The ultrastructure of the molluscum contagiosum lesion

Author(s):  
Veronika Burmeister ◽  
W.B. Shelley

Molluscum contagiosum is unique among the viral induced growths of the skin. Unlike other viral tumors, the molluscum contagiosum lesion is a mass made up of myriads of free, discrete virions. It is not a proliferating growth of epidermal keratinocytes such as one observes in the common wart. There is no better way to perceive the nature of this tumor mass than by scanning electron microscopy.Skin lesions of molluscum contagiosum were excised from patients and immediately fixed in 2.5% glutaraldehyde. After fixation the specimens were rinsed in Millonig’s Phosphate buffer. The entire lesion was than cross-sectioned with a razor blade, postfixed in 1% OsO4, rinsed again in buffer, dehydrated in grading ETOH, critical point dried, positioned on an aluminum stub and gold-coated. Examination was made using a JEOL SEM (JSM-35).

1971 ◽  
Vol 8 (11) ◽  
pp. 1487-1491 ◽  
Author(s):  
D. A. Walker

Investigations by scanning electron microscopy have revealed that ingestion of Rosalina floridana (Cushman) and Quinqueloculina seminulum (Linné) (Foraminiferida) by the common periwinkle Littorina littorea Linné results in severe etching of the surface veneer in the rotalids studied, and removal of the surface veneer and partial dissolution of the underlying tabular layer of calcite in the miliolids examined. The acidic nature of the digestive juices is suggested as the agent responsible for this phenomenon. Observations of test wall construction is compared to current models of calcite secretion.


2004 ◽  
Vol 52 (3) ◽  
pp. 285 ◽  
Author(s):  
G. Prenner

The floral development of Daviesia cordata Smith is studied by the use of scanning electron microscopy. This is the first study of a member of Mirbelieae. Although organ initiation in Papilionoideae is said to be almost uniformly unidirectional from the abaxial to the adaxial side, the presented floral development shows striking differences from this mode. Sepals, petals and the antepetalous stamens are initiated in simultaneous whorls, which is seen as a consequence of harmonisation of the plastochrons within the whorls. The antesepalous stamens are initiated unidirectional from the adaxial to the abaxial side, which is the reversed direction of the common mode of Papilionoideae. This is the first record of reversed unidirectionality in Papilionoideae, which can be linked with isolated findings in Caesalpinioideae and Mimosoideae. Concerning developmental aspects, the results seem to link the papilionoid flower closer to those of Caesalpinioideae and Mimosoideae. Further developmental studies are necessary to broaden the data matrix for a detailed phylogenetic analysis.


2013 ◽  
Vol 88 (1) ◽  
pp. 90-93 ◽  
Author(s):  
Hiram Larangeira de Almeida Jr ◽  
Martha Oliveira Abuchaim ◽  
Maiko Abel Schneide ◽  
Leandra Marques ◽  
Luis Antônio Suíta de Castro

Molluscum contagiosum is a disease caused by a poxvirus. It is more prevalent in children up to 5 years of age. There is a second peak of incidence in young adults. In order to examine its ultrastructure, three lesions were curetted without disruption, cut transversely with a scalpel, and routinely processed for scanning electron microscopy (SEM). The oval structure of molluscum contagiosum could be easily identified. In its core, there was a central umbilication and just below this depression, there was a keratinized tunnel. Under higher magnification, a proliferation similar to the epidermis was seen. Moreover, there were areas of cells disposed like a mosaic. Under higher magnification, rounded structures measuring 0.4 micron could be observed at the end of the keratinized tunnel and on the surface of the lesion.


1998 ◽  
Vol 163 (1) ◽  
pp. 31-38 ◽  
Author(s):  
S. Thongpila ◽  
S. Rojananeungnit ◽  
P. Chunhabundit ◽  
C. Cherdchu ◽  
A. Samritthong ◽  
...  

1998 ◽  
Vol 76 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Gabriele Uhl ◽  
Fritz Vollrath

The genital morphology of the female is assumed to control the pattern of sperm priority. Spiders are divided roughly along phylogenetic lines into haplogyne and entelegyne types, the principal difference being in female genital morphology (cul-de-sac and conduit types of spermathecae). Nephila edulis is an entelegyne spider and we studied the genital morphology of both sexes by means of scanning electron microscopy and semithin sectioning. In the female, the copulatory ducts leading to the spermatheca were much longer and more differentiated than the fertilization duct. We question the common assumption that possession of genitalia of the conduit type with separate copulatory and fertilization ducts will automatically lead to first-male sperm priority because in our typical entelegyne species, the copulatory ducts were similar in arrangement to the combined copulatory/fertilization ducts in genitalia of the typical cul-de-sac type.


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