scholarly journals Anther culture properties of oat x wild red oat progenies and a search for RAPD markers associated with anther culture ability

2008 ◽  
Vol 13 (1-2) ◽  
pp. 151 ◽  
Author(s):  
E. KIVIHARJU ◽  
J. LAURILA ◽  
M. LEHTONEN
Keyword(s):  
Anther Culture ◽  
Avena Sativa ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Backcross Progeny ◽  
Avena Sterilis ◽  
Albino Plant ◽  
Parental Lines ◽  
Anther Culture Response ◽  
Genotype Effects

A study was carried out to improve anther culture ability of the non-responsive cultivated oat, Avena sativa L. cv. Puhti by introgressing favourable alleles from the responsive wild red oat, Avena sterilis L. acc. CAV 2648. Anther culture ability of these parental lines and F2 progenies of their cross and two backcrosses was tested. Genotype effects were significant on all anther culture traits measured. The number of anther culture derived embryo-like structures was highest in acc. CAV 2648, and the number of green regenerants from the Puhti × CAV 2648 progeny. Anther culture response was greatly reduced in backcross progeny and was least in cv. Puhti. Random amplified polymorphic DNA (RAPD) was used to test for marker associations with oat anther culture traits in a population of 38 F2 progenies. Two RAPD markers were putatively associated with improved production of green regenerants (one derived from acc. CAV 2648 and the other from cv. Puhti). One marker putatively associated with decreased albino plant regeneration (derived from acc. CAV 2648). These markers might be useful for selecting alleles for better anther culture ability among progeny of planned crosses. In addition, three markers, derived from acc. CAV 2648, were putatively associated with decreased anther culture response rates.;

Potential of trispecies bridge crosses and random amplified polymorphic DNA markers for introgression of Medicago daghestanica and M. pironae germplasm into alfalfa (M. sativa)

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 594-601 ◽  
Author(s):  
T. J. McCoy ◽  
C. S. Echt
Keyword(s):  
Embryo Culture ◽  
Interspecific Hybrids ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Embryo Rescue ◽  
Backcross Progeny ◽  
Culture Technique ◽  
Randomly Amplified Polymorphic Dna ◽  
Bivalent Chromosome ◽  
Diploid Level

This report describes the production and cytology of the first interspecific hybrids between cultivated alfalfa (Medicago sativa L.) at the diploid level (2n = 2x = 16) and the diploid (2n = 2x = 16) perennial species M. daghestanica and M. pironae. An ovule–embryo culture technique was required to rescue hybrid embryos and all hybrids were diploid. Predominately bivalent chromosome pairing was observed at meiotic metaphase. All F1 hybrids were male and female sterile and no species backcross progeny could be produced. We discovered that trispecies hybrids could be efficiently recovered via crossing diploid F1 interspecific hybrids of M. sativa × M. rupestris with either M. daghestanica or M. pironae. Ovule–embryo culture was also required to recover these trispecies hybrids with recovery efficiency of trispecies hybrids about 10 times greater than for bispecies hybrids. Most chromosomes paired as bivalents in the trispecies hybrids. Importantly, progeny can be recovered from crossing the trispecies hybrids with M. sativa. Therefore, the M. sativa × M. rupestris hybrids provide a bridge cross to potential introgression of M. daghestanica or M. pironae germplasm. Analysis of randomly amplified polymorphic DNA (RAPD) markers in the trispecies hybrids indicates that RAPD markers offer considerable potential for assaying germplasm introgression following complex hybridizations of the type reported here.Key words: randomly amplified polymorphic DNA, Medicago interspecific hybrids, embryo rescue.

RAPD analysis of seed purity in a commercial hybrid cabbage (Brassica oleracea var. capitata) cultivar

Genome ◽  
2000 ◽  
Vol 43 (2) ◽  
pp. 317-321 ◽  
Author(s):  
Phillip A Crockett ◽  
Prem L Bhalla ◽  
C K Lee ◽  
Mohan B Singh
Keyword(s):  
Brassica Oleracea ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Arbitrary Primers ◽  
Purity Testing ◽  
Ungerminated Seed ◽  
Hybrid Seeds ◽  
Seed Purity Test ◽  
Parental Lines

The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in a commercial F1-hybrid cabbage (Brassica oleracea var. capitata) cultivar is demonstrated. Genomic DNA isolated from single ungerminated seed was found to be suitable for RAPD analysis. DNA from F1-hybrid and its parental lines was subjected to RAPD screening with 36 random decamer arbitrary primers. A total of 241 scorable products were observed with 54 (22%) being polymorphic. The RAPD data showed that the parental lines of this commercial cabbage cultivar were not very closely related. Two primers were chosen for purity testing of the F1-hybrid seeds. The sib (inbred seed; seed from self-pollination of parental lines) contamination results obtained by RAPD analysis were comparable to the commonly used grow-out trial and isozyme analysis, hence showing that RAPD analysis can be used for seed purity testing of commercial hybrid cabbage seeds. Key words: Brassica, cabbage, RAPD, seed purity test, F1-hybrid seed.

Generation of SNP markers for short straw in oat (Avena sativa L.)

Genome ◽  
2006 ◽  
Vol 49 (3) ◽  
pp. 282-287 ◽  
Author(s):  
Pirjo Tanhuanpää ◽  
Ruslan Kalendar ◽  
Jaana Laurila ◽  
Alan H Schulman ◽  
Outi Manninen ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Avena Sativa ◽  
Marker Assisted Selection ◽  
Rapd Markers ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Snp Markers ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Dwarfing Gene

Short straw is a desired trait in oat germplasm (Avena sativa L.). Marker-assisted selection, a key tool for achieving this objective, is limited by the presence and number of available markers. Here, we have attempted to develop markers sufficiently linked to a gene specifying short straw so that marker-assisted selection could be applied. Bulked-segregant analysis was used to identify anonymous PCR-based markers associated with the dwarfing gene Dw6 in an F2 population from the cross between A. sativa 'Aslak' and A. sativa 'Kontant'. One random amplified polymorphic DNA (RAPD) and 1 retrotransposon-microsatellite amplified polymorphism (REMAP) marker were found to be associated with height. These were converted into codominant single-nucleotide polymorphism (SNP) markers. The SNP–REMAP and the SNP–RAPD markers were located 5.2 and 12.6 cM from Dw6, respectively. They can be used in future efforts both to enhance oat germplasm by application of molecular markers and to determine the nature of the gene through positional cloning.Key words: Avena sativa, short straw, marker-assisted selection, RAPD, REMAP, SNP.

scholarly journals The effect of genotype on anther culture response of cultivated and wild oats

1998 ◽  
Vol 7 (3) ◽  
pp. 409-422 ◽  
Author(s):  
Elina Kiviharju ◽  
Matti Puolimatka ◽  
Marketta Saastamoinen ◽  
Simo Hovinen ◽  
Eija Pehu
Keyword(s):  
Anther Culture ◽  
Interspecific Crosses ◽  
Induction Medium ◽  
Regeneration Ability ◽  
Dichlorophenoxyacetic Acid ◽  
Wild Oat ◽  
Avena Sterilis ◽  
Naked Oat ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Anther Culture Response

Anther culture ability was tested for 44 oat (Avena sativa L.), six naked oat (A. sativa L., naked type) and 15 wild oat (Avena sterilis L.) genotypes, in addition to progeny of five intraspecific crosses of A. sativa and two interspecific crosses of A. sativa x A. sterilis. Anther culture response was affected considerably by genotype. Thirty one oat genotypes responded by callus growth on induction medium and seven of them produced embryo structures, two of the lines consistently. All naked oat genotypes produced embryo structures. Embryo production rates for the wild oat lines were comparable with those for the naked oat genotypes, and higher than for oat: 13 of the 15 genotypes tested produced embryo structures. Plant regeneration was possible only from wild oat. The regeneration ability was inherited in the progeny of the A. sativa x A. sterilis cross cv. Puhti x CAV 2648. The response of anthers of oat genotypes was inhibited by auxin on the induction medium, while naked oat, wild oat and A. sativa x A. sterilis crosses responded better on a medium containing 2,4-dichlorophenoxyacetic acid.

EVALUATION OF PTYCHOBOTHRIDEAN CESTODES USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FROM FRESH WATER FISHES IN GODAVARI BASIN (M.S.) INDIA

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
SUNITA BORDE ◽  
ASAWARI FARTADE ◽  
AMOL THOSAR ◽  
RAHUL KHAWAL
Keyword(s):  
Fresh Water ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Band Pattern ◽  
Genomic Variation ◽  
Polymorphic Band ◽  
Rapd Profile ◽  
Band Size ◽  
Pcr Product ◽  
The Common

Ptychobothridean genera like Senga and Circumoncobothrium are the common parasites of fresh water fishes. The genotypic study of these parasites was taken by RAPD. The RAPD profile of these two parasites were not similar to each other as depicted by the band pattern in picture. These results suggest the presence of inter-specific polymorphism among cestode parasites of two different genera for RAPD analysis. The present study demonstrated that genetic differentiation of cestode parasites could be accomplished on the basis of genomic variation with polymorphic band pattern using RAPD. All the detected bands (PCR product) were polymorphic and band size ranged from 500-5000 bp in length. The RAPD of profiles using GBO-31, GBO-32, GBO-33, GBO-34, GBO-35 and GBO-36. Primers were able to characterize inter-specific polymorphism among the two genus ( Senga and Circumoncobothrium ). Genetic analysis suggests that Senga and Circumoncobothrium show genetic diversity with respect to RAPD patterns using all the six primers used for the present study. The genetic distance between the analyzed genuses ranged from 0.14 to 0.80. The differentiation of the two parasites on the basis of genetic markers could greatly facilitate study on the biology of these parasites.

scholarly journals Detection and analysis of genetic variation in Salicornieae (Chenopodiaceae) using random amplified polymorphic DNA (RAPD) markers

Taxon ◽  
1995 ◽  
Vol 44 (1) ◽  
pp. 53-63 ◽  
Author(s):  
T. Luque ◽  
C. Ruiz ◽  
J. Avalos ◽  
I. L. Calderón ◽  
M. E. Figueroa
Keyword(s):  
Genetic Variation ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna

scholarly journals DNA profiling of commercial chilli pepper (Capsicum annuum L.) varieties using random amplified polymorphic DNA (RAPD) markers

2013 ◽  
Vol 12 (30) ◽  
pp. 4730-4735 ◽  
Author(s):  
Prasad Bylla ◽  
Gulab Khan R ◽  
Radha T ◽  
Ravi Ch ◽  
Venkataiah P ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Dna Profiling ◽  
Chilli Pepper ◽  
Capsicum Annuum L

Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 995-999 ◽  
Author(s):  
H I Amadou ◽  
P J Bebeli ◽  
P J Kaltsikes
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Bambara Groundnut ◽  
International Institute ◽  
Tropical Agriculture ◽  
Vigna Subterranea ◽  
Ibadan Nigeria

Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm using 25 African accessions from the collection in the International Institute for Tropical Agriculture, Ibadan, Nigeria. Fifty random decamer primers were screened to assess their ability to detect polymorphism in bambara; 17 of them were selected for this study. Considerable genetic diversity was found among the V. subterranea accessions studied. The relationships among the 25 accessions were studied by cluster analysis. The dendrograms showed two main groups of accessions mainly along the lines of their geographic origin. It is concluded that RAPD can be used for germplasm classification in bambara groundnut and hence for improving this crop.Key words: germplasm, PCR, RAPD, Vigna subterranea.

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