Synthesis of dabsylhydrazine and its use in the chromatographic determination of monosaccharides by thin-layer and high-performance liquid chromatography

1987 ◽  
Vol 59 (9) ◽  
pp. 1320-1326 ◽  
Author(s):  
Jen Kun. Lin ◽  
Shan Shou. Wu
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
High Performance ◽  
Chromatographic Determination

Application of thin layer and high performance liquid chromatography to the separation and determination of cephalexin in cephradine, in bulk powder and in pharmaceuticals

1978 ◽  
Vol 11 (11) ◽  
pp. 653-657 ◽  
Author(s):  
E. Crombez ◽  
G. A. Bens ◽  
G. Van der Weken ◽  
W. Van den Bossche ◽  
P. De Moerloose
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
High Performance ◽  
Bulk Powder

Quantitative Determination of Vitamin E Isomers using Gas Chromatography, High Performance Liquid Chromatography and High Performance Thin Layer Chromatography

2017 ◽  
Vol 54 (3) ◽  
pp. 294
Author(s):  
Hari Ramakrishnan K. ◽  
Janaky Ranjithkumar
Keyword(s):  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin E ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Highly Sensitive ◽  
Layer Chromatography

Vitamin E, the fat soluble vitamin is present naturally in some foods and added in food supplements, nutraceuticals etc due to its vital biological function as an antioxidant. Various methods are available for the analysis of vitamin E. Especially High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) are exclusively used for the quantitative evaluation of vitamin E, which has also identified the four different isomeric forms of this vitamin. The rate of losses of this vitamin during food processing and analysis, in addition to their transient dynamics, presents complexities in developing a highly sensitive procedure for their separations. Though effective, HPLC instrument is expensive and comparatively cumbersome. In this prospective, the study was to evaluate the usefulness of High Performance Thin Layer Chromatography (HPTLC) in the analysis of vitamin E. There are methods available using Thin Layer Chromatography for its analysis, but they are not sensitive enough to identify the isomeric forms of vitamin E. In this HPTLC method, the different isomeric forms of vitamin E - α, β, γ and δ were identified. This technique shall be considered as an alternative to the other methods such as HPLC and GC.

Liquid-chromatographic determination of 4-hydroxyproline in urine.

1986 ◽  
Vol 32 (6) ◽  
pp. 1002-1004 ◽  
Author(s):  
H Hughes ◽  
L Hagen ◽  
R A Sutton
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Present Method ◽  
High Performance ◽  
Protein Hydrolysate ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract In this method for 4-hydroxyproline in urine, hydroxyproline is derivatized with 4-chloro-7-nitrobenzofurazan, with subsequent estimation by reversed-phase "high-performance" liquid chromatography. The ranges for excretion of free and total hydroxyproline while the subjects were ingesting unrestricted diets were 2-29 and 122-374 mumol/24 h (n = 21), respectively, with no significant sex-related difference. A comparison with results by colorimetry indicated no significant differences: mean (n = 18) concentrations (mumol/L) of hydroxyproline in urine were 180 (SD 149) by the present method, 163 (SD 166) by colorimetry. For protein hydrolysate the respective values were 5.9 (SD 2.7) and 6.7 (SD 2.9).

Simultaneous Liquid Chromatographic Determination of Thiabendazole, o-Phenylphenol, and Diphenyl Residues in Citrus Fruits, Without Prior Cleanup

1982 ◽  
Vol 65 (6) ◽  
pp. 1302-1304
Author(s):  
Yoshimi Kitada ◽  
Michiko Sasaki ◽  
Kaoru Tanigawa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Citrus Fruits ◽  
Ultraviolet Detection ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A simple, rapid, efficient method has been developed for determining thiabendazole, o-phenylphenol, and diphenyl in citrus fruits by using high performance liquid chromatography, with fluorescence or ultraviolet detection. The compounds are extracted with ethyl acetate and separated from soluble fruit components on a LiChrosorb RP-8 column. Recovery of these compounds added to citrus fruits at 5 or 50 ppm levels was >93%; the limit of detection for the compounds is 1 ppm.

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