scholarly journals Effect of Plant Gowth Regulator on The Gowth of Zygotic Embryos in Three Types of Oil Palm Fruit (Elaeis guineensis Jacq.) in Tissue Culture

2020 ◽  
Vol 9 (2) ◽  
pp. 149-159
Author(s):  
Hadi Surahman ◽  
Gogoh Sulaksono ◽  
Zulhermana Sembiring ◽  
Astuti Kurnianingsih ◽  
Dwi Putro Priadi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Embryo Culture ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Zygotic Embryos ◽  
Palm Fruit ◽  
Randomized Design ◽  
Elaeis Guineensis Jacq ◽  
Positive Results ◽  
Germination Stage

Surahman H, Sulaksono G, Sembiring Z, Kurnianingsih A, Priadi DP, Asmono D. 2020. Effect of plant gowth regulator on the gowth of zygotic embryos in three types of oil palm fruit (Elaeis guineensis Jacq.) in tissue culture. Jurnal Lahan Suboptimal : Journal of Suboptimal Lands 9(2): 149-159.Embryo culture is a widely used and effective way of overcoming the dormancy nature of seeds in oil palm.  At the germination stage, the influence of gowth regulators play an important role although in relatively lower concentrations in the germination media. This aim research determined the effect of adding various concentrations of gowth regulator on the three types of oil palm fruit based on the thickness of the shell. This research carried out at the Tissue Culture Laboratory of PT. Sampoerna Ago Tbk. from September 2018 to January 2019. The gowth regulator substances used in this study were NAA, BAP and GA3. The composition of the gowth regulators used were H1 = 0.05 mg/L NAA + 0.1 mg/L BAP + 0.1 mg/L GA3, H2 = 0.05 mg/L NAA + 0.3 mg/L BAP + 0.1 mg/L GA3, H3 = 0.05 mg/L NAA + 0.5 mg/L BAP + 0.1 mg/L GA3 and H4 = No gowth regulator. While the types of oil palm fruit used were C1 = Dura, C2 = Tenera and C3 = Pisifera. This research used a factorial complete randomized design with three replications. The results showed that in general the use of gowth regulator substances H1 = 0.05 mg/L NAA + 0.1 mg/L BAP + 0.1 mg/L GA3 and the type of dura fruit showed positive results based on viability, scoring, survival and height of plantlets when compared with other treatments.

scholarly journals Morphogenesis of Oil Palm Fruit (Elaeis guineensis Jacq.) in Mesocarp and Endocarp Development

2013 ◽  
Vol 7 (2) ◽  
Author(s):  
Hermine Bille Ngalle ◽  
Joseph Martin Bell ◽  
Georges Franck Ngando Ebongue ◽  
Lambert Nyobe ◽  
Félix Chancelin Ngangnou ◽  
...  
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Palm Fruit ◽  
Elaeis Guineensis Jacq

Cryopreservation of zygotic embryos and kernels of oil palm (Elaeis guineensis Jacq.)

1995 ◽  
Vol 5 (2) ◽  
pp. 81-86 ◽  
Author(s):  
F. Engelmann ◽  
N. Chabrillange ◽  
S. Dussert ◽  
Y. Duval
Keyword(s):  
Water Content ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Zygotic Embryos ◽  
Elaeis Guineensis Jacq

AbstractIn the present study, the efficiency of two cryopreservation strategies for oil palm (Elaeis guineensis Jacq.) was compared. If extracted from rehydrated kernels, 65% of the embryos desiccated to around 0.3 g H2O/g DW developed into plantlets after cryopreservation. In contrast, only 25% of embryos (0.12 g H2O/g DW) extracted from cryopreserved dry kernels developed into plantlets. However, this value was increased to 63% if kernels were partially rehydrated before freezing until the water content of embryos reached 0.3 g H2O/g DW. This study emphasizes the importance of partial rehydration of oil palm embryos before cryopreservation.

scholarly journals Respons bibit kelapa sawit (Elaeis guineensis Jacq.) terhadap pemberian fungi mikoriza arbuskular dan cekaman air [Oil palm (Elaeis guineensis Jacq.) seedling response to application of arbuscular mycorrhiza fungi and water stress]

2017 ◽  
Vol 84 (2) ◽  
Author(s):  
Maria Viva RINI ◽  
Usnaqul EFRIYANI
Keyword(s):  
Water Stress ◽  
Arbuscular Mycorrhiza ◽  
Seedling Growth ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Dry Weight ◽  
Randomized Design ◽  
Arbuscular Mycorrhiza Fungi ◽  
Elaeis Guineensis Jacq ◽  
Glomus Sp

 The objective of this study was to determine the response of oil palm seedling to the application of arbuscular mycorrhiza fungi (AMF) subjected to water stress.  The treatment design used was a factorial design 2 x 5 arranged in completely randomized design.  The first factor was application of AMF i.e. without AMF (control) and inoculation with AMF (mixture of Glomus sp., Entropospora sp., and Gigaspora sp.).  The second factor was water stress i.e. no watering for 0, 7, 14, 21, and 28 days at the end of experiment.  Data obtained were subjected to Bartlett test to examine the treatment variance, and Tukey tests to examine additiveness of the data, and continued by analysis of variance. The results showed that application of AMF increased oil palm seedling growth through the increase in seedling height, number of leave, shoot fresh and dry weight, and percent of root infection.  Duration of water stress significantly affected oil palm seedling growth by decreasing plant height, number of leave, shot and root fresh weight, root dry weight. However, at any level of water stress, the AMF inoculated seedling had better growth compared to those control palms.[Key words: oil palm seedling, arbuscular mycorrhiza fungi (AMF), water stress] AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh pemberian fungi mikoriza arbuskular (FMA) terhadap respons pertumbuhan bibit kelapa sawit yang mengalami cekaman air. Perlakuan disusun secara faktorial 2x5 dalam rancangan kelompok teracak sempurna (RKTS) dengan lima ulangan.  Faktor pertama adalah aplikasi FMA, yaitu kontrol (tanpa FMA) dan diberi FMA (campuran Glomus sp., Entropospora sp., dan Gigaspora sp.).  Faktor kedua adalah lamanya cekaman air, yaitu 0, 7, 14, 21, dan 28 hari tanpa disiram sebelum penelitian dihentikan.  Data yang diperoleh diuji kesamaan ragam antar perlakuannya dengan Uji Bartlett dan kemenambahan modelnya dengan Uji Tukey.  Jika asumsi terpenuhi, ragam homogen dan data bersifat menambah, maka data dianalisis ragam. Hasil penelitian menunjukkan bahwa aplikasi FMA meningkatkan pertumbuhan bibit kelapa sawit melalui peningkatan tinggi tanaman, jumlah daun, bobot basah tajuk, bobot kering tajuk, dan persen infeksi akar. Lamanya cekaman air menurunkan pertumbuhan bibit kelapa sawit melalui penurunan tinggi tanaman, jumlah daun, jumlah akar primer, bobot basah tajuk, bobot basah akar, bobot kering tajuk, dan bobot kering akar, akan tetapi pada setiap  taraf cekaman air, pertumbuhan bibit yang ber-FMA lebih tinggi dibandingkan dengan tanpa FMA.[Kata kunci: bibit kelapa sawit, fungi mikoriza arbuskular (FMA), cekaman air]

scholarly journals UJI PEMBERIAN KOMPOS TANDAN KOSONG SAWIT DAN NPK 16:16:16 PADA PEMBIBITAN KELAPA SAWIT(Elaeis guineensis Jacq.) MAIN NURSERY DENGAN MEDIA SUB SOIL ULTISOL

2019 ◽  
Vol 33 (3) ◽  
pp. 275-284
Author(s):  
Aminullah Aminullah ◽  
T. Rosmawati ◽  
Sulhaswardi Sulhaswardi
Keyword(s):  
Plant Height ◽  
Oil Palm ◽  
Root Length ◽  
Elaeis Guineensis ◽  
Stem Length ◽  
Palm Fruit ◽  
Main Effect ◽  
Two Factors ◽  
Elaeis Guineensis Jacq ◽  
Height Increase

The purpose of this research is to know the influence of compost TKKS and NPK 16:16:16 on the breeding of oil palm (Elaeis guineensis Jacq.) in Main Breeding with Sub Soil Ultisol Media. This research has been conducted in the field of Agriculture Faculty of the Islamic University of Riau. for 4 months, starting from April to August 2017. The design used in this research is Factorial Random Design (RAL) consisting of two factors, the first factor is Giving of empty palm fruit bunch compost (Factor K) consisting of 4 levels ie not giving empty bunches of palm oil (K0), giving compost of oil palm empty bunch 90 gr/plant (K1), 180 gr/plant (K2) and 270 gr/plant (K3), and NPK 16:16:16 Fertilizer (Factor P) consisting of 4 levels ie not giving fertilizer (P0), NPK 16:16:16 15 g/plant (P1), 30 gr/plant (P2), 45 gr/plant (P3) and 60 gr/plant (P4). The parameters observed in this study were the increase of plant height (cm), the increase of the number of stem (strands), the length of the longest stem length (cm), the increase of girth (cm), the number of root root (root) and root length (cm). The result of the research showed that the interaction of TKKS compost and NPK 16:16:16 fertilizer gave a significant effect on the plant height increase, the increase of midrib, the length of the longest bark and the increase of girth. Combination of best treatment on compost TKKS 270 g/plant and NPK 16:16:16 60 g/plant (K3P4) fertilizer. The main effect of TKKS compost and NPK 16:16:16 fertilizer gave a significant effect on all parameters in the range of plant height, the increase of midrib, the length of the longest stem, the increase of girth, the number of root roots and the longest root length. With the best treatment is the compost TKKS 270 g/plant (P3) and fertilizer NPK 16:16:16 60 g/plant (P4).

THE ROLE OF NAA, GA3, ACTIVATED CHARCOAL, AND SUCROSE ON ZYGOTIC EMBRYOS CULTURE OF HYBRID OG OPEN POLLINATED CLONE (Elaeis guineensis JACQ. X Elaeis oleifera)

2018 ◽  
Vol 24 (3) ◽  
pp. 115-126
Author(s):  
Ernayunita Ernayunita ◽  
Hernawan Yuli Rahmadi ◽  
Iman Yani Harahap ◽  
Abdul Razak Purba
Keyword(s):  
Embryo Culture ◽  
Oil Palm ◽  
Activated Charcoal ◽  
Elaeis Guineensis ◽  
Basic Medium ◽  
Zygotic Embryos ◽  
Study Objective ◽  
Hybrid Embryo

Oil palm OG hybrid genetic potentials are high in term of vegetative and generative traits, such as: low height increment, compact palm, high free fatty acid and high carotenes. However, OG hybrid reproduction is hindered due to its low germination rate. So, alternative method for germination is needed, which is in vitro embryo zygotic culture. This study objective was to reveal the best medium composition for OG hybrid embryo culture by modificated several oil palm basic medium from oil palm tissue culture protocol and to understand the role of plant growth regulators, activated charcoal, and sucrose on in vitro growth and development of OG hybrid embryo. The design used was Completely Randomized Design (CRD) with 16 replication and 4 embryos for each replicates, while the treatments were: A1 = M129 (control); A2 = M129 + activated charcoal; A3 = M129+ activated charcoal + sucrose; A4 = ½ M129 + activated charcoal + sucrose; A5 = M129 + GA3 ; and A6 = M129 + GA3 + NAA. From the study we found that the best medium for OG hybrid open pollinated clone's embryo culture was M129 with GA3 and NAA (A6). This medium gave percentage of in vitro germination up to 14.06%, and later on the plantlets from this medium gave the best vegetative growth performance such as high number of leaves and primer roots.

Comparative mineral and hormonal analyses of wild type and TLS somaclonal variant derived from oil palm (Elaeis guineensis Jacq. var. tenera) tissue culture

2012 ◽  
Vol 68 (2) ◽  
pp. 313-317 ◽  
Author(s):  
S. H. Habib ◽  
S.-E. Ooi ◽  
Ondřej Novák ◽  
Danuše Tarkowská ◽  
Jakub Rolčík ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Wild Type ◽  
Somaclonal Variant ◽  
Elaeis Guineensis Jacq
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