Polarization Modulation Differential Interference Contrast (Pol Mod Dic) Microscopy: An Improvement for Video Microscopy

1998 ◽  
Vol 4 (S2) ◽  
pp. 130-131
Author(s):  
N. Stromgren Allen ◽  
D. Moxley ◽  
D. Collings ◽  
G. Holzwarth
Keyword(s):  
Image Processing ◽  
Incident Light ◽  
Plant Cells ◽  
Massively Parallel ◽  
Polarization Modulation ◽  
Ferroelectric Liquid Crystal ◽  
Differential Interference Contrast ◽  
Synchronous Detection ◽  
High Resolution Microscopy ◽  
Dic Microscopy

Differential interference contrast (DIC) light microscopy, particularly when coupled with digital image processing, is a powerful tool for the high-resolution microscopy of unstained, transparent biological specimens and can equally well be applied to semiconductor measurements. We show analytically, and with images of diatoms, plant cells and protoplasts, that switching the polarization of the incident light by 90 degrees, changes the image highlights found in conventional DIC images into shadows and vice versa (1). Using a ferroelectric liquid-crystal modulator, this switching can be done at frame rates, synchronized to the camera. By subtracting alternate frames, a stream of difference DIC images is created. We call this technique Pol Mod DIC. Subtraction of alternate images is carried out efficiently by frame buffer operations and amounts to massively parallel synchronous detection. A similar method has been applied to confocal microscopy (2).

Morphological Characterization of Wax and Surfactant–Encapsulated Microcrystalline Cellulose for Better Dispersion

2020 ◽  
Vol 70 (2) ◽  
pp. 226-231
Author(s):  
Qingzheng Cheng ◽  
Chengfeng Zhou ◽  
Yuanfeng Pan ◽  
Brian Via
Keyword(s):  
Microcrystalline Cellulose ◽  
Morphological Characterization ◽  
Fiber Bundles ◽  
Wood Composite ◽  
Differential Interference Contrast ◽  
Expensive Equipment ◽  
Fine Tune ◽  
Cellulose Composite ◽  
Dic Microscopy

Abstract Encapsulation of cellulose with wax and surfactant is a physical way to restrict cellulose-to-cellulose attraction. Because wax is often used in the wood composite process, industrial manufacturers would not have to upgrade or add expensive equipment to handle cellulose addition. The encapsulated cellulose particles could easily be transported to composite and polymer facilities and blended in a homogeneous fashion for a multitude of products and composites. It was the objective of this study to utilize differential interference contrast (DIC) microscopy to characterize the wax and surfactant coverage and encapsulation morphology of the wax–surfactant–cellulose composite. The lengths and widths of the cellulose particles were significantly changed after encapsulation. DIC microscopy found that we could fine-tune wax coverage to control homogeneity and reduce fiber bundling during dispersion. It was found that surfactants were not necessary to enhance coverage if a 1:4 ratio of wax to microcrystalline cellulose was used. However, if more wax is desired, then surfactants may be necessary to suppress fiber bundles during dispersion.

A Novel, Label-Free Method for Quantifying Collagen Fibril Formation and Degradation Using DIC Microscopy, Electromagnetic Wave Theory, and Image Processing

2009 ◽  
Author(s):  
Brendan P. Flynn ◽  
Amit Bhole ◽  
Charles DiMarzio ◽  
Jeffrey W. Ruberti
Keyword(s):  
Electron Microscopy ◽  
Image Processing ◽  
Electromagnetic Wave ◽  
Collagen Fibril ◽  
Reaction Rate ◽  
Wave Theory ◽  
Label Free ◽  
Averaging Methods ◽  
Rate Kinetics ◽  
Dic Microscopy

Methods to assay fibrillar growth and degradation at sub-light scales include: fluorescence assays using FITC-collagen or FRAP, destructive preparation and measurement using electron microscopy, and light occlusion methods including turbidity and absorption methods. Many of these methods require the outright destruction, or at least modification via labelling, of the sample in question. This requirement can slow experimentation and introduce additional variability or even alter the reaction rate kinetics. The two methods (absorption and turbidity) which are label-free are bulk averaging methods and cannot isolate subsets of fibrils (e.g. fibrils under load).

scholarly journals High-throughput in-focus differential interference contrast imaging of three-dimensional orientations of single gold nanorods coated with a mesoporous silica shell

RSC Advances ◽  
2020 ◽  
Vol 10 (50) ◽  
pp. 29868-29872
Author(s):  
Geun Wan Kim ◽  
Seokyoung Yoon ◽  
Jung Heon Lee ◽  
Ji Won Ha
Keyword(s):  
Mesoporous Silica ◽  
High Throughput ◽  
Gold Nanorods ◽  
Focal Plane ◽  
Three Dimensional ◽  
Silica Shell ◽  
Differential Interference Contrast ◽  
Contrast Imaging ◽  
3D Space ◽  
Dic Microscopy

Spherical AuNRs@mSiO2 have randomly oriented AuNR cores in 3D space, which could be resolved on the same focal plane by interference-based DIC microscopy.

A Scalable Massively Parallel Processor for Real-Time Image Processing

2011 ◽  
Vol 46 (10) ◽  
pp. 2363-2373 ◽  
Author(s):  
Takashi Kurafuji ◽  
Masaru Haraguchi ◽  
Masami Nakajima ◽  
Tetsu Nishijima ◽  
Tetsushi Tanizaki ◽  
...  
Keyword(s):  
Image Processing ◽  
Real Time ◽  
Massively Parallel ◽  
Parallel Processor ◽  
Real Time Image Processing ◽  
Real Time Image ◽  
Massively Parallel Processor ◽  
Time Image
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