Spectral sensitivity of the feedback signal from horizontal cells to cones in goldfish retina

1998 ◽  
Vol 15 (5) ◽  
pp. 799-808 ◽  
Author(s):  
D.A. KRAAIJ ◽  
M. KAMERMANS ◽  
H. SPEKREIJSE
Keyword(s):  
Spectral Sensitivity ◽  
Horizontal Cell ◽  
Horizontal Cells ◽  
Feedback Signal ◽  
Cell Systems ◽  
Long Wavelength ◽  
Cell Recording ◽  
Cone System ◽  
Goldfish Retina ◽  
Uv Range

The spectral sensitivity of cones in isolated goldfish retina was determined with whole-cell recording techniques. Three spectral classes of cones were found with maximal sensitivities around 620 nm, 540 nm, and 460 nm. UV-cones were not found because our stimulator did not allow effective stimulation in the UV range. The spectral sensitivity of the cones closely matched the cone photopigment absorption spectra at the long wavelength side of the spectrum, but deviated significantly at shorter wavelengths. Surround stimulation induced an inward current in cones due to feedback from horizontal cells. The spectral sensitivity of this feedback signal was determined in all three cone classes and found to be broader than the spectral sensitivity of the cones recorded from, and to be spectrally nonopponent. These data are consistent with a connectivity scheme between cones and horizontal cells in which the three horizontal cell systems feed back to all cone systems and in which all horizontal cell systems receive input from more than one cone system.

Nitric oxide and cGMP modulate retinal glutamate receptors

1996 ◽  
Vol 76 (4) ◽  
pp. 2307-2315 ◽  
Author(s):  
D. G. McMahon ◽  
L. V. Ponomareva
Keyword(s):  
Nitric Oxide ◽  
Glutamate Receptors ◽  
Horizontal Cell ◽  
Synaptic Function ◽  
Horizontal Cells ◽  
Glutamate Excitotoxicity ◽  
No Donors ◽  
Receptor Modulation ◽  
Cell Recording ◽  
Endogenous Nitric Oxide

1. In the retina, as in other regions of the vertebrate central nervous system, glutamate receptors mediate excitatory chemical synaptic transmission and are a critical site for the regulation of cellular communication. In this study, retinal horizontal cells from the hybrid less were dissociated in cell culture, voltage clamped by the whole cell recording technique, and the currents evoked by application of excitatory amino acids recorded. 2. Responses to glutamate and its agonist kainate were reduced by approximately 50% in the presence of the nitric oxide (NO) donors sodium nitroprusside and S-nitroso-N-acetylpenicillamine. The effect of these compounds was blocked by the NO scavenger hemoglobin. 3. This effect of NO donors on kainate currents could be mimicked by the application of a membrane permeable guanosine 3',5'-cyclic monophosphate (cGMP) analogue, 8-Br-cGMP. The NO effect was also blocked by application of the guanylate cyclase inhibitor LY-83583, and by a protein kinase G inhibitor peptide. 4. In H1-type horizontal cells, stimulation of endogenous nitric oxide synthase with L-arginine reduced kainate responses, whereas application of D-arginine had no effect. 5. This receptor modulation mechanism may act in concert with other pre- and postsynaptic mechanisms to modify horizontal cell synaptic function according to the adaptational state of the retina and also may protect horizontal cells from glutamate excitotoxicity.

3H-adenosine uptake selectively labels rod horizontal cells in goldfish retina

1997 ◽  
Vol 14 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Keith M. Studholme ◽  
Stephen Yazulla
Keyword(s):  
Staining Pattern ◽  
Outer Nuclear Layer ◽  
Horizontal Cell ◽  
Horizontal Cells ◽  
The Other ◽  
Axon Terminals ◽  
Adenosine Uptake ◽  
Double Label ◽  
Injection Methods ◽  
Goldfish Retina

AbstractThere are four types of horizontal cell in the goldfish retina, three cone- and one rod-type. The neurotransmitter of only one type, the H1 (cone) horizontal cell, has been identified as GABA. 3H-adenosine uptake was examined as a possible marker for the other classes of horizontal cell. Isolated goldfish retinae were incubated in 3H-adenosine (10–40 μCi) in HEPES-buffered saline for 30 min, then fixed, embedded in plastic, and processed for light-microscopic autoradiography (ARG). For double-label immuno/ARG studies, l-μm-thick sections were processed for GABA postembed immunocytochemistry, then for ARG. 3H-adenosine uptake was localized to cone photoreceptors, presumed precursor cells in the proximal outer nuclear layer, and to a single, continuous row of horizontal cell bodies in the inner nuclear layer. No uptake was localized to the region of horizontal cell axon terminals. 3H-adenosine uptake did not colocalize with GABA-IR in H1 horizontal cells, but it did colocalize with adenosine deaminase immunoreactivity. It is concluded that 3H-adenosine uptake selectively labels rod horizontal cells in the goldfish retina based on position and staining pattern, which are similar to rod horizontal cells stained by Golgi or HRP injection methods. The use of 3H-adenosine uptake may provide a useful tool to study other properties of rod horizontal cells (i.e. development) as well as provide clues as to the transmitter used by these interneurons.

Background-induced flicker enhancement in cat retinal horizontal cells. I. Temporal and spectral properties

1990 ◽  
Vol 64 (2) ◽  
pp. 313-325 ◽  
Author(s):  
R. Pflug ◽  
R. Nelson ◽  
P. K. Ahnelt
Keyword(s):  
Light Adaptation ◽  
Dynamic Range ◽  
Horizontal Cell ◽  
Plexiform Layer ◽  
Horizontal Cells ◽  
Enhancement Effect ◽  
Long Wavelength ◽  
Flicker Response ◽  
Response Enhancement ◽  
Cone Signal

1. Dim backgrounds can enhance small-spot flicker responses of cat retinal horizontal cells by a factor of 2 or more. 2. Intracellular marking with horseradish peroxidase (HRP) reveals that this enhancement effect occurs in--but is not necessarily limited to--the cone-connected, A-type horizontal cell. 3. Flicker amplitudes decrease over a frequency range from 3 to 36 Hz of square-wave photic stimulation. There is little evidence of flicker-response enhancement at 3 Hz. Flicker-response enhancement is typically 2-6 times larger at 35 than at 6 Hz. 4. Inspection of flicker waveforms indicates both a scaling-up of response signals with backgrounds and a distortion composed of 2- to 5-ms-latency decrease, expressed primarily within a quick component of OFF-repolarization. 5. Flicker enhancement first increases as a function of background irradiance and then decreases. The increasing limb has the dynamic range and spectral sensitivity of cat rods (507-nm peak). Enhancement is maintained during rod after-effects. The decreasing limb of the background-versus-intensity function results from light adaptation of cat, long-wavelength (red) cones. 6. The flicker responses themselves peak spectrally at approximately 555 nm and reflect only the activity of cat long-wavelength (red) cones, without evidence of intermixing of other photoreceptor mechanisms. 7. Thus within the first synaptic layer of the cat visual system, rod signals interact with the flicker responses of red cones, both increasing cone-signal amplitudes and modifying cone-signal waveforms. 8. The results are closely analogous to "suppressive rod-cone interaction" (SRCI) as described in human psychophysics. 9. An outer-plexiform-layer circuit involving rods, horizontal cells and cones may mediate rod-induced enhancement of cone flicker. This being the case, notions of horizontal-cell feedback interactions with cones may have to be modified and extended. A specific feedback model is elaborated in the companion paper.

Tetrachromatic input to turtle horizontal cells

2001 ◽  
Vol 18 (5) ◽  
pp. 759-765 ◽  
Author(s):  
Y. ZANA ◽  
D.F. VENTURA ◽  
J.M. de SOUZA ◽  
R.D. DeVOE
Keyword(s):  
Spectral Sensitivity ◽  
Horizontal Cell ◽  
Sensitivity Function ◽  
Horizontal Cells ◽  
Chromatic Adaptation ◽  
Morphological Evidence ◽  
Cone Type ◽  
Response Method ◽  
Uv Cone ◽  
Spectral Sensitivity Function

Recent physiological experiments support behavioral and morphological evidence for a fourth type of cone in the turtle retina, maximally sensitive in the ultraviolet (UV). This cone type has not yet been included in the models proposed for connectivity between cones and horizontal cells. In this study, we examined the inputs of UV, S, M, and L cones to horizontal cells. We used the high-resolution Dynamic Constant Response Method to measure the spectral sensitivity of horizontal cells without background light and after adaptation to UV, blue (B), green (G), and red (R) light. We concluded the following: (1) Tetrachromatic input to a Y/B horizontal cell was identified. The spectral-sensitivity curves of the cell in three of the adaptation conditions were well represented by L-, M-, and S-cone functions. Adaptation to blue light revealed a peak at 372 nm, the same wavelength location as that determined behaviorally in the turtle. A porphyropsin template could be closely fitted to the sensitivity band in that region, strong evidence for input from a UV cone. (2) The spectral-sensitivity functions of R/G horizontal cells were well represented by the L- and M-cone functions. There was no indication of UV- or S-cone inputs into these cells. (3) The spectral sensitivities of the monophasic horizontal cells were dominated by the L cone. However, the shape of the spectral-sensitivity function depended on the background wavelength, indicating secondary M-cone input. Connectivity models of the outer retina that predict input from all cone types are supported by the finding of tetrachromatic input into Y/B horizontal cells. In contrast, we did not find tetrachromatic input to R/G and monophasic horizontal cells. Chromatic adaptation revealed the spectral-sensitivity function of the turtle UV cone peaking at 372 nm.

Contribution of the retinal ON channels to scotopic and photopic spectral sensitivity

1989 ◽  
Vol 3 (3) ◽  
pp. 225-239 ◽  
Author(s):  
Earl L. Smith ◽  
Ronald S. Harwerth ◽  
M.L.J. Crawford ◽  
Gary C. Duncan
Keyword(s):  
Spectral Sensitivity ◽  
Visual Information ◽  
Visible Spectrum ◽  
Bipolar Cells ◽  
Long Wavelength ◽  
Rod System ◽  
Cone System ◽  
Organizational Differences ◽  
Functional Inactivation ◽  
Rod And Cone Systems

AbstractVisual information encoded by the middle-wavelength-sensitive (MWS) and long-wavelength-sensitive (LWS) cones in the primate retina are processed by both depolarizing (ON) and hyperpolarizing (OFF) bipolar cells. In contrast, signals from the short-wavelength-sensitive (SWS) cones and dark-adapted rod photoreceptors are thought to be carried almost exclusively by ON bipolar cells (Gouras & Evers, 1985). Consequently, it would be expected that functional inactivation of the retinal ON channels at the bipolar cell level would produce selective deficits in visual functions mediated by rods and SWS cones. We have examined this hypothesis by injecting rhesus monkeys with 2-amino-4-phosphonobutyric acid (APB), a pharmacological agent that reduces the responsiveness of retinal ON neurons, and psychophysically measuring the changes in spectral sensitivities. Under adaptation conditions that isolated rod function, APB caused, as expected, a substantial loss in rod-mediated spectral sensitivity. However, under photopic conditions, cone-mediated spectral sensitivity, including that associated with the SWS cones, was relatively unaffected. These results demonstrate distinct organizational differences between the rod and cone systems; specifically, they indicate that the rod system is more dependent upon retinal ON channels than the cone system. Our failure to find a selective visual deficit related to SWS cone function under photopic viewing conditions suggests that the OFF system can mediate stimulus detection throughout the visible spectrum and that the ability of the OFF system to process signals from the SWS cones has been underestimated.

scholarly journals The eel retina. Receptor classes and spectral mechanisms.

1978 ◽  
Vol 71 (2) ◽  
pp. 123-138 ◽  
Author(s):  
J Gordon ◽  
R M Shapley ◽  
E Kaplan
Keyword(s):  
Spectral Sensitivity ◽  
Light And Electron Microscopy ◽  
Preliminary Evidence ◽  
Horizontal Cells ◽  
Color Processing ◽  
Rods And Cones ◽  
Isolated Retina ◽  
Cell Recording ◽  
Opponent Color ◽  
Vitamin A2

Light and electron microscopy revealed that there are both rods and cones in the retina of the eel Anguilla rostrata. The rods predominate with a rod to cone ratio of 150:1. The spectral sensitivity of the dark-adapted eyecup ERG had a peak at about 520 nm and was well fit by a vitamin A2 nomogram pigment with a lambdamax = 520 nm. This agrees with the eel photopigment measurements of other investigators. This result implies that a single spectral mechanism--the rods--provides the input for the dark-adapted ERG. The spectral sensitivity of the ERG to flicker in the light-adapted eyecup preparation was shifted to longer wavelengths; it peaked at around 550 nm. However, there was evidence that this technique might not have completely eliminated rod intrusion. Rod responses were abolished in a bleached isolated retina preparation, in which it was shown that there were two classes of cone-like mechanisms, one with lambdamax of 550 nm and the other with lambdamax of less than 450 nm. Ganglion cell recording provided preliminary evidence for opponent-color processing. Horizontal cells were only of the L type with both rod and cone inputs.

The cone/horizontal cell network: A possible site for color constancy

1998 ◽  
Vol 15 (5) ◽  
pp. 787-797 ◽  
Author(s):  
M. KAMERMANS ◽  
D.A. KRAAIJ ◽  
H. SPEKREIJSE
Keyword(s):  
Horizontal Cell ◽  
Color Constancy ◽  
Spectral Composition ◽  
Color Discrimination ◽  
Cell System ◽  
Horizontal Cells ◽  
Feedback Signal ◽  
Color Contrast ◽  
Cell Network ◽  
Simultaneous Color Contrast

Color vision is spectrally opponent, suggesting that spectrally opponent neurons, such as the horizontal cells in fish and turtle retinae, play a prominent role in color discrimination. In the accompanying paper (Kraaij et al., 1998), it was shown that the output signal of the horizontal cell system to the cones is not at all spectrally opponent. Therefore, a role for the spectrally opponent horizontal cells in color discrimination seems unlikely. In this paper, we propose that the horizontal cells play a prominent role in color constancy and simultaneous color contrast instead of in color discrimination. We have formulated a model of the cone/horizontal cell network based on measurements of the action spectra of the cones and of the feedback signal of the horizontal cell system to the various cone types. The key feature of the model is (1) that feedback is spectrally and spatially very broad and (2) that the gain of the cone synapse strongly depends on the feedback strength. This makes the synaptic gain of the cones strongly dependent on the spectral composition of the surround. Our model, which incorporates many physiological details of the outer retina, displays a behavior that can be interpreted as color constancy and simultaneous color contrast. We propose that the horizontal cell network modulates the cone synaptic gains such that the ratios of the cone outputs become almost invariant with the spectral composition of the global illumination. Therefore, color constancy appears to be coded in the retina.

The light-induced reduction of horizontal cell receptive field size in the goldfish retina involves nitric oxide

2011 ◽  
Vol 28 (2) ◽  
pp. 137-144 ◽  
Author(s):  
BRYAN A. DANIELS ◽  
WILLIAM H. BALDRIDGE
Keyword(s):  
Nitric Oxide ◽  
Receptive Field ◽  
Horizontal Cell ◽  
Receptive Fields ◽  
Horizontal Cells ◽  
Field Size ◽  
Nitric Oxide Donor ◽  
Nitric Oxide Synthase Inhibitor ◽  
Receptive Field Size ◽  
Goldfish Retina

AbstractHorizontal cells of the vertebrate retina have large receptive fields as a result of extensive gap junction coupling. Increased ambient illumination reduces horizontal cell receptive field size. Using the isolated goldfish retina, we have assessed the contribution of nitric oxide to the light-dependent reduction of horizontal cell receptive field size. Horizontal cell receptive field size was assessed by comparing the responses to centered spot and annulus stimuli and from the responses to translated slit stimuli. A period of steady illumination decreased the receptive field size of horizontal cells, as did treatment with the nitric oxide donor (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate (100μM). Blocking the endogenous production of nitric oxide with the nitric oxide synthase inhibitor, NG-nitro-l-arginine methyl ester (1 mM), decreased the light-induced reduction of horizontal cell receptive field size. These findings suggest that nitric oxide is involved in light-induced reduction of horizontal cell receptive field size.

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