A re-investigation of the behavioural effects of intracerebral injection in marmosets of cytopathic cerebrospinal fluid from patients with schizophrenia or neurological disease

1989 ◽  
Vol 19 (2) ◽  
pp. 325-329 ◽  
Author(s):  
H. F. Baker ◽  
R. M. Ridley ◽  
T. J. Crow ◽  
D. A. J. Tyrrell
Keyword(s):  
Cell Culture ◽  
Cerebrospinal Fluid ◽  
Neurological Disease ◽  
Cytopathic Effect ◽  
Fibroblast Cell ◽  
Intracerebral Injection ◽  
Common Marmosets ◽  
Behavioural Effects ◽  
Fibroblast Cell Culture ◽  
Embryonic Fibroblast

SynopsisIn experiments designed to investigate transmission, cerebrospinal fluid (CSF) from patients with schizophrenia or neurological disease (Huntington's disease) which had been found to induce a cytopathic effect (CPE) in human embryonic fibroblast cell culture was injected intracerebrally into common marmosets. Behavioural observations were made on the animals during a period of 2½ years prior to injection and for 2½ years after injection. In an earlier study (Baker et al. 1983 b) we found that animals injected with CPE + ve CSF became progressively more inactive when compared with those injected with non-cytopathic (CPE – ve) CSF from control patients. In the present study we were unable to replicate this finding. No difference in behaviour emerged between animals injected with control CSF and animals injected with CSF from schizophrenics or patients with neurological disease, nor between animals injected with CPE + ve CSF and animals injected with CPE – ve CSF. The numbers of offspring produced and surviving did not differ between the groups. We conclude that the original findings were due to factors unconnected with the nature of the injected material.

An investigation of the effects of intracerebral injection in the marmoset of cytopathic cerebrospinal fluid from patients with schizophrenia or neurological disease

1983 ◽  
Vol 13 (3) ◽  
pp. 499-511 ◽  
Author(s):  
H. F. Baker ◽  
R. M. Ridley ◽  
T. J. Crow ◽  
C. A. Bloxham ◽  
R. P. Parry ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Neurological Disease ◽  
Fibroblast Cell ◽  
Psychiatric Disease ◽  
Intracerebral Injection ◽  
New World Primates ◽  
Huntington's Chorea ◽  
Cytopathic Effects ◽  
Schizophrenic Patients

SynopsisIn experiments designed to investigate transmission, cerebrospinal fluid (CSF) from patients with schizophrenia and neurological disease (Huntington's chorea and multiple sclerosis) which had been found to induce cytopathic effects in human embryonic fibroblast cell culture was injected intracerebrally into mice, hamsters and marmosets (small New World primates). No evidence was obtained of transmission to mice or hamsters. A total of 15 marmosets (Callithrix jacchus) was injected intracerebrally with CSF [8 with samples from 4 patients with schizophrenia. 3 with samples from patients with neurological disease (2 with Huntington's chorea and 1 with multiple sclerosis) and 4 with samples from 3 patients without neurological or psychiatric disease] and was observed over a period of 2½ years.Analysis of variance on data obtained from behavioural observations averaged over 6-month periods revealed that animals injected with CSF from patients with schizophrenia and neurological disease became progressively more inactive when compared with animals injected with CSF from control patients. The change detected by behavioural observation was confirmed as a difference 2 and 2½ years after injection by automated activity monitoring.There was an incidence of reproductive anomalies (including two occipital encephalocoeles) in the females in the experimental group, but the numbers are too small to draw firm conclusions from this observation.Many reported differences in biological samples from schizophrenic patients and normal controls have subsequently been found to be due to factors unrelated to the disease state. This may prove to be the case with the changes observed in this experiment. Nevertheless, the fact that marmosets injected with CSF from patients suffering from neuropsychiatric disease, including schizophrenia, subsequently differed in their behaviour from those injected with control CSF warrants further investigation.

Rapid Fibroblast Cell Culture Characterization with Impedimetric Label‐free Heat Shock Protein – 47 Biosensor

2020 ◽  
Vol 32 (10) ◽  
pp. 2310-2315
Author(s):  
Duygu Harmanci ◽  
Zihni Onur Uygun ◽  
Ayşe Koçak Sezgin ◽  
Cenk Demirdöver ◽  
Ferhan Girgin Sagin ◽  
...  
Keyword(s):  
Cell Culture ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Fibroblast Cell ◽  
Label Free ◽  
Heat Shock Protein 47 ◽  
Fibroblast Cell Culture

Human foreskin fibroblasts: from waste bag to important biomedical applications

2018 ◽  
Vol 11 (6) ◽  
pp. 385-394 ◽  
Author(s):  
Thomaz Oliveira ◽  
Ilana Costa ◽  
Victor Marinho ◽  
Valécia Carvalho ◽  
Karla Uchôa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Experimental Studies ◽  
Embryonic Stem ◽  
Fibroblast Cell ◽  
Human Foreskin Fibroblast ◽  
Cellular Interactions ◽  
Human Foreskin Fibroblasts ◽  
Fibroblast Cell Culture ◽  
Human Foreskin Fibroblast Cell

Circumcision is one of the most performed surgical procedures worldwide, and it is estimated that one in three men worldwide is circumcised, which makes the preputial skin removed after surgery an abundant material for possible applications. In particular, it is possible efficiently to isolate the cells of the foreskin, with fibroblasts being the most abundant cells of the dermis and the most used in biomedical research. This work aimed to review the knowledge and obtain a broad view of the main applications of human foreskin fibroblast cell culture. A literature search was conducted, including clinical trials, preclinical basic research studies, reviews and experimental studies. Several medical and laboratory applications of human foreskin fibroblast cell culture have been described, especially when it comes to the use of human foreskin fibroblasts as feeder cells for the cultivation of human embryonic stem cells, in addition to co-culture with other cell types. The culture of foreskin fibroblasts has also been used to: obtain induced pluripotent stem cells; the diagnosis of Clostridium difficile; to test the toxicity and effect of substances on normal cells, especially the toxicity of possible antineoplastic drugs; in viral culture, mainly of the human cytomegalovirus, study of the pathogenesis of other microorganisms; varied studies of cellular physiology and cellular interactions. Fibroblasts are important for cell models for varied application cultures, demonstrating how the preputial material can be reused, making possible new applications. Level of evidence: Not applicable for this multicentre audit.

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