An Immunoelectron Microscopic Study of Type C Virus Particles in Feline Sarcoma Virus-Induced Rat Tumors

Many type C RNA tumor viruses can infect cells of different species. Following cross-species infection, these viruses are known to exhibit some altered properties. In an attempt to investigate host influence on properties of mammalian RNA tumor viruses, neonatal Wistar rats were inoculated with feline sarcoma virus of Snyder-Theilen strain. A transplantable tumor line designated as RT-FeSV was established from one of these induced tumors. Some syngeneic rats inoculated with RT-FeSV tumors which were passaged in rats less than 3 times developed precipitable serum antibodies to feline leukemia virus (FeLV).

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A Report Of Morphologically Aberrant Virus Particles in Cells Infected With MSV (FelLV) Virus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100073362 ◽

1973 ◽

Vol 31 ◽

pp. 584-585

Author(s):

R. A. Al-Adhami ◽

A. L. Chapman

Keyword(s):

Leukemia Virus ◽

Feline Leukemia Virus ◽

Virus Particles ◽

Induced Tumors ◽

Embryo Cells ◽

Murine Sarcoma Virus ◽

Sarcoma Virus ◽

Mouse Cells ◽

Type C ◽

Murine Sarcoma

Fujinaga et al reported MSV induced rat and hamster osteosarcoma which showed an occassional unusual bud in the rat induced tumors. Savage and Hackett and Hackett and Sylvester reported abnormal type C virus in UCLB cells derived from Balb/3t3 cells infected and transformed with MLV. They wer unable to demonstrate sarcoma virus activity. Fischinger and O‘Connor reported the infection of cat embryo cells by a centrifugally induced aggregate of murine sarcoma virus and feline leukemia virus designated as MSV(FelLV). This virus gave rise to a defective, focus forming virus which propagated in cat cells but not in mouse cells.In the present study the morphoiogy of the MSV(FelLV) virus obtained from Dr. Fischinger and maintained in our laboratory since 1970 will be reported. Feline embryo fibroblasts (established in our lab.) and Crandall feline kidney cells (Cutter-Haver-Lockhart, Shawnee, Kansas) were used in this study.

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Biological and Surface Properties of C-Type Virus Particles Produced by Novikoff Ascites Hepatoma Cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079395 ◽

1977 ◽

Vol 35 ◽

pp. 388-389

Author(s):

D.C. Hixson ◽

J.C. Chan ◽

J.M. Bowen ◽

E.F. Walborg

Keyword(s):

Surface Properties ◽

Ricinus Communis ◽

Rat Kidney ◽

Leukemia Virus ◽

Viral Envelope ◽

Virus Particles ◽

Chemically Induced ◽

Sarcoma Virus ◽

Hepatocarcinoma Cells ◽

Type C

Several years ago Karasaki (1) reported the production of type C virus particles by Novikoff ascites hepatocarcinoma cells. More recently, Weinstein (2) has reported the presence of type C virus particles in cell cultures derived from transplantable and primary hepatocellular carcinomas. To date, the biological function of these virus and their significance in chemically induced hepatocarcinogenesis are unknown. The present studies were initiated to determine a possible role for type C virus particles in chemically induced hepatocarcinogenesis. This communication describes results of studies on the biological and surface properties of type C virus associated with Novikoff hepatocarcinoma cells.Ecotropic and xenotropic murine leukemia virus (MuLV) activity in ascitic fluid of Novikoff tumor-bearing rats was assayed in murine sarcoma virus transformed S+L- mouse cells and S+L- mink cells, respectively. The presence of sarcoma virus activity was assayed in non-virus-producing normal rat kidney (NRK) cells. Ferritin conjugates of concanavalin A (Fer-Con wheat germ agglutinin (Fer-WGA), and Ricinus communis agglutinins I and II (Fer-RCAI and Fer-RCAII) were used to probe the structure and topography of saccharide determinants present on the viral envelope.

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Histopathologic and Ultrastructural Study of Tumors Induced by Murine Sarcoma Virus (MSV).

Tumori Journal ◽

10.1177/030089167506100202 ◽

1975 ◽

Vol 61 (2) ◽

pp. 129-150 ◽

Cited By ~ 1

Author(s):

Natale Pennelli ◽

Luigi Chieco-Bianchi ◽

Dino Collavo ◽

Attilio Cecchetto

Keyword(s):

Muscle Fibers ◽

Cell Types ◽

Neoplastic Tissue ◽

Virus Particles ◽

Induced Tumors ◽

Granulocyte Infiltration ◽

Murine Sarcoma Virus ◽

Sarcoma Virus ◽

Type C ◽

Murine Sarcoma

Various growth phases of Moloney murine sarcoma virus (M-MSV) induced tumors in suckling and young adult BALB/c mice have been studied by light and electron microscopy. In the early phase (3-6 days following M-MSV), observations at the injection site of the thigh muscles consisted of endo- and perimysial edema, «activated» muscle satellite cells, endothelial cells and fibroblasts, scattered type C virus particles within the muscle fibers, muscle fibers and endomysial cells undergoing necrosis and macrophage and granulocyte infiltration. During the overt tumor phase (6-12 days following M-MSV), observation of neoplastic tissue disclosed proliferation of several cell types (endothelial, periosteal, fibroblasts, etc.), poorly differentiated myoblasts along with atypical rhabdo-myoblast-like cells and sarcolytes, type C virus budding from muscle fiber and myoblast plasma membrane, and intense degenerative and regenerative changes in the muscle fibers together with more profuse granulocyte infiltration. The regressive phase (13-21 days following M-MSV) presented reduced cellularity of the neoplastic tissue, a decrease in blast cells, diminishing granulocyte infiltration with contemporaneous appearance of prominent lymphocyte foci and gradual disappearance of virus particles. Although many cell types of mesenchymal origin proliferate following M-MSV infection, the above morphological findings indicate that striated muscle is a preferential site for virus replication and transformation. Furthermore, the peculiar virus cell relationships leading to cell lysis and continuous recruitment of newly infected cells have been widely documented. In the light of these findings it is suggested that, besides the host immune control of virus spread and tumor cell multiplication, the non clonal growth pattern of M-MSV induced tumors is a crucial factor in determining the spontaneous regression which occurs with high frequency in this experimental system.

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Localization of gp70 and p30 Murine Type C. Virus Antigens in Thin Section Electron Microscopy Using Novel Immunolatex Spheres and Comparison with Immunoferritin and Immunoperoxidase Methods

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079358 ◽

1977 ◽

Vol 35 ◽

pp. 380-381

Author(s):

M.A. Gonda ◽

H. Hager ◽

S. Oroszlan ◽

R.V. Gilden ◽

K.C. Hsu

Keyword(s):

Thin Section ◽

Core Protein ◽

Leukemia Virus ◽

Structural Proteins ◽

Tumor Viruses ◽

Latex Spheres ◽

Section Electron ◽

Rauscher Leukemia ◽

Type C ◽

Rna Tumor Viruses

Mammalian type C RNA tumor viruses are composed of several structural proteins that can be identified by molecular weight and location on the virion (1). Rauscher leukemia virus (RLV), a murine type C virus, has a 70,000 dalton glycoprotein (gp70) in its envelope and a 30,000 dalton core protein (p30) as two of its major structural proteins (1,2). Synthetic latex microspheres have been shown to be useful surface markers for the scanning electron microscope (SEM) (3). Presented here is a thin section method utilizing novel latex spheres containing aromatic amines which, when coupled to antibodies by diazotization, act as electron-dense markers for indirect immunoelectron microscopic (IEM) detection of gp70 and p30 antigens of RLV. These results are compared with those obtained by immunoferritin and immunoperoxidase methods.RLV-infected murine JLSV-10 cells were used for all IEM. All reactions prior to fixation were carried out at 4°C. Pelleted cells were resuspended in primary serum, either goat anti-RLV p30, goat anti-RLV gp70, or normal goat, and allowed to react for 30 min. Cells were washed and sedimented 3X in buffer. Cell pellets for indirect immunolatex labeling were resuspended and gently agitated for 30 min in donkey anti-goat IgG coupled to latex spheres.

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Recent Studies on a Murine Leukemia Virus Grown in Tissue Culture

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060544 ◽

1967 ◽

Vol 25 ◽

pp. 106-107

Author(s):

L. Z. de Tkaczevski ◽

E. de Harven ◽

C. Friend

Keyword(s):

Tissue Culture ◽

Solid Tumors ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Supernatant Fluid ◽

Virus Particles ◽

Friend Leukemia ◽

Type C ◽

Leukemia Viruses ◽

Murine Leukemia

Despite extensive studies, the correlation between the morphology and pathogenicity of murine leukemia viruses (MLV) has not yet been clarified. The virus particles found in the plasma of leukemic mice belong to 2 distinct groups, 1 or 2% of them being enveloped A particles and the vast majority being of type C. It is generally believed that these 2 types of particles represent different phases in the development of the same virus. Particles of type A have been thought to be an earlier form of type C particles. One of the tissue culture lines established from Friend leukemia solid tumors has provided the material for the present study. The supernatant fluid of the line designated C-1A contains an almost pure population of A particles as illustrated in Figure 1. The ratio is, therefore, the reverse of what is unvariably observed in the plasma of leukemic mice where C particles predominate.

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