scholarly journals The Commercial Development of FT-IR Microspectroscopy

1993 ◽  
Vol 1 (3) ◽  
pp. 6-7
Author(s):  
John A. Reffner
Keyword(s):  
Fourier Transform ◽  
World War Ii ◽  
World War ◽  
Commercial Development ◽  
10Th Anniversary ◽  
Infrared Microspectroscopy ◽  
Fourier Transform Infrared Microspectroscopy ◽  
Ft Ir ◽  
Ir Microspectroscopy ◽  
Ft Ir Spectroscopy

Pittcon ‘93 marked the 10th anniversary of the introduction of a microscope attachment designed specifically for Fourier transform infrared microspectroscopy. The first commercial microscope developed for FT-IR spectroscopy was designed by Spectra-Tech, Inc., under the direction of then-owner D. W. Sting, in fulfillment of a contract with C. T. Foskett of the Digilab Division of BioRad. Digilab recognized a growing interest in the market for an FT-IR microscope and contracted with Spectra-Tech to design and build a microscope accessory for Digilab's FT-IR spectrometers. This microscope was introduced at the 1983 Pittsburgh Conference.Linking microscopy with Fourier transform spectroscopy was a very significant event, but the foundation of infrared microspectroscopy can be traced back to 1949. The explosive growth in the use of infrared-absorption spectroscopy following World War II led researchers R. Gore (in the U.S.A.), and R. Barer, A. R. Cole, and H. W. Thompson (in England), to investigate (in 1949) the possibility of recording infrared spectra of microscopic samples.

scholarly journals Different Approaches to FT-IR Microspectroscopy on X-ray Exposed Human Cells

Proceedings ◽  
2019 ◽  
Vol 42 (1) ◽  
pp. 18
Author(s):  
Marianna Portaccio ◽  
Federico Manganello ◽  
Roberta Meschini ◽  
Ines Delfino ◽  
Valerio Ricciardi ◽  
...  
Keyword(s):  
Neuroblastoma Cells ◽  
Cell Interaction ◽  
Attenuated Total Reflection ◽  
X Ray ◽  
Infrared Microspectroscopy ◽  
Fourier Transform Infrared Microspectroscopy ◽  
Experimental Approaches ◽  
Ft Ir ◽  
Ir Microspectroscopy

Fourier-Transform Infrared microspectroscopy (μFT-IR) has been usefully applied in the analysis of the complex biological processes occurring during X-ray radiation-cell interaction. Different experimental approaches are available for FT-IR spectra collection (transmission, attenuated total reflection (ATR), and transflection modes) from cells samples. Recently, some problems have been raised about the role of transmitted and reflected components of the infrared beam in transflection mode. For this reason, we investigated two different transflection approaches for collecting spectra from cells exposed to X-ray. In the former approach, cells were grown on MirrIR slides, and for the second approach, cell pellets were prepared. In both cases, SH-SY5Y neuroblastoma cells were used. X-ray exposure was performed at doses of 2 and 4 Gy. Spectra were obtained by using both the approaches in the 600–4000 cm−1 spectral range from exposed and not-exposed samples. The main contributions from proteins, lipids, carbohydrates, and DNA were clearly evidenced in spectra obtained with the two different acquisition approaches. A comparison among them has been also reported.

scholarly journals Fourier-Transform Infrared Microspectroscopy (FT-IR) Study on Caput and Cauda Mouse Spermatozoa

Proceedings ◽  
2019 ◽  
Vol 42 (1) ◽  
pp. 19 ◽  
Author(s):  
Marianna Portaccio ◽  
Sonia Errico ◽  
Teresa Chioccarelli ◽  
Gilda Cobellis ◽  
Maria Lepore
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Biochemical Changes ◽  
Infrared Microspectroscopy ◽  
Fourier Transform Infrared Microspectroscopy ◽  
In Vitro Investigation ◽  
Ft Ir ◽  
Protein Nucleic Acid ◽  
Ir Study

Fourier-Transform Infrared micro-spectroscopy (µFT-IR) was used for an in vitro investigation on spermatozoa (SPZ) samples separately collected from caput and cauda of mouse epididymis. SPZ are characterized by deep biochemical changes during the transit along the epididymis and they can constitute ideal candidates for a µFT-IR investigation, thanks to the ability of this technique in analyzing cells at a molecular level. Appreciable differences were reported in the infrared spectra from caput and cauda SPZ, and biochemical changes in protein, nucleic acid, lipid, and carbohydrate content of cells were evidenced. The present investigation indicates that µFT-IR can constitute a valuable tool for monitoring, in an easy and fast way, the changes suffered by SPZ during the transit along the epididymis.

Fourier Transform Infrared Microspectroscopy of Plant Tissues

1996 ◽  
Vol 50 (3) ◽  
pp. 357-365 ◽  
Author(s):  
Derek Stewart
Keyword(s):  
Cell Wall ◽  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Erwinia Carotovora ◽  
Wall Structure ◽  
Initial Development ◽  
Infrared Microspectroscopy ◽  
Fourier Transform Infrared Microspectroscopy ◽  
Ft Ir ◽  
Lignin Deposition

Fourier transform infrared (FT-IR) microspectroscopy was applied to three distinct types of plant tissue. Reflectance microspectroscopy of nutshells highlighted the differences between the chemistries of the inner and outer surfaces and the tissue as a whole. The outer surfaces were suberized, while the inner surfaces contained absorbances indicative of lignin or tannins or both. Transmission microspectroscopy was used to follow the changes in cell wall structure and composition of flax epidermal cells during development and showed that initial development was accompanied by suberin and lignin deposition, which was followed by polysaccharide deposition characteristic of secondary cell wall formation. These results were compared with those obtained from bamboo. Transmission microspectroscopy was also used to study the infection of potato tubers by Erwinia carotovora ssp. carotovora in aerobic and anaerobic conditions. The spectra suggested that both infective conditions produced cell wall degradation, whereas anaerobic infection was accompanied by extensive breakdown of starch and plasma membrane.

FR-IR Molecular Microanalysis System

1990 ◽  
Vol 48 (2) ◽  
pp. 270-271
Author(s):  
John A. Reffner ◽  
William T. Wihlborg
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Integrated System ◽  
Morphological Examination ◽  
Fully Integrated ◽  
Ir Microscopy ◽  
Normal Functions ◽  
Infrared Microspectroscopy ◽  
Infrared Spectral ◽  
Ft Ir

The IRμs™ is the first fully integrated system for Fourier transform infrared (FT-IR) microscopy. FT-IR microscopy combines light microscopy for morphological examination with infrared spectroscopy for chemical identification of microscopic samples or domains. Because the IRμs system is a new tool for molecular microanalysis, its optical, mechanical and system design are described to illustrate the state of development of molecular microanalysis. Applications of infrared microspectroscopy are reviewed by Messerschmidt and Harthcock.Infrared spectral analysis of microscopic samples is not a new idea, it dates back to 1949, with the first commercial instrument being offered by Perkin-Elmer Co. Inc. in 1953. These early efforts showed promise but failed the test of practically. It was not until the advances in computer science were applied did infrared microspectroscopy emerge as a useful technique. Microscopes designed as accessories for Fourier transform infrared spectrometers have been commercially available since 1983. These accessory microscopes provide the best means for analytical spectroscopists to analyze microscopic samples, while not interfering with the FT-IR spectrometer’s normal functions.

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