Development of Monovalent Fluorescent Probes for Single Particle Tracking of ErbB Receptors

2008 ◽  
Vol 14 (S2) ◽  
pp. 1460-1461
Author(s):  
ST Low-Nam ◽  
BS Wilson ◽  
DS Lidke

Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008

2012 ◽  
Vol 102 (3) ◽  
pp. 31a ◽  
Author(s):  
Meike Heidbreder ◽  
Christin Zander ◽  
Sebastian Malkusch ◽  
Darius Widera ◽  
Deepak Nair ◽  
...  

Entropy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. 498
Author(s):  
Chen Zhang ◽  
Kevin Welsher

In this work, we present a 3D single-particle tracking system that can apply tailored sampling patterns to selectively extract photons that yield the most information for particle localization. We demonstrate that off-center sampling at locations predicted by Fisher information utilizes photons most efficiently. When performing localization in a single dimension, optimized off-center sampling patterns gave doubled precision compared to uniform sampling. A ~20% increase in precision compared to uniform sampling can be achieved when a similar off-center pattern is used in 3D localization. Here, we systematically investigated the photon efficiency of different emission patterns in a diffraction-limited system and achieved higher precision than uniform sampling. The ability to maximize information from the limited number of photons demonstrated here is critical for particle tracking applications in biological samples, where photons may be limited.


Soft Matter ◽  
2021 ◽  
Author(s):  
Katie A. Rose ◽  
Daeyeon Lee ◽  
Russell J. Composto

The effect of static silica particles on the dynamics of quantum dot (QD) nanoparticles grafted with a poly(ethylene glycol) (PEG) brush in hydrogel nanocomposites is investigated using single particle tracking (SPT).


2013 ◽  
Vol 102 (17) ◽  
pp. 173702 ◽  
Author(s):  
Manuel F. Juette ◽  
Felix E. Rivera-Molina ◽  
Derek K. Toomre ◽  
Joerg Bewersdorf

2014 ◽  
Vol 16 (8) ◽  
pp. 085014 ◽  
Author(s):  
Alona Birjiniuk ◽  
Nicole Billings ◽  
Elizabeth Nance ◽  
Justin Hanes ◽  
Katharina Ribbeck ◽  
...  

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