Ultrastructural Changes Due to Toxaphene Insecticide

Author(s):  
T. Caceci ◽  
G. B. Chapman

The deleterious effects of agricultural insecticides on non-target organisms have been under investigation for a number of years. Most studies have dealt with the degree of toxicity, mechanism of action, metabolism, or distribution in the biosphere. There has been comparatively little interest in morphological effects of an insecticide at its site of entry. Toxaphene, a general-purpose organochlorine insecticide that is widely and heavily applied in the U.S. is quite toxic to fish. It can be absorbed via the gills or the gut. This study examined the effects of Toxaphene on the absorptive cells of the anterior intestinal bulb in goldfish (Carassius auratus). These cells are involved in the uptake of dietary lipids; since Toxaphene is lipid-soluble, this was considered the likeliest region of the digestive tract to exhibit damage due to its presence.

Author(s):  
Waykin Nopanitaya ◽  
Joe W. Grisham ◽  
Johnny L. Carson

An interesting feature of the goldfish liver is the morphology of the hepatic plate, which is always formed by a two-cell layer of hepatocytes. Hepatic plates of the goldfish liver contain an infrequently seen second type of cell, in the centers of plates between two hepatocytes. A TEH study by Yamamoto (1) demonstrated ultrastructural differences between hepatocytes and centrally located cells in hepatic plates; the latter were classified as ductule cells of the biliary system. None of the previous studies clearly showed a three-dimensional organization of the two cell types described. In the present investigation we utilize SEM to elucidate the arrangement of hepatocytes and bile ductular cells in intralobular plates of goldfish liver.Livers from young goldfish (Carassius auratus), about 6-10 cm, fed commercial fish food were used for this study. Hepatic samples were fixed in 4% buffered paraformaldehyde, cut into pieces, fractured, osmicated, CPD, mounted Au-Pd coated, and viewed by SEM at 17-20 kV. Our observations were confined to the ultrastructure of biliary passages within intralobular plates, ductule cells, and hepatocytes.


2001 ◽  
Vol 36 (3-4) ◽  
pp. 195-198 ◽  
Author(s):  
I Paschos ◽  
L Natsis ◽  
C Nathanailides ◽  
I Kagalou ◽  
E Kolettas

2021 ◽  
Vol 66 ◽  
pp. 126758
Author(s):  
Javad Seyedi ◽  
Mohammad Reza Kalbassi ◽  
Milad Esmaeilbeigi ◽  
Mohammad Behzadi Tayemeh ◽  
Jamshid Amiri Moghadam

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