Biological and Surface Properties of C-Type Virus Particles Produced by Novikoff Ascites Hepatoma Cells

1977 ◽  
Vol 35 ◽  
pp. 388-389
Author(s):  
D.C. Hixson ◽  
J.C. Chan ◽  
J.M. Bowen ◽  
E.F. Walborg
Keyword(s):  
Surface Properties ◽  
Ricinus Communis ◽  
Rat Kidney ◽  
Leukemia Virus ◽  
Viral Envelope ◽  
Virus Particles ◽  
Chemically Induced ◽  
Sarcoma Virus ◽  
Hepatocarcinoma Cells ◽  
Type C

Several years ago Karasaki (1) reported the production of type C virus particles by Novikoff ascites hepatocarcinoma cells. More recently, Weinstein (2) has reported the presence of type C virus particles in cell cultures derived from transplantable and primary hepatocellular carcinomas. To date, the biological function of these virus and their significance in chemically induced hepatocarcinogenesis are unknown. The present studies were initiated to determine a possible role for type C virus particles in chemically induced hepatocarcinogenesis. This communication describes results of studies on the biological and surface properties of type C virus associated with Novikoff hepatocarcinoma cells.Ecotropic and xenotropic murine leukemia virus (MuLV) activity in ascitic fluid of Novikoff tumor-bearing rats was assayed in murine sarcoma virus transformed S+L- mouse cells and S+L- mink cells, respectively. The presence of sarcoma virus activity was assayed in non-virus-producing normal rat kidney (NRK) cells. Ferritin conjugates of concanavalin A (Fer-Con wheat germ agglutinin (Fer-WGA), and Ricinus communis agglutinins I and II (Fer-RCAI and Fer-RCAII) were used to probe the structure and topography of saccharide determinants present on the viral envelope.

An Immunoelectron Microscopic Study of Type C Virus Particles in Feline Sarcoma Virus-Induced Rat Tumors

1974 ◽  
Vol 32 ◽  
pp. 246-247
Author(s):  
K. Maruyama ◽  
M. F. Miller ◽  
S. H. Wagner ◽  
L. Dmochowski
Keyword(s):  
Leukemia Virus ◽  
Feline Leukemia Virus ◽  
Virus Particles ◽  
Tumor Viruses ◽  
Induced Tumors ◽  
Sarcoma Virus ◽  
Type C ◽  
Rat Tumors ◽  
Rna Tumor Viruses ◽  
Host Influence

Many type C RNA tumor viruses can infect cells of different species. Following cross-species infection, these viruses are known to exhibit some altered properties. In an attempt to investigate host influence on properties of mammalian RNA tumor viruses, neonatal Wistar rats were inoculated with feline sarcoma virus of Snyder-Theilen strain. A transplantable tumor line designated as RT-FeSV was established from one of these induced tumors. Some syngeneic rats inoculated with RT-FeSV tumors which were passaged in rats less than 3 times developed precipitable serum antibodies to feline leukemia virus (FeLV).

A Report Of Morphologically Aberrant Virus Particles in Cells Infected With MSV (FelLV) Virus

1973 ◽  
Vol 31 ◽  
pp. 584-585
Author(s):  
R. A. Al-Adhami ◽  
A. L. Chapman
Keyword(s):  
Leukemia Virus ◽  
Feline Leukemia Virus ◽  
Virus Particles ◽  
Induced Tumors ◽  
Embryo Cells ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Mouse Cells ◽  
Type C ◽  
Murine Sarcoma

Fujinaga et al reported MSV induced rat and hamster osteosarcoma which showed an occassional unusual bud in the rat induced tumors. Savage and Hackett and Hackett and Sylvester reported abnormal type C virus in UCLB cells derived from Balb/3t3 cells infected and transformed with MLV. They wer unable to demonstrate sarcoma virus activity. Fischinger and O‘Connor reported the infection of cat embryo cells by a centrifugally induced aggregate of murine sarcoma virus and feline leukemia virus designated as MSV(FelLV). This virus gave rise to a defective, focus forming virus which propagated in cat cells but not in mouse cells.In the present study the morphoiogy of the MSV(FelLV) virus obtained from Dr. Fischinger and maintained in our laboratory since 1970 will be reported. Feline embryo fibroblasts (established in our lab.) and Crandall feline kidney cells (Cutter-Haver-Lockhart, Shawnee, Kansas) were used in this study.

Recent Studies on a Murine Leukemia Virus Grown in Tissue Culture

1967 ◽  
Vol 25 ◽  
pp. 106-107
Author(s):  
L. Z. de Tkaczevski ◽  
E. de Harven ◽  
C. Friend
Keyword(s):  
Tissue Culture ◽  
Solid Tumors ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Supernatant Fluid ◽  
Virus Particles ◽  
Friend Leukemia ◽  
Type C ◽  
Leukemia Viruses ◽  
Murine Leukemia

Despite extensive studies, the correlation between the morphology and pathogenicity of murine leukemia viruses (MLV) has not yet been clarified. The virus particles found in the plasma of leukemic mice belong to 2 distinct groups, 1 or 2% of them being enveloped A particles and the vast majority being of type C. It is generally believed that these 2 types of particles represent different phases in the development of the same virus. Particles of type A have been thought to be an earlier form of type C particles. One of the tissue culture lines established from Friend leukemia solid tumors has provided the material for the present study. The supernatant fluid of the line designated C-1A contains an almost pure population of A particles as illustrated in Figure 1. The ratio is, therefore, the reverse of what is unvariably observed in the plasma of leukemic mice where C particles predominate.

Histopathologic and Ultrastructural Study of Tumors Induced by Murine Sarcoma Virus (MSV).

1975 ◽  
Vol 61 (2) ◽  
pp. 129-150 ◽  
Author(s):  
Natale Pennelli ◽  
Luigi Chieco-Bianchi ◽  
Dino Collavo ◽  
Attilio Cecchetto
Keyword(s):  
Muscle Fibers ◽  
Cell Types ◽  
Neoplastic Tissue ◽  
Virus Particles ◽  
Induced Tumors ◽  
Granulocyte Infiltration ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Type C ◽  
Murine Sarcoma

Various growth phases of Moloney murine sarcoma virus (M-MSV) induced tumors in suckling and young adult BALB/c mice have been studied by light and electron microscopy. In the early phase (3-6 days following M-MSV), observations at the injection site of the thigh muscles consisted of endo- and perimysial edema, «activated» muscle satellite cells, endothelial cells and fibroblasts, scattered type C virus particles within the muscle fibers, muscle fibers and endomysial cells undergoing necrosis and macrophage and granulocyte infiltration. During the overt tumor phase (6-12 days following M-MSV), observation of neoplastic tissue disclosed proliferation of several cell types (endothelial, periosteal, fibroblasts, etc.), poorly differentiated myoblasts along with atypical rhabdo-myoblast-like cells and sarcolytes, type C virus budding from muscle fiber and myoblast plasma membrane, and intense degenerative and regenerative changes in the muscle fibers together with more profuse granulocyte infiltration. The regressive phase (13-21 days following M-MSV) presented reduced cellularity of the neoplastic tissue, a decrease in blast cells, diminishing granulocyte infiltration with contemporaneous appearance of prominent lymphocyte foci and gradual disappearance of virus particles. Although many cell types of mesenchymal origin proliferate following M-MSV infection, the above morphological findings indicate that striated muscle is a preferential site for virus replication and transformation. Furthermore, the peculiar virus cell relationships leading to cell lysis and continuous recruitment of newly infected cells have been widely documented. In the light of these findings it is suggested that, besides the host immune control of virus spread and tumor cell multiplication, the non clonal growth pattern of M-MSV induced tumors is a crucial factor in determining the spontaneous regression which occurs with high frequency in this experimental system.

Restricted expression of integrated primate type-C virus (Gibbon ape leukemia virus-simian sarcoma virus) proviral DNA in nonproductively infected human B lymphoblasts

Virology ◽  
1982 ◽  
Vol 117 (1) ◽  
pp. 195-206 ◽  
Author(s):  
L. Ceccherini Nelli ◽  
R. Dalla-Favera ◽  
P.D. Markham ◽  
F.W. Ruscetti ◽  
F. Wong-Staal ◽  
...  
Keyword(s):  
Leukemia Virus ◽  
Proviral Dna ◽  
Sarcoma Virus ◽  
Simian Sarcoma Virus ◽  
Type C ◽  
Gibbon Ape Leukemia Virus

scholarly journals MUTANTS OF NONPRODUCER CELL LINES TRANSFORMED BY MURINE SARCOMA VIRUSES

1973 ◽  
Vol 138 (2) ◽  
pp. 356-363 ◽  
Author(s):  
M. Hatanaka ◽  
R. Klein ◽  
R. Toni ◽  
J. Walker ◽  
R. Gilden
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Virus Particles ◽  
Tumorigenic Potential ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Type C ◽  
Murine Sarcoma ◽  
Tumorigenic Cell

A variety of cell mutants were obtained by a single 5'-bromodeoxyuridine (BrdU) treatment of an nonproducer (NP) cell line transformed by the Kirsten strain of murine sarcoma virus (Ki-MSV). Isolation procedures of these cell See PDF for Structure mutants are described. The cell mutants obtained were classified by tumorigenic potential and shedding of Type C virus particles. The cell mutants were classified into four groups: (A) tumorigenic, without particles; (B) tumorigenic, with Type C particles; (C) nontumorigenic, without particles; and (D) nontumorigenic, with Type C particles. The tumorigenic cell lines showed variability in morphology with both flat and typical transformed appearing cell lines showing equal transplantability.

Studies on Spontaneous Mammary Tumors in High-Leukemia-Strain Mice and on Mammary Tumors Induced by Mouse Leukemia Virus in Low-Leukemia-Strain Mice

1967 ◽  
Vol 25 ◽  
pp. 108-109
Author(s):  
T. Yumoto ◽  
L. Young ◽  
W. C. Williams ◽  
L. Dmochowski
Keyword(s):  
Mammary Gland ◽  
Mammary Tumor ◽  
Leukemia Virus ◽  
Medullary Carcinoma ◽  
Mammary Tumors ◽  
Tumor Type ◽  
Virus Particles ◽  
Strain Mouse ◽  
Type C ◽  
Murine Leukemia

Murine leukemia type C virus particles were found in a spontaneous mammary gland medullary carcinoma of a 9-month-old high-leukemia AKR strain mouse (Fig.1). Similar virus particles were observed in a spontaneous mammary gland adenocarcinoma of a 12-month-old SJL/J high-leukemia-strain mouse (Fig.2). No virus particles could be seen in another mammary tumor of similar type in a 13-month-old SJL/J strain mouse nor in a spontaneous mammary squamous cell carcinoma of a 9-month-old NZB/BL high-leukemia-strain mouse.Mammary tumor type B virus particles were observed in a mammary gland adenocarcinoma of a 6-month-old AKR strain mouse inoculated when newborn with Moloney leukemia virus (Fig.3).No virus particles could be found in a mammary gland medullary carcinoma of a 12-month-old BALB/c low-leukemia and low-mammary-cancer-strain mouse inoculated with Moloney virus.Mammary gland medullary carcinomas developed in two BALB/c strain 5-and 6-month old mice inoculated with Rauscher leukemia virus. Murine leukemia type C virus particles were observed in both tumors (Fig.4).

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