Cryopreservation of zygotic embryos and kernels of oil palm (Elaeis guineensis Jacq.)

AbstractIn the present study, the efficiency of two cryopreservation strategies for oil palm (Elaeis guineensis Jacq.) was compared. If extracted from rehydrated kernels, 65% of the embryos desiccated to around 0.3 g H2O/g DW developed into plantlets after cryopreservation. In contrast, only 25% of embryos (0.12 g H2O/g DW) extracted from cryopreserved dry kernels developed into plantlets. However, this value was increased to 63% if kernels were partially rehydrated before freezing until the water content of embryos reached 0.3 g H2O/g DW. This study emphasizes the importance of partial rehydration of oil palm embryos before cryopreservation.

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New approaches to improve the efficiency of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.) from mature zygotic embryos

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-012-9479-3 ◽

2013 ◽

Vol 49 (1) ◽

pp. 41-50 ◽

Cited By ~ 33

Author(s):

Talita Aparecida Balzon ◽

Zanderluce Gomes Luis ◽

Jonny Everson Scherwinski-Pereira

Keyword(s):

Somatic Embryogenesis ◽

Oil Palm ◽

Elaeis Guineensis ◽

Zygotic Embryos ◽

New Approaches ◽

Elaeis Guineensis Jacq

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Data on germination, growth and morphological changes of oil palm (Elaeis guineensis Jacq.) zygotic embryos during in vitro culturing

Data in Brief ◽

10.1016/j.dib.2019.104975 ◽

2020 ◽

Vol 28 ◽

pp. 104975

Author(s):

D.S. Sparjanbabu ◽

H.S. Prasanna ◽

D. Ramajayam ◽

P. Naveenkumar ◽

M.S.R. Krishna ◽

...

Keyword(s):

Oil Palm ◽

Elaeis Guineensis ◽

Morphological Changes ◽

Zygotic Embryos ◽

Elaeis Guineensis Jacq

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Effect of Plant Gowth Regulator on The Gowth of Zygotic Embryos in Three Types of Oil Palm Fruit (Elaeis guineensis Jacq.) in Tissue Culture

Jurnal Lahan Suboptimal ◽

10.33230/jlso.9.2.2020.474 ◽

2020 ◽

Vol 9 (2) ◽

pp. 149-159

Author(s):

Hadi Surahman ◽

Gogoh Sulaksono ◽

Zulhermana Sembiring ◽

Astuti Kurnianingsih ◽

Dwi Putro Priadi ◽

...

Keyword(s):

Tissue Culture ◽

Embryo Culture ◽

Oil Palm ◽

Elaeis Guineensis ◽

Zygotic Embryos ◽

Palm Fruit ◽

Randomized Design ◽

Elaeis Guineensis Jacq ◽

Positive Results ◽

Germination Stage

Surahman H, Sulaksono G, Sembiring Z, Kurnianingsih A, Priadi DP, Asmono D. 2020. Effect of plant gowth regulator on the gowth of zygotic embryos in three types of oil palm fruit (Elaeis guineensis Jacq.) in tissue culture. Jurnal Lahan Suboptimal : Journal of Suboptimal Lands 9(2): 149-159.Embryo culture is a widely used and effective way of overcoming the dormancy nature of seeds in oil palm. At the germination stage, the influence of gowth regulators play an important role although in relatively lower concentrations in the germination media. This aim research determined the effect of adding various concentrations of gowth regulator on the three types of oil palm fruit based on the thickness of the shell. This research carried out at the Tissue Culture Laboratory of PT. Sampoerna Ago Tbk. from September 2018 to January 2019. The gowth regulator substances used in this study were NAA, BAP and GA3. The composition of the gowth regulators used were H1 = 0.05 mg/L NAA + 0.1 mg/L BAP + 0.1 mg/L GA3, H2 = 0.05 mg/L NAA + 0.3 mg/L BAP + 0.1 mg/L GA3, H3 = 0.05 mg/L NAA + 0.5 mg/L BAP + 0.1 mg/L GA3 and H4 = No gowth regulator. While the types of oil palm fruit used were C1 = Dura, C2 = Tenera and C3 = Pisifera. This research used a factorial complete randomized design with three replications. The results showed that in general the use of gowth regulator substances H1 = 0.05 mg/L NAA + 0.1 mg/L BAP + 0.1 mg/L GA3 and the type of dura fruit showed positive results based on viability, scoring, survival and height of plantlets when compared with other treatments.

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Effect of culture media, plant growth regulators and genotypes on growth and developmental stages of oil palm (Elaeis guineensis Jacq.) zygotic embryos

Indian Journal of Agricultural Research ◽

10.18805/a-5129 ◽

2019 ◽

Author(s):

D.S. Sparjanbabu ◽

P. Naveen Kumar ◽

M.S.R. Krishna ◽

D. Ramajayam ◽

B. Kalyana Babu ◽

...

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Seed Production ◽

Growth Regulators ◽

Oil Palm ◽

Elaeis Guineensis ◽

Culture Media ◽

Zygotic Embryos ◽

Elaeis Guineensis Jacq

Oil palm (Elaeis guineensis Jacq.) has become the largest traded vegetable oil in the world due to its innumerable uses and its huge economic importance. Which increases the requirement of quality seedling but the conventional seed production limits the commercial seed production with its long germination period and low germination rate. In this context in vitro germination of zygotic embryo could be a promising alternative. Hence in this study the effect of culturing media, plant growth regulators and genotypes were assessed on in vitro regeneration and plantlet development of oil palm zygotic embryos. Zygotic embryos of four elite genotypes were cultured on MS, N6 and Y3 media, with or without plant growth regulators (NAA, BA and GA3-0.2mg/lt). There was no effect of culture media, plant growth regulators and genotypes on germination of zygotic embryos. Whereas on the growth and development of in vitro plantlets, there was a significant effect of culture media and genotypes. Where culture media N6 performs well and Y3 has shown more abnormality. In case of genotypes, genotypes G1 and G2 shown better performance when compared to genotypes G3 and G4. The effect of plant growth regulators on germination and plant growth was non significant.

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