Production, carcass and meat quality traits of F2-crosses between European Wild Pigs and domestic pigs including halothane gene carriers

1995 ◽  
Vol 61 (2) ◽  
pp. 325-331 ◽  
Author(s):  
K. Lundström ◽  
A. Karlsson ◽  
J. Håkansson ◽  
I. Hansson ◽  
M. Johansson ◽  
...  

AbstractA pedigree originally designed for pig genome mapping was used in order to study carcass and muscle development as well as meat quality of F2-crosses between the European Wild Pig and the domestic Large White pig (no. = 191). As a marker for the influence of genes inherited from the ‘wild’ and domestic grandparents, grouping based on the length of the small intestine was used. The pigs were also typed for genetic polymorphism at the calcium release channel (CRO locus controlling halothane susceptibility in pigs, and one of the Wild boars was found to be a carrier of the halothane mutation. At assessment, the carcasses were divided into cuts, the back and ham were defatted, and m. longissimus dorsi and the large muscles of the ham were weighed separately.Wlien grouped according to length of the small intestine, those animals with a short small intestine were more similar to Wild Pig, e.g. they were older at slaughter and had a slower growth rate than the other group. Their carcasses contained less lean meat, more flare fat and more subcutaneous fat. No difference in meat quality could be discerned between the groups, except for a tendency to greater pigment content and higher shear force value in the group with a short small intestine.The effect of the halothane mutation at the CRC locus was very prominent, although no animals had the gene in homozygous form. Carriers of the gene were leaner and had a higher reflectance value, lower water-holding capacity, lower pHu, higher protein denaturation and higher shear force value.

2003 ◽  
Vol 46 (5) ◽  
pp. 445-454
Author(s):  
U. Küchenmeister ◽  
G. Kuhn

Abstract. The meat quality of pigs is essentially dependent on the rate and intensity of energy metabolism after slaughter. The major cellular processes in muscle cells are regulated by the Ca2+ concentration in the cytoplasm, stimulating different energy consuming ATPases. The most essential regulator of the Ca2+ concentration is the sarcoplasmic reticulum (SR) with its membranes, the SR Ca2+ ATPase (Ca2+ pump), and the calcium release channel (CRC). Defects of one or more of these elements will be of influence on the metabolism and ultimately on the meat quality. This is widely investigated in pigs with a mutated CRC. However, pigs without a mutation in the CRC also show a wide variability in their meat quality, dependent on other factors e.g. stress or season of the year. The variability in meat quality in these "normal" pigs is at least partly a result of differences in SR Ca2+ transport and the resulting metabolism.


2004 ◽  
Vol 380 (1) ◽  
pp. e1-e3 ◽  
Author(s):  
Tommie V. McCARTHY ◽  
John J. MACKRILL

Ryanodine receptors (RyRs) are a family of intracellular channels that mediate Ca2+ release from the endoplasmic and sarcoplasmic reticulum. More than 50 distinct point mutations in one member of this family, RyR1, cause malignant hyperthermia, a potentially lethal pharmacogenetic disorder of skeletal muscle. These mutations are not randomly distributed throughout the primary structure of RyR1, but are grouped in three discrete clusters. In this issue of the Biochemical Journal, Kobayashi et al. present evidence that interdomain interactions between two of these mutation-enriched regions play a key role in the gating mechanism of RyR1.


2002 ◽  
Vol 278 (1) ◽  
pp. 444-453 ◽  
Author(s):  
Steven Reiken ◽  
Marta Gaburjakova ◽  
Silvia Guatimosim ◽  
Ana M. Gomez ◽  
Jeanine D'Armiento ◽  
...  

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Dorian V. Ziegler ◽  
David Vindrieux ◽  
Delphine Goehrig ◽  
Sara Jaber ◽  
Guillaume Collin ◽  
...  

AbstractCellular senescence is induced by stresses and results in a stable proliferation arrest accompanied by a pro-inflammatory secretome. Senescent cells accumulate during aging, promoting various age-related pathologies and limiting lifespan. The endoplasmic reticulum (ER) inositol 1,4,5-trisphosphate receptor, type 2 (ITPR2) calcium-release channel and calcium fluxes from the ER to the mitochondria are drivers of senescence in human cells. Here we show that Itpr2 knockout (KO) mice display improved aging such as increased lifespan, a better response to metabolic stress, less immunosenescence, as well as less liver steatosis and fibrosis. Cellular senescence, which is known to promote these alterations, is decreased in Itpr2 KO mice and Itpr2 KO embryo-derived cells. Interestingly, ablation of ITPR2 in vivo and in vitro decreases the number of contacts between the mitochondria and the ER and their forced contacts induce premature senescence. These findings shed light on the role of contacts and facilitated exchanges between the ER and the mitochondria through ITPR2 in regulating senescence and aging.


Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 215 ◽  
Author(s):  
Jacob R. Tuell ◽  
Jun-Young Park ◽  
Weichao Wang ◽  
Bruce Cooper ◽  
Tiago Sobreira ◽  
...  

The objective of this study was to evaluate the effects of photoperiod on meat quality, oxidative stability, and metabolites of broiler fillet (M. Pectoralis major) muscles. A total of 432 broilers was split among 4 photoperiod treatments [hours light(L):dark(D)]: 20L:4D, 18L:6D, 16L:8D, and 12L:12D. At 42 days, a total of 48 broilers (12 broilers/treatment) was randomly selected and harvested. At 1 day postmortem, fillet muscles were dissected and displayed for 7 days. No considerable impacts of photoperiods on general carcass and meat quality attributes, such as carcass weight, yield, pH, water-holding capacity, and shear force, were found (p > 0.05). However, color and oxidative stability were influenced by the photoperiod, where muscles from 20L:4D appeared lighter and more discolored, coupled with higher lipid oxidation (p < 0.05) and protein denaturation (p = 0.058) compared to 12L:12D. The UPLC–MS metabolomics identified that 20 metabolites were different between the 20L:4D and 12L:12D groups, and 15 were tentatively identified. In general, lower aromatic amino acids/dipeptides, and higher oxidized glutathione and guanine/methylated guanosine were observed in 20L:4D. These results suggest that a photoperiod would result in no considerable impact on initial meat quality, but extended photoperiods might negatively impact oxidative stability through an alteration of the muscle metabolites.


2009 ◽  
Vol 96 (3) ◽  
pp. 96a ◽  
Author(s):  
Que T. Ngo ◽  
Joshua T. Maxwell ◽  
Gregory A. Mignery ◽  
Wah Chiu ◽  
Steven J. Ludtke ◽  
...  

1996 ◽  
Vol 71 (6) ◽  
pp. 2996-3012 ◽  
Author(s):  
A. Zahradníková ◽  
I. Zahradník

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