore with high impact in the risk of transmission to humans.

scholarly journals Enantiomers of Nifurtimox Do Not Exhibit Stereoselective Anti-Trypanosoma cruzi Activity, Toxicity, or Pharmacokinetic Properties

2015 ◽  
Vol 59 (6) ◽  
pp. 3645-3647 ◽  
Author(s):  
Carolina B. Moraes ◽  
Karen L. White ◽  
Stéphanie Braillard ◽  
Catherine Perez ◽  
Junghyun Goo ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Murine Model ◽  
Therapeutic Benefit ◽  
Racemic Mixture ◽  
Content Type ◽  
Pharmacokinetic Properties

ABSTRACTWith the aim of improving the available drugs for the treatment of Chagas disease, individual enantiomers of nifurtimox were characterized. The results indicate that the enantiomers are equivalent in theirin vitroactivity against a panel ofTrypanosoma cruzistrains;in vivoefficacy in a murine model of Chagas disease;in vitrotoxicity and absorption, distribution, metabolism, and excretion characteristics; andin vivopharmacokinetic properties. There is unlikely to be any therapeutic benefit of an individual nifurtimox enantiomer over the racemic mixture.

scholarly journals IL-10 and IFN-γ gene expression in chronic Chagas disease patients after in vitro stimulation with recombinant antigens of Trypanosoma cruzi

Cytokine ◽  
2012 ◽  
Vol 58 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Adriene Siqueira de Melo ◽  
Virginia Maria Barros de Lorena ◽  
Suellen Carvalho de Moura Braz ◽  
Cássia Docena ◽  
Yara de Miranda Gomes
Keyword(s):  
Gene Expression ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Recombinant Antigens ◽  
Ifn Γ ◽  
Chronic Chagas Disease

Cytokine Levels in Serious Cardiopathy of Chagas Disease After In Vitro Stimulation with Recombinant Antigens from Trypanosoma cruzi

2010 ◽  
Vol 72 (6) ◽  
pp. 529-539 ◽  
Author(s):  
V. M. B. Lorena ◽  
I. M. B. Lorena ◽  
S. C. M. Braz ◽  
A. S. Melo ◽  
M. F. A. D. Melo ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Recombinant Antigens ◽  
Cytokine Levels

scholarly journals Computational mapping of the differentially expressed gene-lncRNA pairs present at the root nodule developmental stages of Arachis hypogaea

2019 ◽  
Author(s):  
Ahsan Z. Rizvi ◽  
Kalyani Dhusia
Keyword(s):  
Arachis Hypogaea ◽  
Developmental Stages ◽  
Root Nodules ◽  
Differentially Expressed Gene ◽  
Differentially Expressed ◽  
The Novel ◽  
Rna Seq ◽  
Genetic Features ◽  
Antisense Lncrnas ◽  
Highly Correlated

AbstractRNA-sequencing (RNA-seq) data analysis of the different stages of root nodules formation in peanut Arachis hypogaea investigate the genetic features. Genes related to the root nodules formations in this plant are extensively studied [1] [2] [3] [4] [5], but less information is present for their relations with long noncoding RNAs (lncRNAs). Bioinformatics techniques are utilised here to identify the novel lncRNAs present in the publically available RNA-seq data reported [6] for the different stages of root nodules formation in this plant. Highly correlated, significant, and Differentially Expressed (DE) gene-lncRNA pairs are also detected to understand the epigenetic control of lncRNA. These pairs are further differentiated between cis and trans antisense lncRNAs and lincRNAs based on their functions and positions from the genes. Obtained results are the catalogue for the highly correlated and significant DE gene-lncRNA pairs related to root nodules formation in A. hypogaea.

scholarly journals Critical Proinflammatory and Anti-Inflammatory Functions of Different Subsets of CD1d-Restricted Natural Killer T Cells during Trypanosoma cruzi Infection

2005 ◽  
Vol 73 (1) ◽  
pp. 181-192 ◽  
Author(s):  
Malcolm S. Duthie ◽  
Maria Kahn ◽  
Maria White ◽  
Raj P. Kapur ◽  
Stuart J. Kahn
Keyword(s):  
T Cells ◽  
Trypanosoma Cruzi ◽  
Antibody Response ◽  
Natural Killer ◽  
Chagas Disease ◽  
Inkt Cells ◽  
Activation Markers ◽  
Mild Phenotype ◽  
Anti Inflammatory ◽  
Natural Killer T

ABSTRACT Trypanosoma cruzi infects 15 to 20 million people in Latin America and causes Chagas disease, a chronic inflammatory disease with fatal cardiac and gastrointestinal sequelae. How the immune response causes Chagas disease is not clear, but during the persistent infection both proinflammatory and anti-inflammatory responses are critical. Natural killer T (NKT) cells have been shown to regulate immune responses during infections and autoimmune diseases. We report here that during acute T. cruzi infection NKT-cell subsets provide distinct functions. CD1d−/− mice, which lack both invariant NKT (iNKT) cells and variant NKT (vNKT) cells, develop a mild phenotype displaying an increase in spleen and liver mononuclear cells, anti-T. cruzi antibody response, and muscle inflammation. In contrast, Jα18−/− mice, which lack iNKT cells but have vNKT cells, develop a robust phenotype involving prominent spleen, liver, and skeletal muscle inflammatory infiltrates comprised of NK, dendritic, B and T cells. The inflammatory cells display activation markers; produce more gamma interferon, tumor necrosis factor alpha, and nitric oxide; and show a diminished antibody response. Strikingly, most Jα18−/− mice die. Thus, in response to the same infection, vNKT cells appear to augment a robust proinflammatory response, whereas the iNKT cells dampen this response, possibly by regulating vNKT cells.

An improved serodiagnostic test for Chagas' disease employing a mixture of Trypanosoma cruzi recombinant antigens

Transfusion ◽  
2003 ◽  
Vol 43 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Eufrosina S. Umezawa ◽  
Sueli F. Bastos ◽  
Jose R. Coura ◽  
Mariano J. Levin ◽  
Antonio Gonzalez ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Recombinant Antigens

scholarly journals Expression of co-stimulatory molecules CD80 and CD86 is altered in CD14 + HLA-DR + monocytes from patients with Chagas disease following induction by Trypanosoma cruzi recombinant antigens

2016 ◽  
Vol 49 (5) ◽  
pp. 632-636 ◽  
Author(s):  
Ana Karine de Araújo Soares ◽  
Patrícia Areias Feitosa Neves ◽  
Maria da Glória Aureliano de Melo Cavalcanti ◽  
Sílvia Martins Marinho ◽  
Wilson de Oliveira Júnior ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Recombinant Antigens ◽  
Hla Dr

scholarly journals Comparative Efficacies of TAK-187, a Long-Lasting Ergosterol Biosynthesis Inhibitor, and Benznidazole in Preventing Cardiac Damage in a Murine Model of Chagas' Disease

2005 ◽  
Vol 49 (4) ◽  
pp. 1556-1560 ◽  
Author(s):  
Milagros Corrales ◽  
Rubén Cardozo ◽  
María Asunción Segura ◽  
Julio A. Urbina ◽  
Miguel Angel Basombrío
Keyword(s):  
Trypanosoma Cruzi ◽  
Comparative Study ◽  
Chagas Disease ◽  
Murine Model ◽  
Ergosterol Biosynthesis ◽  
Cardiac Damage ◽  
Experimental Animals ◽  
Biosynthesis Inhibitor

ABSTRACT We carried out a comparative study of benznidazole and TAK-187, a long-lasting ergosterol biosynthesis inhibitor, with a murine model of Chagas' disease. The results indicated that TAK-187 was more effective than benznidazole in preventing Trypanosoma cruzi-induced cardiac damage in experimental animals.

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