scholarly journals Effects of Photoperiod Regime on Meat Quality, Oxidative Stability, and Metabolites of Postmortem Broiler Fillet (M. Pectoralis major) Muscles

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 215 ◽  
Author(s):  
Jacob R. Tuell ◽  
Jun-Young Park ◽  
Weichao Wang ◽  
Bruce Cooper ◽  
Tiago Sobreira ◽  
...  

The objective of this study was to evaluate the effects of photoperiod on meat quality, oxidative stability, and metabolites of broiler fillet (M. Pectoralis major) muscles. A total of 432 broilers was split among 4 photoperiod treatments [hours light(L):dark(D)]: 20L:4D, 18L:6D, 16L:8D, and 12L:12D. At 42 days, a total of 48 broilers (12 broilers/treatment) was randomly selected and harvested. At 1 day postmortem, fillet muscles were dissected and displayed for 7 days. No considerable impacts of photoperiods on general carcass and meat quality attributes, such as carcass weight, yield, pH, water-holding capacity, and shear force, were found (p > 0.05). However, color and oxidative stability were influenced by the photoperiod, where muscles from 20L:4D appeared lighter and more discolored, coupled with higher lipid oxidation (p < 0.05) and protein denaturation (p = 0.058) compared to 12L:12D. The UPLC–MS metabolomics identified that 20 metabolites were different between the 20L:4D and 12L:12D groups, and 15 were tentatively identified. In general, lower aromatic amino acids/dipeptides, and higher oxidized glutathione and guanine/methylated guanosine were observed in 20L:4D. These results suggest that a photoperiod would result in no considerable impact on initial meat quality, but extended photoperiods might negatively impact oxidative stability through an alteration of the muscle metabolites.

2017 ◽  
Vol 62 (No. 2) ◽  
pp. 51-57 ◽  
Author(s):  
A. Dalle Zotte ◽  
G. Tasoniero ◽  
E. Puolanne ◽  
H. Remignon ◽  
M. Cecchinato ◽  
...  

The purposes of the study were to investigate the effects of Wooden Breast (WB) myodegeneration on poultry meat quality and to give a contribution in typing lesions morphology. At a poultry meat cutting facility, 474 carcasses of a high-breast-yield hybrid chickens were inspected for WB condition, and 30 normal (N) and 30 affected (WB) breast fillets (Pectoralis major) were randomly selected. The WB condition represented 53.2% of the examined carcasses. Weight, cross sectional area (CSA), pH, L*, a*, b* colour values, water-holding capacity, and Warner-Bratzler shear force were determined. Samples were also visually and histologically evaluated. Affected samples were heavier, thicker, paler (P &lt; 0.001), and characterized by palpatory hardness and lower water holding capacity (P &lt; 0.05). Macroscopically, abnormalities were primarily localized in the cranial portion of the fillet and defined by the presence of bulges, petechiae, fluid and clear exudate, and higher pH. Microscopically, the WB condition was characterized by muscle fibres with greater CSA (P &lt; 0.001) and higher giant fibres prevalence (P &lt; 0.01). Data suggest a relationship between breast weight and WB condition.


2020 ◽  
Vol 60 (5) ◽  
pp. 713 ◽  
Author(s):  
A. Maggiolino ◽  
J. M. Lorenzo ◽  
A. Salzano ◽  
M. Faccia ◽  
F. Blando ◽  
...  

Context The inclusion of Pinus taeda hydrolysed lignin (PTHL) in beef diets could improve quality and stability of meat, but effects could vary through the aging period (AP). Aim The aim was to evaluate the effects of the PTHL inclusion in the diet of finishing beef cattle on meat quality, fatty acid composition and oxidative stability at Days 1, 8, 11 and 15 of aging. Methods Forty Limousin bulls (340 ± 42 kg) were fed ad libitum on a total mixed ration (TMR). The control group received exclusively TMR for 120 days, while the experimental group received the same TMR as the control group but supplemented with PTHL (Oxifenol, I-Green, Padua, Italy; 35 g/day per head at 1–90 days and 70 g/day per head at 91–120 days). Key results Diet did not influence the chemical composition, pH, cooking loss, Warner–Bratzler shear force and hydroperoxide content. The Warner–Bratzler shear force (P &lt; 0.001) decreased, while lightness (P &lt; 0.01) and hydroperoxides (P &lt; 0.001) increased through the aging period. Thiobarbituric acid reactive substances were similar for both diets at 1 and 15 days. However, thiobarbituric acid reactive substances at 8 and 11 days were higher for control than for the PTHL diet (P = 0.023 for interaction). Protein carbonyls were higher for control than for the PTHL diet at 8 days (P = 0.003 for interaction), but similar for both diets for the other dates. Saturated, monounsaturated and polyunsaturated fatty acids varied through the AP with PTHL diet, while no changes were observed with control diet (P &lt; 0.01 for interactions). At 11 days, the n-6:n-3 ratio passed from being the minimum value with the PTHL diet to be the maximum with control diet (P &lt; 0.01 for interaction). Conclusions The effects of PTHL inclusion in bull finishing diets depends on the AP but, generally, may result in beef with meat with beneficial effects on human health. Implications Including PTHL in the diet of finishing bulls can be useful to improve meat quality, favouring the use of natural waste substances deriving from vegetal production.


2016 ◽  
Vol 96 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Seul Gi Kwon ◽  
Jung Hye Hwang ◽  
Da Hye Park ◽  
Tae Wan Kim ◽  
Deok Gyeong Kang ◽  
...  

Corticosteroid-binding globulin (CBG) is a plasma transport protein that has glucocorticoid-binding activity. In the present study, we identified CBG gene expression in several tissues of four pig breeds: Berkshire, Duroc, Landrace, and Yorkshire. Expression of CBG mRNA was detected in the liver of all four breeds, and was the highest in Berkshire pigs. We also found single nucleotide polymorphisms (SNPs) in the CBG gene from Berkshire pigs, including SNP c.919G>A, which corresponds to 307G>R. We analyzed the relationships between this CBG variant and various meat-quality traits. The SNP was significantly associated with backfat thickness, post-mortem pH24h, meat color [CIE a* (redness), CIE b* (yellowness)], water-holding capacity, fat content, moisture content, protein content, cooking loss, and shear force. However, the effects differed by gender: the values were significantly associated with almost all traits in gilts, whereas only cooking loss and shear force were shown significantly in barrows. The variant G allele was associated with decreases in backfat thickness, post-mortem pH24h, CIE a*, fat content, and cooking loss, but with increases in CIE b*, water-holding capacity, moisture content, protein content, and shear force. Because the general correlation between meat-quality traits were not validated in the present study, we suggest that certain SNP might be used in the restrictive application to distinguish meat-quality traits.


1995 ◽  
Vol 61 (2) ◽  
pp. 325-331 ◽  
Author(s):  
K. Lundström ◽  
A. Karlsson ◽  
J. Håkansson ◽  
I. Hansson ◽  
M. Johansson ◽  
...  

AbstractA pedigree originally designed for pig genome mapping was used in order to study carcass and muscle development as well as meat quality of F2-crosses between the European Wild Pig and the domestic Large White pig (no. = 191). As a marker for the influence of genes inherited from the ‘wild’ and domestic grandparents, grouping based on the length of the small intestine was used. The pigs were also typed for genetic polymorphism at the calcium release channel (CRO locus controlling halothane susceptibility in pigs, and one of the Wild boars was found to be a carrier of the halothane mutation. At assessment, the carcasses were divided into cuts, the back and ham were defatted, and m. longissimus dorsi and the large muscles of the ham were weighed separately.Wlien grouped according to length of the small intestine, those animals with a short small intestine were more similar to Wild Pig, e.g. they were older at slaughter and had a slower growth rate than the other group. Their carcasses contained less lean meat, more flare fat and more subcutaneous fat. No difference in meat quality could be discerned between the groups, except for a tendency to greater pigment content and higher shear force value in the group with a short small intestine.The effect of the halothane mutation at the CRC locus was very prominent, although no animals had the gene in homozygous form. Carriers of the gene were leaner and had a higher reflectance value, lower water-holding capacity, lower pHu, higher protein denaturation and higher shear force value.


Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2438
Author(s):  
Eugeniusz R. Grela ◽  
Małgorzata Świątkiewicz ◽  
Mariusz Florek ◽  
Maciej Bąkowski ◽  
Grzegorz Skiba

In this experiment, we investigated the effect of the supplementation of probiotic bacteria in the diet with inulin or dried Jerusalem artichoke tubers on the performance, meat quality, and fatty acid composition in the meat and backfat of fatteners. One hundred and forty-four crossbred pigs (PIC × Penarlan P76) were divided into six groups and fattened from 30 to 114 kg. The meat proximate composition, pH, color, texture, shear force, water-holding capacity, sensory attributes, and thiobarbituric-acid-reactive substances were measured. Normal post-mortem meat glycolysis was demonstrated and no meat defects were present. The chemical constituents in muscle tissues were similar, except for intramuscular fat (IMF). The addition of the prebiotics resulted in a higher IMF level, whereas a significantly lower content was found after the probiotic supplementation. Meat from both prebiotic groups was lighter, less red, and more yellow and showed a higher hue angle. The addition of both prebiotics significantly improved the antioxidant status of meat (by approximately 16% and 18%) and the water-holding capacity (less free water and higher M/T ratios), but reduced shear force (by 17%, p ≤ 0.05) and hardness (by 39% and 35%, respectively, p ≤ 0.05). The addition of the prebiotics and probiotics had no effect on any of the evaluated sensory attributes.


2015 ◽  
Vol 44 (5) ◽  
pp. 58-63
Author(s):  
B Agbeniga ◽  
EC Webb

In a previous study regarding the effects of Kosher and conventional slaughter techniques on carcass and meat quality of cattle, it was speculated that electrical stimulation may have affected some of the meat qualities. Therefore, the objective of this study was to investigate the effects of electrical stimulation (ES) and non-electrical stimulation (NES) on key carcass and meat quality attributes of cattle slaughtered by Kosher vs. conventional slaughter methods. Carcass pH and temperature profiles over a 24 h post mortem (pm) period, meat shear force and water holding capacity were investigated in feedlot type cattle of comparable weights and breed types. Results showed that the combined effects of slaughter methods did not influence the meat quality attributes, but there were differences within the slaughter groups. The effect of ES on carcass pH lasted longer within the conventionally slaughtered group (12 h), than in the Kosher slaughter group (6 h). Muscle samples from the ES groups for both slaughter methods were more tender. Electrical stimulation also had a significant effect on the cooking loss from Kosher meat, while there was no significant difference in meat from the conventional slaughter methods. The results show that ES influences certain meat and carcass quality attributes of cattle, based on the way cattle were slaughtered.Keywords: Carcass pH, cooking loss, drip loss, electrical stimulation, shear force, slaughter technique


2014 ◽  
Vol 54 (4) ◽  
pp. 414 ◽  
Author(s):  
Y. H. B. Kim ◽  
M. Kerr ◽  
G. Geesink ◽  
R. D. Warner

This study evaluated effects of high pre-rigor temperature and duration and suspension of lamb sides on quality traits and protein denaturation in two muscles [semimembranosus (SM) and longissimus thoracis et lumborum (LTL)]. Twenty-four lamb carcasses, within each of 3 slaughter days, were used to assign eight carcasses to one of four pre-rigor temperature treatments: chilled at 2°C directly after slaughter, or held at 37°C in water for 1.5, 3.0 or 4.5 h before transfer to a 2°C chiller. At ~15 min post slaughter, one side of each carcass was suspended from the Achilles tendon, whereas the other side was suspended by the aitch bone and the leg tied down to the ribs. The sides subjected to aitch bone hanging had an increased sarcomere length in the SM, but decreased sarcomere length in the LTL. For the LTL, the time of exposure to high pre-rigor temperature had a significant effect on measures of protein denaturation and related meat quality traits such as purge and colour, although tenderness (shear force) after 2 days of aging was not affected. For the SM, the high temperature treatment also resulted in increase in measures of protein denaturation and thus negatively influenced meat quality traits such as purge, colour and shear force after aging. However, these effects on purge and shear force in the SM were significantly mitigated by the aitchbone hanging treatment. The results of the present experiment indicate that pre-rigor aitchbone hanging of muscles can counteract the negative effects of high pre-rigor temperature on both water loss and meat tenderness.


Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1311
Author(s):  
María Moreno Díaz ◽  
Valeriano Domenech García ◽  
Carmen Avilés Ramírez ◽  
Francisco Peña Blanco ◽  
Francisco Requena Domenech ◽  
...  

Finishing diets in intensive beef production systems are mainly based on cereals, which does not take advantage of the capacity of the ruminant digestive system to digest fibrous feeds, cannot be considered sustainable and does not contribute to the circular bioeconomy. Our aim was to investigate the effects of an alternative concentrate rich in agro-industrial by-products for finishing crossbred Limousine heifers. Four pens with 12 heifers and four pens with 13 heifers were randomly allocated to one of two treatments: control (CON), a commercial concentrate with a 43.3% cereal composition, and alternative (ALT), a concentrate with a composition of 22% cereals and 73.5% agro-industrial by-products. Growth performance data were collected along the 91 days of the experimental period. Carcass characteristics were collected after slaughter and 24 h later. Vacuum-packaged samples from longissimus muscle were aged for 7, 21 or 28 days to study meat quality traits. Feed intake was higher and feed conversion rate was poorer in the ALT treatment, but no differences were found in average daily gain and feeding costs. Treatment had no effects on any of the measured carcass traits (grading, hot and cold carcass weight, dressing out, chilling losses, subcutaneous fat depth, pH, temperature and lean and fat colour) nor on the meat quality traits (drip loss, cooking loss, shear force, oxidative stability, chromatic indices and pigment contents). Ageing time decreased drip loss and shear force, increased lightness and did not affect redness or surface colour stability. In conclusion, feeding crossbred Limousine heifers a finishing diet rich in agro-industrial by-products did not have any negative effects on performance, carcass and meat quality traits, which might be considered positive from the point of view of sustainability of beef production. Under the conditions assayed, ageing for 21 and 28 days improved tenderness of meat, without detrimental effects on oxidative stability or traits related to visual acceptability.


2001 ◽  
Vol 44 (5) ◽  
pp. 473-488 ◽  
Author(s):  
A. Chambaz ◽  
M. Kreuzer ◽  
M. R. L. Scheeder ◽  
P.-A. Dufey

Abstract. Meat quality of Angus (AN), Simmental (SI), Charolais (CH), Limousin (LI), Blonde d'Aquitaine (BL), and Piedmontese (PI) steers (n = 22 per breed group) was measured in the M. longissimus dorsi (M.l.d.) and the M. biceps femoris, regio glutea (M.b.f.). Animals were fattened in two subsequent series on a forage-based diet until a target level of 3.5 % intramuscular fat (IMF) was reached aecording to real-time ultrasound assessments in the live animals or until 15 months of fattening had passed. Series 1 was performed in a tie-stall barn while a loosehousing system with straw bedding was applied in series 2. The actually measured IMF contents in M.l.d. were 3.35, 3.47, 3.49, 3.48, 2.34 and 2.40 % for AN, SI, CH, LI, BL and PI, respectively. Breed group differences in IMF content were mostly accompanied by a contrary Variation either in muscle water or protein content. Muscle cholesterol levels were similar for all breeds amounting to 47 and 51 mg/100 g on average in M.l.d. and M.b.f, respectively. Early and late postmortem muscle pH was relatively similar among breeds, but water-holding capacity, measured as losses due to drip, ageing, thawing and cooking, was unfavourably high in AN (drip loss excepted) in both muscles. Cooking loss tended to be lowest in PI, drip loss in SI. The AN showed the palest meat. In line with lightness, heme iron contents were clearly lowest in both muscles in the AN steers. There was no relationship found between IMF and shear force among breed groups. No significant differences between breed groups occurred in M.l.d. collagen solubility and shear force. Apart from breed differences, there were several differences noted between fattening series, namely clearly better water-holding capacity and lower shear force of the meat from series 2 (group housing) than from series 1 (tied System). The results indicate that in steers of similar IMF content and raised under the same feeding and management conditions, differences in most M.l.d. and M.b.f. quality traits were apparent, with the exception of shear force and M.l.d. collagen solubility.


2020 ◽  
Vol 33 (10) ◽  
pp. 1642-1655
Author(s):  
Prabhu Balan ◽  
Mustafa M. Farouk ◽  
Maryann Staincliffe ◽  
Adam D Stuart ◽  
Robert Kemp ◽  
...  

Objective: The aim of this study is to characterize the impact of additional electrical stimulation (AES) and various pre-rigor holding temperatures (for 3 h) on the ageing-potential of hot boned bovine M. longissimus lumborum (LL).Methods: Paired LL loins from 12 bulls were hot-boned within 40 min of slaughter, immediate AES applied and subjected to various holding temperatures (5°C, 15°C, 25°C, and 35°C) for 3 h.Results: AES did not accelerate the rate of rigor attainment, but the 3 h pre-rigor holding temperature did. Shear force values decreased as the pre-rigor holding temperatures increased. AES and holding for 3 h (at 25°C) resulted in higher water-holding capacity.Conclusion: Data confirmed that AES did not influence the various meat quality parameters in the present study, but pre-rigor holding temperature (25°C) alone or in combination with AES resulted in superior meat quality.


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