Determination of human serum lactate dehydrogenase isoenzymes by anion exchange chromatography

1983 ◽  
Vol 55 (8) ◽  
pp. 1385-1390 ◽  
Author(s):  
M. P. Menon ◽  
Geraldine. Anderson ◽  
G. K. Nambiar
Keyword(s):  
Lactate Dehydrogenase ◽  
Human Serum ◽  
Anion Exchange ◽  
Anion Exchange Chromatography ◽  
Serum Lactate ◽  
Exchange Chromatography ◽  
Serum Lactate Dehydrogenase ◽  
Lactate Dehydrogenase Isoenzymes

A Rapid Electrophoretic Method for the Determination of the Isoenzymes of Serum Lactate Dehydrogenase

1966 ◽  
Vol 12 (5) ◽  
pp. 308-313 ◽  
Author(s):  
Albert W Opher ◽  
Charles S Collier ◽  
Joseph M Miller
Keyword(s):  
Enzyme Activity ◽  
Lactate Dehydrogenase ◽  
Quantitative Determination ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Electrophoretic Method ◽  
The Individual ◽  
Electrophoretic Procedure ◽  
Purple Formazan

Abstract A convenient electrophoretic procedure for the separation and quantitation of lactate dehydrogenase (LDH) isoenzymes is described. The system uses polyacetate Sepraphore III strips.* The areas of activity are shown by incubation with an LDH substrate combined with tetra-nitro-blue-tetrazolium. The reduction of the latter to the purple formazan is quantitatively related to the enzyme activity. Quantitative determination of the individual colored areas is performed by densitometry.

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