A Reactive Poly(ethylene glycol) Layer To Achieve Specific Surface Plasmon Resonance Sensing with a High S/N Ratio:  The Substantial Role of a Short Underbrushed PEG Layer in Minimizing Nonspecific Adsorption

2005 ◽  
Vol 77 (4) ◽  
pp. 1075-1080 ◽  
Author(s):  
Katsumi Uchida ◽  
Hidenori Otsuka ◽  
Mitsuhiro Kaneko ◽  
Kazunori Kataoka ◽  
Yukio Nagasaki





The Analyst ◽  
2014 ◽  
Vol 139 (16) ◽  
pp. 3977-3985 ◽  
Author(s):  
Thangavel Lakshmipriya ◽  
Yukichi Horiguchi ◽  
Yukio Nagasaki

In order to detect a low amount of human coagulation factor IX (FIX), poly(ethylene glycol) (PEG)/aptamer co-immobilized surface was constructed using original PEG–polyamine on surface plasmon resonance (SPR) sensor chip.





2014 ◽  
Vol 10 ◽  
pp. 1672-1680 ◽  
Author(s):  
Silvia Bernardi ◽  
Paola Fezzardi ◽  
Gabriele Rispoli ◽  
Stefania E Sestito ◽  
Francesco Peri ◽  
...  

Four novel calix[4]arene-based glycoclusters were synthesized by conjugating the saccharide units to the macrocyclic scaffold using the CuAAC reaction and using long and hydrophilic ethylene glycol spacers. Initially, two galactosylcalix[4]arenes were prepared starting from saccharide units and calixarene cores which differ in the relative dispositions of the alkyne and azido groups. Once the most convenient synthetic pathway was selected, two further lactosylcalix[4]arenes were obtained, one in the cone, the other one in the 1,3-alternate structure. Preliminary studies of the interactions of these novel glycocalixarenes with galectin-3 were carried out by using a lectin-functionalized chip and surface plasmon resonance. These studies indicate a higher affinity of lactosyl- over galactosylcalixarenes. Furthermore, we confirmed that in case of this specific lectin binding the presentation of lactose units on a cone calixarene is highly preferred with respect to its isomeric form in the 1,3-alternate structure.



RSC Advances ◽  
2015 ◽  
Vol 5 (71) ◽  
pp. 57678-57685 ◽  
Author(s):  
Zhi Wei Low ◽  
Pei Lin Chee ◽  
Dan Kai ◽  
Xian Jun Loh

Hybrid hydrogels, with an elastic modulus and compressive toughness of 350 kPa and 70 J m−3, was synthesized and reported here.



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