Replication of type C Virus Particles in Burkitt Lymphoma Cells in Vitro Treated with Feline or Murine Leukemia Virus

In the course of studies on possible interaction between animal leukemia viruses and a hypothetical human cancer virus(es), a culture line (P3) derived from Burkitt lymphoma and containing herpestype virus was inoculated 10 times with feline or murine leukemia virus. Filtrates (0.45 µ Millipore) of tissue culture fluid from feline lymphoma cell cultures containing type C particles (supplied by Dr. C. G. Rickard) and from human embryo kidney cell cultures infected with Rauscher leukemia virus(supplied by Dr. B. S. Wright) were used as source of virus. Electron micro scopy was carried out on cells from cultures before inoculation, the 4-day-old culture after 10 inoculations, and subcultures at 2nd (30 days), 3rd (40 days), 12th(85 or 95 days) passages after the last inoculation.

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The endogenous mink cell focus-forming (MCF) gp70 linked to the Rmcf gene restricts MCF virus replication in vivo and provides partial resistance to erythroleukemia induced by Friend murine leukemia virus.

Journal of Experimental Medicine ◽

10.1084/jem.167.5.1535 ◽

1988 ◽

Vol 167 (5) ◽

pp. 1535-1546 ◽

Cited By ~ 14

Author(s):

R S Buller ◽

M Sitbon ◽

J L Portis

Keyword(s):

Cell Cultures ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Backcross Progeny ◽

Genetic Studies ◽

Mink Cell ◽

Current Report ◽

Murine Leukemia

The Rmcf locus restricts the in vitro replication of mink cell focus-forming (MCF) viruses in cell cultures derived from mice carrying the resistance allele. Previously we reported that in cell cultures from first backcross progeny, this Rmcf-linked restriction segregates with the expression of an endogenous retroviral gp70 serologically related to that of MCF viruses. The current report details the results of genetic studies designed to examine the possible association of this endogenous gp70 with resistance of mice to Friend murine leukemia virus (F-MuLV)-induced erythroleukemia. This env gene segregates as a single dominant trait in (DBA/2 X IRW) X IRW progeny, in which the expression of the gene can be detected by serological techniques. Results indicated that the gp70- progeny developed leukemia at the same rate as the susceptible IRW parent, whereas the tempo of disease among the gp70+ progeny was significantly slower. However, the resistance mediated by this gene was only partial, since most of the gp70+ offspring eventually developed erythroleukemia when followed for 6 mo. This endogenous gp70 also segregated with a restriction to the expression of recombinant MCF viruses after infection with F-MuLV. Since in this study all unlinked genes segregated independently, this is direct evidence that MCF viruses participate in the induction of erythroleukemia.

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Recent Studies on a Murine Leukemia Virus Grown in Tissue Culture

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060544 ◽

1967 ◽

Vol 25 ◽

pp. 106-107

Author(s):

L. Z. de Tkaczevski ◽

E. de Harven ◽

C. Friend

Keyword(s):

Tissue Culture ◽

Solid Tumors ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Supernatant Fluid ◽

Virus Particles ◽

Friend Leukemia ◽

Type C ◽

Leukemia Viruses ◽

Murine Leukemia

Despite extensive studies, the correlation between the morphology and pathogenicity of murine leukemia viruses (MLV) has not yet been clarified. The virus particles found in the plasma of leukemic mice belong to 2 distinct groups, 1 or 2% of them being enveloped A particles and the vast majority being of type C. It is generally believed that these 2 types of particles represent different phases in the development of the same virus. Particles of type A have been thought to be an earlier form of type C particles. One of the tissue culture lines established from Friend leukemia solid tumors has provided the material for the present study. The supernatant fluid of the line designated C-1A contains an almost pure population of A particles as illustrated in Figure 1. The ratio is, therefore, the reverse of what is unvariably observed in the plasma of leukemic mice where C particles predominate.

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Sequential activation of the three protomers in the Moloney murine leukemia virus Env

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1617264114 ◽

2017 ◽

Vol 114 (10) ◽

pp. 2723-2728 ◽

Cited By ~ 3

Author(s):

Mathilda Sjöberg ◽

Robin Löving ◽

Birgitta Lindqvist ◽

Henrik Garoff

Keyword(s):

Murine Leukemia Virus ◽

Leukemia Virus ◽

Moloney Murine Leukemia Virus ◽

Virus Envelope ◽

Sequential Activation ◽

Virus Envelope Protein ◽

Membrane Fusion Proteins ◽

Viral Membrane Fusion ◽

Murine Leukemia

Viral membrane fusion proteins of class I are trimers in which the protomeric unit is a complex of a surface subunit (SU) and a fusion active transmembrane subunit (TM). Here we have studied how the protomeric units of Moloney murine leukemia virus envelope protein (Env) are activated in relation to each other, sequentially or simultaneously. We followed the isomerization of the SU-TM disulfide and subsequent SU release from Env with biochemical methods and found that this early activation step occurred sequentially in the three protomers, generating two asymmetric oligomer intermediates according to the scheme (SU-TM)3→ (SU-TM)2TM → (SU-TM)TM2→ TM3. This was the case both when activation was triggered in vitro by depleting stabilizing Ca2+from solubilized Env and when viral Env was receptor triggered on rat XC cells. In the latter case, the activation reaction was too fast for direct observation of the intermediates, but they could be caught by alkylation of the isomerization active thiol.

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Heterogeneity of Murine Leukemia Virus in vitro DNA; Detection of Viral DNA in Mammalian Cells

Science ◽

10.1126/science.172.3990.1353 ◽

1971 ◽

Vol 172 (3990) ◽

pp. 1353-1355 ◽

Cited By ~ 55

Author(s):

L. D. Gelb ◽

S. A. Aaronson ◽

M. A. Martin

Keyword(s):

Murine Leukemia Virus ◽

Mammalian Cells ◽

Dna Detection ◽

Leukemia Virus ◽

Viral Dna ◽

Murine Leukemia

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Novel GACG-hairpin pair motif in the 5' untranslated region of type C retroviruses related to murine leukemia virus.

Journal of Virology ◽

10.1128/jvi.66.2.632-640.1992 ◽

1992 ◽

Vol 66 (2) ◽

pp. 632-640 ◽

Cited By ~ 40

Author(s):

D A Konings ◽

M A Nash ◽

J V Maizel ◽

R B Arlinghaus

Keyword(s):

Murine Leukemia Virus ◽

Untranslated Region ◽

Leukemia Virus ◽

Type C ◽

Murine Leukemia

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Mus cervicolor Murine Leukemia Virus Isolate M813 Belongs to a Unique Receptor Interference Group

Journal of Virology ◽

10.1128/jvi.75.10.4490-4498.2001 ◽

2001 ◽

Vol 75 (10) ◽

pp. 4490-4498 ◽

Cited By ~ 12

Author(s):

Vladimir Prassolov ◽

Sibyll Hein ◽

Marion Ziegler ◽

Dmitry Ivanov ◽

Carsten Münk ◽

...

Keyword(s):

Host Range ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Cell Entry ◽

Chromosome 2 ◽

Coding Region ◽

Common Receptor ◽

Type C ◽

Murine Leukemia ◽

Receptor Interference

ABSTRACT Murine leukemia virus (MuLV) M813 was originally isolated from the Southeast Asian rodent Mus cervicolor. As with the ecotropic MuLVs derived from Mus musculus, its host range is limited to rodent cells. Earlier studies have mapped its receptor to chromosome 2, but it has not been established whether M813 shares a common receptor with any other MuLVs. In this study, we have performed interference assays with M813 and viruses from four interference groups of MuLV. The infection efficiency of M813 was not compromised in cells expressing any one of the other MuLVs, demonstrating that M813 must use a distinct receptor for cell entry. The entire M813 env coding region was molecularly cloned. Sequence analysis revealed high similarity with other MuLVs but with a unique receptor-binding domain. Substitution of M813env sequences in Moloney MuLV resulted in a replication-competent virus with a host range and interference profile similar to those of the biological clone M813. M813 thus defines a novel receptor interference group of type C MuLVs.

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Strong selection for cells containing new ecotropic recombinant murine leukemia virus provirus after propagation of C57BL/6 radiation-induced thymoma cells in vitro or in vivo

Molecular and Cellular Biology ◽

10.1128/mcb.3.9.1675-1679.1983 ◽

1983 ◽

Vol 3 (9) ◽

pp. 1675-1679

Author(s):

P Jolicoeur ◽

E Rassart ◽

P Sankar-Mistry

Keyword(s):

Cell Lines ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Primary Radiation ◽

Secondary Tumors ◽

Radiation Induced ◽

Viral Sequences ◽

Murine Leukemia

Using the Southern procedure, we have studied the presence of ecotropic-specific murine leukemia viral sequences in genomic DNA isolated from primary X-ray-induced thymomas, from lymphoid cell lines established from them, or from secondary tumors passaged in vivo. We found that primary radiation-induced thymomas and infiltrated spleens do not harbor newly acquired ecotropic provirus. However, additional ecotropic proviruses (which appear recombinant in the gagpol region) could be detected in most of the tumorigenic cell lines established in vitro from them and in tumors arising from subcutaneous transplantation of the primary thymomas. These results suggest that primary radiation-induced thymomas may not be clonal. They also indicate a strong correlation between the presence of ecotropic recombinant proviruses in the genome and the growth ability, both in vitro and in vivo, of specific cells within these thymomas, suggesting a possible mitogenic function for murine leukemia virus.

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