Ultrastructure of myoepithelial cell in parotid gland

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100103772 ◽

1988 ◽

Vol 46 ◽

pp. 342-343

Author(s):

C. N. Sun

Keyword(s):

Parotid Gland ◽

Osmium Tetroxide ◽

Individual Cell ◽

Branching Processes ◽

Muscle Cells ◽

Myoepithelial Cells ◽

Uranyl Acetate ◽

Thin Sections ◽

Gland Carcinoma ◽

Parotid Gland Carcinoma

Myoepithelial cells have been observed in the prostate, harderian, apocrine, exocrine sweat and mammary glands. Such cells and their numerous branching processes form basket-like structures around the glandular acini. Their shapes are quite different from structures seen either in spindleshaped smooth muscle cells or skeletal muscle cells. These myoepithelial cells lie on the epithelial side of the basement membrane in the glands. This presentation describes the ultrastructure of such myoepithelial cells which have been found also in the parotid gland carcinoma from a 45-year old patient.Specimens were cut into small pieces about 1 mm3 and immediately fixed in 4 percent glutaraldehyde in phosphate buffer for two hours, then post-fixed in 1 percent buffered osmium tetroxide for 1 hour. After dehydration, tissues were embedded in Epon 812. Thin sections were stained with uranyl acetate and lead citrate. Ultrastructurally, the pattern of each individual cell showed wide variations.

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A Rare De Novo Myoepithelial Carcinoma Ex Pleomorphic Adenoma in a Young Woman

Case Reports in Otolaryngology ◽

10.1155/2020/8325374 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6

Author(s):

Geeta Ahuja ◽

Delaram J. Taghipour ◽

Olubode A. Olufajo ◽

Bonnie C. Davis ◽

Babak Shokrani ◽

...

Keyword(s):

Parotid Gland ◽

Pleomorphic Adenoma ◽

De Novo ◽

Myoepithelial Cells ◽

Young Female ◽

Accurate Diagnosis ◽

Carcinoma Ex Pleomorphic Adenoma ◽

Gland Carcinoma ◽

Uncommon Neoplasm ◽

Parotid Gland Carcinoma

Carcinoma ex pleomorphic adenoma, an uncommon neoplasm of the parotid gland, accounts for less than 4% of salivary gland tumors. It arises from a benign pleomorphic adenoma presenting in the sixth to eighth decades of life. We present this as a unique account of a primary parotid gland carcinoma, arising from myoepithelial cells, without a known precursor lesion, in a 28-year-old woman. This presentation seeks to provide familiarity of an unusual presentation of an unexpected rare pathology in a young female patient and the tools utilized for an accurate diagnosis.

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Membranous alterations in the cytoplasm and nucleus in a primitive neuroectodermal tumor of the brain

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100084016 ◽

1978 ◽

Vol 36 (2) ◽

pp. 628-629

Author(s):

C. N. Sun ◽

C. Araoz ◽

H. J. White

Keyword(s):

Nuclear Envelope ◽

Tumor Cells ◽

Osmium Tetroxide ◽

Primitive Neuroectodermal Tumor ◽

Uranyl Acetate ◽

Neuroectodermal Tumor ◽

Annulate Lamellae ◽

Lead Citrate ◽

Thin Sections ◽

The Brain

The ultrastructure of a cerebral primitive neuroectodermal tumor has been reported previously. In the present case, we will present some unusual previously unreported membranous structures and alterations in the cytoplasm and nucleus of the tumor cells.Specimens were cut into small pieces about 1 mm3 and immediately fixed in 4% glutaraldehyde in phosphate buffer for two hours, then post-fixed in 1% buffered osmium tetroxide for one hour. After dehydration, tissues were embedded in Epon 812. Thin sections were stained with uranyl acetate and lead citrate.In the cytoplasm of the tumor cells, we found paired cisternae (Fig. 1) and annulate lamellae (Fig. 2) noting that the annulate lamellae were sometimes associated with the outer nuclear envelope (Fig. 3). These membranous structures have been reported in other tumor cells. In our case, mitochondrial to nuclear envelope fusions were often noted (Fig. 4). Although this phenomenon was reported in an oncocytoma, their frequency in the present study is quite striking.

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Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141366 ◽

1995 ◽

Vol 53 ◽

pp. 998-999

Author(s):

J.M. Minda ◽

E. Dessy ◽

G. G. Pietra

Keyword(s):

Smooth Muscle ◽

Smooth Muscle Cells ◽

Room Temperature ◽

Osmium Tetroxide ◽

Muscle Cells ◽

Ultrastructural Localization ◽

Reproductive Age ◽

Thin Sections ◽

Smooth Muscle Proliferation ◽

Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

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Observations of thick and thin sections in a field-emission SEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100172826 ◽

1994 ◽

Vol 52 ◽

pp. 1018-1019

Author(s):

W. P. Wergin ◽

S. Roy ◽

E. F. Erbe ◽

C. A. Murphy ◽

C. D. Pooley

Keyword(s):

Electron Microscope ◽

Field Emission ◽

Room Temperature ◽

Osmium Tetroxide ◽

Uranyl Acetate ◽

Chemical Fixation ◽

Thin Sections ◽

Transmission Electron ◽

Conventional Transmission Electron Microscope ◽

Scanning Electron

Larvae of the nematode, Steinernema carpocapsae Weiser strain All, were cryofixed and freezesubstituted for 3 days in acetone containing 2% osmium tetroxide according to established procedures. Following chemical fixation, the nematodes were brought to room temperature, embedded in Spurr's medium and sectioned for observation with a Hitachi S-4100 field emission scanning electron microscope that was equipped with an Oxford CT 1500 Cryotrans System. Thin sections, about 80 nm thick, similar to those generally used in conventional transmission electron microscope (TEM) studies were mounted on copper grids and stained with uranyl acetate for 30 min and lead citrate for 5 min. Sections about 2 μm thick were also mounted and stained in a similar fashion. The grids were mounted on an Oxford grid holder, inserted into the microscope and onto a cryostage that was operated at ambient temperature. Thick and thin sections of the larvae were evaluated and photographed in the SEM at different accelerating voltages. Figs. 4 and 5 have undergone contrast conversion so that the images would resemble transmitted electron micrographs obtained with a TEM.

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Pretherapeutic Predictive Factors for Histological High‐Grade Parotid Gland Carcinoma

The Laryngoscope ◽

10.1002/lary.29728 ◽

2021 ◽

Author(s):

Takuya Mikoshiba ◽

Hiroyuki Ozawa ◽

Yoshihiro Watanabe ◽

Miho Kawaida ◽

Mariko Sekimizu ◽

...

Keyword(s):

Parotid Gland ◽

Predictive Factors ◽

High Grade ◽

Gland Carcinoma ◽

Parotid Gland Carcinoma

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54 ORAL Prediction of recurrence in parotid gland carcinoma

European Journal of Surgical Oncology ◽

10.1016/s0748-7983(06)70489-9 ◽

2006 ◽

Vol 32 ◽

pp. S18

Author(s):

J.F. Carrillo ◽

R. Vazquez ◽

F.J. Ochoa-Carrillo ◽

L.F. Oñate-Ocaña ◽

R. Herrera-Goepfert R

Keyword(s):

Parotid Gland ◽

Gland Carcinoma ◽

Parotid Gland Carcinoma

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ULTRASTRUCTURAL INTERRELATIONSHIPS OF NERVE PROCESSES AND SMOOTH MUSCLE CELLS IN THREE DIMENSIONS

The Journal of Cell Biology ◽

10.1083/jcb.28.1.37 ◽

1966 ◽

Vol 28 (1) ◽

pp. 37-49 ◽

Cited By ~ 54

Author(s):

J. C. Thaemert

Keyword(s):

Smooth Muscle ◽

Smooth Muscle Cells ◽

Osmium Tetroxide ◽

Three Dimensional ◽

Muscle Cells ◽

Uranyl Acetate ◽

Intestinal Wall ◽

Three Dimensions ◽

Serial Sections ◽

Muscularis Externa

The muscularis externa of the intestinal wall of frogs was fixed in osmium tetroxide, embedded in Vestopal-W, serially sectioned for electron microscopy, and stained with uranyl acetate. A method to obtain individually mounted and properly positioned serial sections is described. The three-dimensional techniques used during the course of this investigation demonstrate that it is possible to examine carefully relatively large areas of tissue on individual serial sections with the electron microscope and subsequently to construct montages of electron micrographs of pertinent areas from each section. Several carefully rendered interrelationships of nerve processes and smooth muscle cells in three dimensions are exhibited and described. Recent studies of other neuro-effector relationships are discussed in relation to the present status of the nature and organization of the autonomic nervous system in visceral organs.

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