scholarly journals Epidemiological survey and sequence information analysis of swine hepatitis E virus in Sichuan, China

2018 ◽  
Vol 147 ◽  
Author(s):  
Y. Y. Li ◽  
Z. W. Xu ◽  
X. J. Li ◽  
S. Y. Gong ◽  
Y. Cai ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Biological Evolution ◽  
Hepatitis E ◽  
Epidemiological Survey ◽  
Sichuan Province ◽  
Positive Sample ◽  
Sequence Information ◽  
Large White ◽  
Positive Rate ◽  
Swine Hepatitis

AbstractHepatitis E is an important zoonosis that is prevalent in China. Hepatitis E virus (HEV) is a pathogen that affects humans and animals and endangers public health in China. In this study, the detection of HEV epidemics in swine in Sichuan Province, China, was carried out by nested real-time PCR. A total of 174 stool samples and 160 bile samples from swine in Sichuan Province were examined. In addition, software was used to analyse the biological evolution of HEV. The results showed that within 2 years of swine HEV (SHEV) infection in China, SHEV was first detected in Sichuan Province. HEV was endemic in Sichuan; the positive rate for pig farms was 11.1%, and the total positive sample rate was 10.5%. The age of swine with the highest positive rate (17.9%) was 5–9 weeks. The examined swine species in order of highest to lowest HEV infection rates were Chenghua pig, Large White, Duroc, Pietrain, Landrace and Hampshire. Nucleotide and amino acid sequence analysis showed that the HEV epidemic in swine in Sichuan Province was related to genotype IV, which had the highest homology to HEV in Beijing. Sichuan strains have greater variation than Chinese representative strains, which may indicate the presence of new HEV strains.

scholarly journals Seasonal pattern of hepatitis E virus prevalence in swine in two different geographical areas of China

2013 ◽  
Vol 141 (11) ◽  
pp. 2403-2409 ◽  
Author(s):  
Y. H. LU ◽  
H. Z. QIAN ◽  
A. Q. HU ◽  
X. QIN ◽  
Q. W. JIANG ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Seasonal Pattern ◽  
Eastern China ◽  
Geographical Area ◽  
Hepatitis E ◽  
Southwestern China ◽  
Seasonal Patterns ◽  
Virus Prevalence ◽  
Swine Hepatitis ◽  
A Minor

SUMMARYWe studied seasonal patterns of swine hepatitis E virus (HEV) infection in China. From 2008 to 2011, 4200 swine bile specimens were collected for the detection of HEV RNA. A total of 92/2400 (3·83%) specimens in eastern China and 47/1800 (2·61%) specimens in southwestern China were positive for HEV. Seasonal patterns differing by geographical area were suggested. In eastern China, the major peak of HEV RNA prevalence was during March–April, with a minor peak during September–October, and a dip during July–August. In southwestern China, the peak was during September–October and the dip during March–April. The majority of subtype 4a cases (63/82, 76·83%) were detected in the first half of the year, while the majority of subtype 4b cases (26/29, 89·66%) were concentrated in the second half of the year, suggesting that different subtypes contribute to different peaks. Our results indicate that the distribution of HEV subtypes is associated with seasonal patterns.

Experimental infection of mongolian gerbils by a genotype 4 strain of swine hepatitis E virus

2009 ◽  
Vol 81 (9) ◽  
pp. 1591-1596 ◽  
Author(s):  
Wengui Li ◽  
Quan Sun ◽  
Ruiping She ◽  
Decheng Wang ◽  
Xinhui Duan ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Mongolian Gerbils ◽  
Genotype 4 ◽  
Swine Hepatitis

scholarly journals Induction of antibody and interferon-γ production in mice immunized with virus-like particles of swine hepatitis E virus

2014 ◽  
Vol 15 (4) ◽  
pp. 575 ◽  
Author(s):  
Young-Jo Song ◽  
Woo-Jung Park ◽  
Seul-Kee Lee ◽  
Joong-Bok Lee ◽  
Seung-Yong Park ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Interferon Γ ◽  
Virus Like Particles ◽  
Swine Hepatitis

scholarly journals Detection of Human Food-Borne and Zoonotic Viruses on Irrigated, Field-Grown Strawberries

2012 ◽  
Vol 78 (10) ◽  
pp. 3763-3766 ◽  
Author(s):  
Julie Brassard ◽  
Marie-Josée Gagné ◽  
Mylène Généreux ◽  
Caroline Côté
Keyword(s):  
Irrigation Water ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Human Food ◽  
Food Borne ◽  
Zoonotic Viruses ◽  
Swine Hepatitis

ABSTRACTThis study evaluated the presence of pathogenic human and zoonotic viruses on irrigated, field-grown strawberries. Norovirus genogroup I, rotavirus, and swine hepatitis E virus genogroup 3 were detected on strawberries, and irrigation water is suspected as the contamination origin.

scholarly journals Molecular characterization of swine hepatitis E virus from Southeastern Brazil

2007 ◽  
Vol 38 (4) ◽  
pp. 693-698 ◽  
Author(s):  
Helder Henrique Paiva ◽  
Valentina Tzaneva ◽  
Rodrigo Haddad ◽  
Jonny Yokosawa
Keyword(s):  
Molecular Characterization ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Southeastern Brazil ◽  
Swine Hepatitis

scholarly journals Synthetic Peptides Containing Three Neutralizing Epitopes of Genotype 4 Swine Hepatitis E Virus ORF2 induced Protection against Swine HEV Infection in Rabbit

Vaccines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 178
Author(s):  
Yiyang Chen ◽  
Tianxiang Chen ◽  
Yuhang Luo ◽  
Jie Fan ◽  
Meimei Zhang ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Keyhole Limpet Hemocyanin ◽  
Hepatitis E ◽  
Genotype 4 ◽  
Virus Shedding ◽  
Fecal Shedding ◽  
Orf2 Protein ◽  
Swine Hepatitis ◽  
Phosphate Buffered Saline ◽  
Neutralizing Epitopes

Genotype 4 hepatitis E virus (HEV) is a zoonotic pathogen transmitted to humans through food and water. Previously, three genotype 4 swine HEV ORF2 peptides (407EPTV410, 410VKLYTS415, and 458PSRPF462) were identified as epitopes of virus-neutralizing monoclonal antibodies that partially blocked rabbit infection with swine HEV. Here, individual and tandem fused peptides were synthesized, conjugated to keyhole limpet hemocyanin (KLH), then evaluated for immunoprotection of rabbits against swine HEV infection. Forty New Zealand White rabbits were randomly assigned to eight groups; groups 1 thru 5 received three immunizations with EPTV-KLH, VKLYTS-KLH, PSRPF-KLH, EPTVKLYTS-KLH, or EPTVKLYTSPSRPF-KLH, respectively; group 6 received truncated swine HEV ORF2 protein (sp239), and group 7 received phosphate-buffered saline. After an intravenous swine HEV challenge, all group 7 rabbits exhibited viremia and fecal virus shedding by 2–4 weeks post challenge (wpc), seroconversion by 4–9 wpc, elevated alanine aminotransferase (ALT) at 2 wpc, and severe liver lymphocytic venous periphlebitis. Only 1–2 rabbits/group in groups 1–4 exhibited delayed viremia, fecal shedding, seroconversion, increased ALT levels, and slight liver lymphocytic venous periphlebitis; groups 5–6 showed no pathogenic effects. Collectively, these results demonstrate that immunization with a polypeptide containing three genotype 4 HEV ORF2 neutralizing epitopes completely protected rabbits against swine HEV infection.

scholarly journals Detection of swine hepatitis E virus in the porcine hepatic lesion in Jeju Island

2007 ◽  
Vol 8 (1) ◽  
pp. 51 ◽  
Author(s):  
Song-Hak Lee ◽  
Sang-Chul Kang ◽  
Dae-Yong Kim ◽  
Jong-Hee Bae ◽  
Jae-Hoon Kim
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Jeju Island ◽  
Hepatic Lesion ◽  
Swine Hepatitis
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