Application of the Short Focal Length Objective to Biological Substances

1967 ◽  
Vol 25 ◽  
pp. 224-225
Author(s):  
W. W Harri
Keyword(s):  
Molecular Structure ◽  
Electron Microscope ◽  
Serum Albumin ◽  
Plasma Protein ◽  
Transmission Electron Microscope ◽  
Focal Length ◽  
Resolving Power ◽  
Transmission Electron ◽  
Short Focal Length ◽  
Conventional Transmission Electron Microscope

Possible improvement in resolving power by use of objective focal lengths of 2 mm in the conventional transmission electron microscope has been described by Heidenreich and Armbruster and by H. Fernandez-Moran. A modified Siemens Elmiskop IA has been used to examine a plasma protein, some polyamino acids, sRNA, 50s ribosome preparations, serum albumin, and myoglobin in unsupported specimens.Characteristics of the images obtained in defocussed images will be discussed in relation to the molecular structure of these compounds.

A Simple Method of the Electric/Magnetic Field Observation by a Conventional Transmission Electron Microscope

2005 ◽  
Vol 475-479 ◽  
pp. 4029-4034 ◽  
Author(s):  
Katsuhiro Sasaki ◽  
Hiroyasu Saka
Keyword(s):  
Magnetic Field ◽  
Electron Microscope ◽  
Field Distribution ◽  
Transmission Electron Microscope ◽  
Electrostatic Field ◽  
Field Observation ◽  
Simple Method ◽  
Transmission Electron ◽  
Novel Method ◽  
Conventional Transmission Electron Microscope

A novel method to observe the electrostatic field distribution with a conventional transmission electron microscope has been developed. The method allows measurements of a potential difference less than 1V/µm. This method can be performed in any kind of conventional transmission electron microscope and applied to the observation of the electric/magnetic field at the level of a specimen.

Improving a conventional transmission electron microscope for high resolution

1979 ◽  
Vol 4 (4) ◽  
pp. 473-477 ◽  
Author(s):  
Tetsuo Oikawa ◽  
Chikara Kimura ◽  
Kiichi Hojou ◽  
Norio Baba ◽  
Koichi Kanaya
Keyword(s):  
Electron Microscope ◽  
High Resolution ◽  
Transmission Electron Microscope ◽  
Transmission Electron ◽  
Conventional Transmission Electron Microscope

scholarly journals Substructure of Wool Cortical Cells

1971 ◽  
Vol 24 (4) ◽  
pp. 1355 ◽  
Author(s):  
CA Anderson ◽  
JD Leeder
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Resolving Power ◽  
Specimen Preparation ◽  
Cortical Cells ◽  
Transmission Electron ◽  
Very High

The morphology of wool fibres and their components has been studied mainly with the optical and transmission electron microscope. In the former, the specimen is observed directly but the resolution is poor; in the latter, the resolving power is very high but the introduction of artefacts during specimen preparation and the indirect nature of the data obtained lead to possible uncertainties in interpretation.

Characterization of beam-induced thinning and shrinkage of semi-thick sections in the HVEM

1990 ◽  
Vol 48 (3) ◽  
pp. 752-753
Author(s):  
J.R. Kremer ◽  
E.T. O'Toole ◽  
G.P. Wray ◽  
D.M. Mastronarde ◽  
S.J. Mitchell ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Colloidal Gold ◽  
Similar Data ◽  
Thin Sections ◽  
Gold Particles ◽  
Dose Rates ◽  
Transmission Electron ◽  
Conventional Transmission Electron Microscope

It is well known that irradiation of plastic sections in a conventional transmission electron microscope (cTEM) causes specimen thinning and distortion. Thinning has been observed in the cTEM using several embedding media, using methods such as shrinkage of ordered paracrystalline structures, and shrinkage of sections coated with colloidal gold markers. The total thinning observed in the cTEM (80kev) is 30-50% for thin sections of epon araldite, but similar data do not exist for the HVEM at 1000 kev. Here we describe beam induced thinning and shrinkage of 0.2um sections in the HVEM.Experiments were performed using 0.2um sections of EPOX 812/Araldite or LX112 with 15 nm and 30 nm gold particles affixed to either surface of the section. The sections were initially tilted to approximately 25° and irradiated with known dose rates. Micrographs were taken at different times between 0-20 minutes then the sections were tilted back to 0° for a reference micrograph.

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