Correlative light and transmission electron microscopy of tubular aggregates in skeletal muscle

1992 ◽  
Vol 50 (1) ◽  
pp. 660-661
Author(s):  
Thomas G. Manfredi ◽  
Wenjing Ding ◽  
Roderick Bronson
Keyword(s):  
Electron Microscopy ◽  
Skeletal Muscle ◽  
Transmission Electron Microscopy ◽  
Animal Models ◽  
Muscle Pain ◽  
Periodic Paralysis ◽  
Human Muscle ◽  
Tubular Aggregates ◽  
Transmission Electron ◽  
Gender Specific

Tubular aggregates (TAs) have been identified with a number of myopathies in humans. Periodic paralysis and muscle pain are frequently associated with TAs. Very little is known about the functional and anatomical significance of TAs in myopathic and aging human muscle. Recently, animal models for TAs have been identified which suggested that TAs are gender specific. However, recent studies suggest a need for more controls.

scholarly journals Handling Cell Culture Monolayers for Transmission Electron Microscopy

2013 ◽  
Vol 21 (3) ◽  
pp. 36-39 ◽  
Author(s):  
Leona Cohen-Gould
Keyword(s):  
Electron Microscopy ◽  
Skeletal Muscle ◽  
Cell Culture ◽  
Transmission Electron Microscopy ◽  
Cells In Culture ◽  
Area Of Interest ◽  
Transmission Electron

In our Core Facility, we are presented with a wide variety of tissue and sample types to process for TEM observation. Some tissues, such as liver, do not present orientation problems. Other tissues such as skeletal muscle have specific orientations that must be maintained. As long as the initial dissection of the tissue is done so that the orientation or area of interest can be identified, these samples do not present a problem. Likewise, when it is not necessary to maintain orientation, cells in culture can be treated with trypsin, to release them from the plate, and pelleted. Or the monolayer can be fixed in vitro and then scraped and pelleted, and the pellet can be processed while adherent to the wall of the Eppendorf tube or handled like a small piece of tissue, among other methods.

Sarcocystis species in skeletal muscle of otter (Lutra lutra)

Parasitology ◽  
1999 ◽  
Vol 118 (1) ◽  
pp. 59-62 ◽  
Author(s):  
K. WAHLSTRÖM ◽  
T. NIKKILÄ ◽  
A. UGGLA
Keyword(s):  
Electron Microscopy ◽  
Skeletal Muscle ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Lutra Lutra ◽  
Sarcocystis Species ◽  
Transmission Electron ◽  
Large Numbers ◽  
In Captivity ◽  
Sarcocyst Wall

Cysts of a Sarcocystis species were found in large numbers in skeletal muscle of an otter (Lutra lutra) which was raised in Norway and died in captivity in Sweden. This is the first report of Sarcocystis infection in the otter. The sarcocysts were 0·3–2·3 mm long and 0·06–0·25 mm wide. As judged by light microscopy the sarcocyst walls were thin (<3 μm) with a serrated surface but without visible projections. By transmission electron microscopy, the sarcocyst wall measured 0·6–1·8 μm and had minute undulations covering the entire sarcocyst surface giving the wall a wavy appearance. Septa were indistinct. The sarcocysts contained few metrocytes and numerous bradyzoites. Sarcocysts were not found in 69 other otters subjected to necropsy in Sweden.

scholarly journals Autophagy is affected in patients with hypokalemic periodic paralysis: an involvement in vacuolar myopathy?

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Thomas O. Krag ◽  
Sonja Holm-Yildiz ◽  
Nanna Witting ◽  
John Vissing
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Gene Transcription ◽  
Systemic Effect ◽  
Periodic Paralysis ◽  
Muscle Fibres ◽  
Hypokalemic Periodic Paralysis ◽  
Vacuolar Myopathy ◽  
Autophagy Gene ◽  
Transmission Electron

AbstractHypokalemic periodic paralysis is an autosomal dominant, rare disorder caused by variants in the genes for voltage-gated calcium channel CaV1.1 (CACNA1S) and NaV1.4 (SCN4A). Patients with hypokalemic periodic paralysis may suffer from periodic paralysis alone, periodic paralysis co-existing with permanent weakness or permanent weakness alone. Hypokalemic periodic paralysis has been known to be associated with vacuolar myopathy for decades, and that vacuoles are a universal feature regardless of phenotype. Hence, we wanted to investigate the nature and cause of the vacuoles. Fourteen patients with the p.R528H variation in the CACNA1S gene was included in the study. Histology, immunohistochemistry and transmission electron microscopy was used to assess general histopathology, ultrastructure and pattern of expression of proteins related to muscle fibres and autophagy. Western blotting and real-time PCR was used to determine the expression levels of proteins and mRNA of the proteins investigated in immunohistochemistry. Histology and transmission electron microscopy revealed heterogenous vacuoles containing glycogen, fibrils and autophagosomes. Immunohistochemistry demonstrated autophagosomes and endosomes arrested at the pre-lysosome fusion stage. Expression analysis showed a significant decrease in levels of proteins an mRNA involved in autophagy in patients, suggesting a systemic effect. However, activation level of the master regulator of autophagy gene transcription, TFEB, did not differ between patients and controls, suggesting competing control over autophagy gene transcription by nutritional status and calcium concentration, both controlling TFEB activity. The findings suggest that patients with hypokalemic periodic paralysis have disrupted autophagic processing that contribute to the vacuoles seen in these patients.

Techniques for Transmission Electron Microscopy of Fiber-Matrix Interfaces in Aluminum Alloy-Boron Composites by Ion Erosio

1971 ◽  
Vol 29 ◽  
pp. 204-205
Author(s):  
G. G. Shaw
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Aluminum Alloy ◽  
Electron Beam ◽  
Pure Aluminum ◽  
Ion Milling ◽  
Transmission Electron ◽  
Fiber Matrix ◽  
Ion Erosion ◽  
Row Of Fibers

The morphology and composition of the fiber-matrix interface can best be studied by transmission electron microscopy and electron diffraction. For some composites satisfactory samples can be prepared by electropolishing. For others such as aluminum alloy-boron composites ion erosion is necessary.When one wishes to examine a specimen with the electron beam perpendicular to the fiber, preparation is as follows: A 1/8 in. disk is cut from the sample with a cylindrical tool by spark machining. Thin slices, 5 mils thick, containing one row of fibers, are then, spark-machined from the disk. After spark machining, the slice is carefully polished with diamond paste until the row of fibers is exposed on each side, as shown in Figure 1.In the case where examination is desired with the electron beam parallel to the fiber, preparation is as follows: Experimental composites are usually 50 mils or less in thickness so an auxiliary holder is necessary during ion milling and for easy transfer to the electron microscope. This holder is pure aluminum sheet, 3 mils thick.

Direct Observation of Heat Exchanger Deposits by Cross Sectioning

1967 ◽  
Vol 25 ◽  
pp. 364-365
Author(s):  
R. W. Anderson ◽  
D. L. Senecal
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Heat Exchanger ◽  
Direct Observation ◽  
Cross Sections ◽  
Preparation Method ◽  
Specimen Preparation ◽  
Flowing Water ◽  
Transmission Electron ◽  
Deposit Layer

A problem was presented to observe the packing densities of deposits of sub-micron corrosion product particles. The deposits were 5-100 mils thick and had formed on the inside surfaces of 3/8 inch diameter Zircaloy-2 heat exchanger tubes. The particles were iron oxides deposited from flowing water and consequently were only weakly bonded. Particular care was required during handling to preserve the original formations of the deposits. The specimen preparation method described below allowed direct observation of cross sections of the deposit layers by transmission electron microscopy.The specimens were short sections of the tubes (about 3 inches long) that were carefully cut from the systems. The insides of the tube sections were first coated with a thin layer of a fluid epoxy resin by dipping. This coating served to impregnate the deposit layer as well as to protect the layer if subsequent handling were required.

Sign in / Sign up

Export Citation Format

Share Document