Variations of plasti ds in pollen-derivated albino plants of rice

1986 ◽  
Vol 44 ◽  
pp. 196-197
Author(s):  
Y. R. Chen ◽  
Y. F. Huang ◽  
T. L. Lin
Keyword(s):  
Anther Culture ◽  
Membrane System ◽  
Concentric Ring ◽  
Starch Grains ◽  
Albino Plant ◽  
Albino Plants ◽  
Different Tissues

Pollen-derivated albino plants often occurred in anther culture and their percentages in few cases reached 100%. Clapham first observed the concentric ring of membrane system, plastoglobuli and starch grains in the plastids of albina barley. In mesophyllous cell of albino rice, the proplastid contained no ribosomes and its development was blocked at the stage of concentric membrane system. In the present study, plastids of different tissues of albino plant were examined.

scholarly journals The Role of Drought Stress on Anther Culture of Wheat (Triticum aestivum L.)

2010 ◽  
Vol 20 (1) ◽  
pp. 55-61 ◽  
Author(s):  
S. M. Shahinul Islam
Keyword(s):  
Triticum Aestivum ◽  
Drought Stress ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Plant Production ◽  
Albino Plant ◽  
Albino Plants ◽  
Embryo Yield ◽  
Embryo Regeneration

Drought stress was found to pose a significant effect on anther culture of wheat, namely Barkat, Kanchan and Pavon-76. Regeneration potentials of these varieties were determined by estimating the percentage of anther response, embryo induction, embryo regeneration and production of green and albino plants. It was observed that out of five treatments such as T1 (1 hr), T2 (3 hr), T3 (5 hr), T4 (7 hr) and T5 (9 hr) only T2 showed highest percentage of embryo yield and green plantlets. The T1 and T3 also gave significantly better results compared to the control. All the genotypes produced embryos and green plantlets and of them Barkat showed best performance followed by Kanchan and Pavon 76. Genotypes, under this study, produced green plants in addition to albinos but T4 and T5 showed three - fourfold higher albino plant production in comparison to treatments.  Key words: Anther culture, Drought stress, Haploids, Triticum aestivum D.O.I. 10.3329/ptcb.v20i1.5965 Plant Tissue Cult. & Biotech. 20(1): 55-61, 2010 (June)

scholarly journals Identification of QTLs associated with albino plant formation and some new facts concerning green versus albino ratio determinants in triticale (×Triticosecale Wittm.) anther culture

Euphytica ◽  
2015 ◽  
Vol 206 (1) ◽  
pp. 263-278 ◽  
Author(s):  
Monika Krzewska ◽  
Ilona Czyczyło-Mysza ◽  
Ewa Dubas ◽  
Gabriela Gołębiowska-Pikania ◽  
Iwona Żur
Keyword(s):  
Anther Culture ◽  
Albino Plant

scholarly journals Influence of test tube sealing on the morpho-anatomy and ultrastructure of leaves of Aechmea bromeliifolia (Bromeliaceae) grown in vitro

Rodriguésia ◽  
2020 ◽  
Vol 71 ◽  
Author(s):  
Elienai Candida e Silva ◽  
Sérgio Tadeu Sibov ◽  
Fernanda Cristina Alcantara dos Santos ◽  
Letícia Almeida Gonçalves
Keyword(s):  
Natural Ventilation ◽  
Stomatal Density ◽  
Membrane System ◽  
Test Tube ◽  
Microporous Membrane ◽  
Starch Grains ◽  
Sealing Material ◽  
Anatomy And Ultrastructure ◽  
Chloride Film

Abstract In vitro grown plants may have abnormal structural and physiological features. However, the type of the sealing material used in tissue culture may minimize such abnormalities. This study evaluates the influence of the type of sealing of test tubes on the anatomical and ultrastructural features of leaves of Aechemea bromeliifolia (Rudge) Baker (Bromeliaceae), an ornamental bromeliad native to Brazil, grown in vitro. Three types of sealing were used: rigid polypropylene cap (PC), polyvinyl chloride film (PVC), and PC coupled with a microporous membrane (PM). Seedlings germinated in a greenhouse were also studied for comparison. Plants grown in test tubes sealed with PM were more similar to those from the greenhouse, as far as the pattern of stomatal opening, the presence of starch grains, and the organization of the internal membrane system of the chloroplasts is concerned. Plants cultivated in test tubes sealed with PC had higher stomatal density and the chloroplasts had large areas without thylakoids in the stroma. Plants grown in test tubes sealed with PVC had few or no starch grains. These results suggest that microporous membrane used coupled with PC sealing provided natural ventilation, thus contributing to a better plant development.

Responsiveness to anther culture in cultivars and F1 crosses of spring wheat

1993 ◽  
Vol 73 (3) ◽  
pp. 777-783 ◽  
Author(s):  
P. Masojc ◽  
O. M. Lukow ◽  
R. I. H. McKenzie ◽  
N. K. Howes
Keyword(s):  
Anther Culture ◽  
Spring Wheat ◽  
Doubled Haploids ◽  
Green Plant ◽  
Donor Plant ◽  
Albino Plants ◽  
Ability To Regenerate ◽  
Embryoid Formation ◽  
N6 Medium ◽  
Regenerated Plant

Anther culturability of 43 cultivars and 6 F1 crosses representing different quality classes of spring wheat was studied using a glucose-containing, modified N6 medium (HNG). Generally high pollen embryoid formation (up to 111 embryoids per 100 anthers) was associated with lower green plant regeneration (up to 9.1 green plantlets per 100 embryoids) frequencies and a high proportion (63% on average) of albino plants. Anther response was found to be strongly affected by the genotype of the donor plants. Seven of the screened cultivars yielded more than one green regenerated plant per 100 anthers. The most responsive cultivars were Veery #2 (6.2), ST 6 (4.0), and Leader (3.6). No ability to regenerate green plantlets was shown by 10 of the genotypes. Anther responsiveness of F1 progenies as compared with the parental cultivars were different in each cross. Differences found between reciprocal crosses suggest that the cytoplasm of a donor plant may affect anther response. Haploid plants constituted 45% of the anther derived regenerants, while the remaining part was divided into 29% of spontaneous diploids and 26% of plants with abnormal chromosome numbers. Key words: Triticum aestivum, anther culture, doubled haploids, wheat

scholarly journals Anther culture properties of oat x wild red oat progenies and a search for RAPD markers associated with anther culture ability

2008 ◽  
Vol 13 (1-2) ◽  
pp. 151 ◽  
Author(s):  
E. KIVIHARJU ◽  
J. LAURILA ◽  
M. LEHTONEN
Keyword(s):  
Anther Culture ◽  
Avena Sativa ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Backcross Progeny ◽  
Avena Sterilis ◽  
Albino Plant ◽  
Parental Lines ◽  
Anther Culture Response ◽  
Genotype Effects

A study was carried out to improve anther culture ability of the non-responsive cultivated oat, Avena sativa L. cv. Puhti by introgressing favourable alleles from the responsive wild red oat, Avena sterilis L. acc. CAV 2648. Anther culture ability of these parental lines and F2 progenies of their cross and two backcrosses was tested. Genotype effects were significant on all anther culture traits measured. The number of anther culture derived embryo-like structures was highest in acc. CAV 2648, and the number of green regenerants from the Puhti × CAV 2648 progeny. Anther culture response was greatly reduced in backcross progeny and was least in cv. Puhti. Random amplified polymorphic DNA (RAPD) was used to test for marker associations with oat anther culture traits in a population of 38 F2 progenies. Two RAPD markers were putatively associated with improved production of green regenerants (one derived from acc. CAV 2648 and the other from cv. Puhti). One marker putatively associated with decreased albino plant regeneration (derived from acc. CAV 2648). These markers might be useful for selecting alleles for better anther culture ability among progeny of planned crosses. In addition, three markers, derived from acc. CAV 2648, were putatively associated with decreased anther culture response rates.;

scholarly journals Successful Regeneration and Characterization of Anther Derived Rice Hybrid Plants from O. Sativa L. X O. Rufipogon Griff.

1970 ◽  
Vol 5 (5) ◽  
pp. 14-18 ◽  
Author(s):  
Bindeshwar Prasad Sah ◽  
Raj Kumar Niroula
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Doubled Haploid Population ◽  
Albino Plants ◽  
Large Size ◽  
Hybrid Plants ◽  
Regenerated Plants ◽  
Scientific World ◽  
Haploid Population ◽  
Doubled Haploid Plants

Doubled haploid regenerated through anther culture of wide cross rice hybrids is one of the potential tools to produce various useful genetic and cytogenetic stocks for molecular studies. In this study, an interspecific hybrid derived from Oryza sativa and O. rufipogon was subjected to anther culture with the aim to regenerate doubled haploid population for their characterization. A total of 21 green and 31 albino plants were obtained from 1904 anthers of O. sativa cv. Himali x O. rufipogon. Six types of androclonal variants were identified based on morphology and panicle characteristics of regenerated plants. Among 21 plants, only six were found diploid with partially fertility. Despite the sterility and preponderance of wild traits, some of the plants showed useful traits such as increased in panicle and spikelet length, reduced shattering and awning, and large size anther as in O. rufipogon. Two of the regenerants were superior for panicle related traits over either parent. The genotyping study using SSR markers also revealed the substantial variation among regenerants. At least three allelic variants were recorded among individual plants based on our preliminary study. The regenerated doubled haploid plants showed large amount of useful variation both at phenotype and molecular level and they can be exploited to enrich the current rice gene pool. Key words: Anther culturability; Albino; Androclon; Calli; Meiosis; SSR marker. DOI: 10.3126/sw.v5i5.2649 Scientific World, Vol. 5, No. 5, July 2007 14-18

scholarly journals Androgenous ability of heterozygous wheat genotypes and cytological characteristics of green regenerants

Genetika ◽  
2006 ◽  
Vol 38 (2) ◽  
pp. 153-158
Author(s):  
Branka Ljevnaic ◽  
Ankica Kondic-Sipka ◽  
Borislav Kobiljski ◽  
Srbislav Dencic
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
Regeneration Ability ◽  
Cytological Analysis ◽  
Green Plants ◽  
Albino Plants ◽  
Cytological Characteristics

Androgenous and regeneration abilities of 8 heterozygous wheat (Triticum aestivum L.) genotypes were analyzed in anther culture. Cytological characteristics of green regenerants were also analyzed. Significant differences were found among the genotypes in their androgenous and regeneration abilities. The average androgenous capacity of all genotypes was 4.4% ranging from 0.7% (Prima/Hueguen) to 11.0% (NS173-98/NS164-98). The average callus yield was 8.7%. The genotype Banks/F53-70 produced the highest number of green plants (33 green plants), while the genotype Banks/Pobeda showed no green plant regeneration ability. The frequency of albino plants ranged between 0% (Banks/Rodna) and 5.3% (Anastasija/Radika). A total of 61 green and 61 albino plants were regenerated. The results of cytological analysis showed that, from the total number of regenerated green plants, 40.9% were haploids and 59.1% were spontaneous double haploids.

Deleted forms of plastid DNA in albino plants from cereal anther culture

1985 ◽  
Vol 9 (8) ◽  
pp. 671-678 ◽  
Author(s):  
Anil Day ◽  
T. H. Noel Ellis
Keyword(s):  
Anther Culture ◽  
Plastid Dna ◽  
Albino Plants

scholarly journals PRODUCTION OF HAPLOID ALBINO PLANTS OF AEGILOPS BY ANTHER CULTURE

1972 ◽  
Vol 47 (1) ◽  
pp. 61-63 ◽  
Author(s):  
MIKIO KIMATA ◽  
SADAO SAKAMOTO
Keyword(s):  
Anther Culture ◽  
Albino Plants
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