Increasing Surface Capacity for On-Probe Affinity Capture MALDI-MS via Gold Particle Attachment to Allyl Amine Plasma Polymers

2011 ◽  
Vol 83 (7) ◽  
pp. 2500-2504 ◽  
Author(s):  
Zaneer M. Segu ◽  
Richard B. Timmons ◽  
Gary R. Kinsel
Keyword(s):  
Gold Particle ◽  
Maldi Ms ◽  
Plasma Polymers ◽  
Affinity Capture ◽  
Probe Affinity ◽  
Allyl Amine ◽  
Particle Attachment

Analysis for TNF-α Using Solid-Phase Affinity Capture with Radiolabel and MALDI-MS Detection

1999 ◽  
Vol 71 (20) ◽  
pp. 4727-4733 ◽  
Author(s):  
Gregory B. Hurst ◽  
Michelle V. Buchanan ◽  
Linda J. Foote ◽  
Stephen J. Kennel
Keyword(s):  
Solid Phase ◽  
Maldi Ms ◽  
Affinity Capture ◽  
Ms Detection ◽  
Tnf Α

Affinity Capture Using Vancomycin-Bound Magnetic Nanoparticles for the MALDI-MS Analysis of Bacteria

2005 ◽  
Vol 77 (6) ◽  
pp. 1753-1760 ◽  
Author(s):  
Ya-Shiuan Lin ◽  
Pei-Jane Tsai ◽  
Mao-Feng Weng ◽  
Yu-Chie Chen
Keyword(s):  
Magnetic Nanoparticles ◽  
Maldi Ms ◽  
Affinity Capture ◽  
Ms Analysis

Identification of Pseudomonas aeruginosa using functional magnetic nanoparticle-based affinity capture combined with MALDI MS analysis

The Analyst ◽  
2009 ◽  
Vol 134 (10) ◽  
pp. 2087 ◽  
Author(s):  
Jr-Chi Liu ◽  
Wei-Jen Chen ◽  
Chen-Wei Li ◽  
Kwok-Kong Tony Mong ◽  
Pei-Jane Tsai ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Magnetic Nanoparticle ◽  
Maldi Ms ◽  
Affinity Capture ◽  
Ms Analysis

scholarly journals Affinity-Bead Assisted Mass Spectrometry (Affi-BAMS): A Multiplexed Microarray Platform for Targeted Proteomics

2020 ◽  
Vol 21 (6) ◽  
pp. 2016
Author(s):  
Ghaith M. Hamza ◽  
Vladislav B. Bergo ◽  
Sergey Mamaev ◽  
Don M. Wojchowski ◽  
Paul Toran ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Binding Capacity ◽  
Microarray Platform ◽  
Magnetic Bead ◽  
Maldi Ms ◽  
Ionization Mass ◽  
Affinity Capture ◽  
Intact Proteins ◽  
Single Bead ◽  
Multiple Samples

The ability to quantitatively probe diverse panels of proteins and their post-translational modifications (PTMs) across multiple samples would aid a broad spectrum of biological, biochemical and pharmacological studies. We report a novel, microarray analytical technology that combines immuno-affinity capture with Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS), which is capable of supporting highly multiplexed, targeted proteomic assays. Termed “Affinity-Bead Assisted Mass Spectrometry” (Affi-BAMS), this LC-free technology enables development of highly specific and customizable assay panels for simultaneous profiling of multiple proteins and PTMs. While affinity beads have been used previously in combination with MS, the Affi-BAMS workflow uses enrichment on a single bead that contains one type of antibody, generally capturing a single analyte (protein or PTM) while having enough binding capacity to enable quantification within approximately 3 orders of magnitude. The multiplexing capability is achieved by combining Affi-BAMS beads with different protein specificities. To enable screening of bead-captured analytes by MS, we further developed a novel method of performing spatially localized elution of targets from individual beads arrayed on a microscope slide. The resulting arrays of micro spots contain highly concentrated analytes localized within 0.5 mm diameter spots that can be directly measured using MALDI MS. While both intact proteins and protein fragments can be monitored by Affi-BAMS, we initially focused on applying this technology for bottom-up proteomics to enable screening of hundreds of samples per day by combining the robust magnetic bead-based workflow with the high throughput nature of MALDI MS acquisition. To demonstrate the variety of applications and robustness of Affi-BAMS, several studies are presented that focus on the response of 4EBP1, RPS6, ERK1/ERK2, mTOR, Histone H3 and C-MET to stimuli including rapamycin, H2O2, EPO, SU11274, Staurosporine and Vorinostat.

Lectin-Based Affinity Capture for MALDI-MS Analysis of Bacteria

1999 ◽  
Vol 71 (7) ◽  
pp. 1460-1463 ◽  
Author(s):  
Jonathan Bundy ◽  
Catherine Fenselau
Keyword(s):  
Maldi Ms ◽  
Affinity Capture ◽  
Ms Analysis

The Shadow Widths of Very Small Particles are Formed Independently of the Metal Cap Build Up on the Particle

1981 ◽  
Vol 39 ◽  
pp. 568-569
Author(s):  
George C. Ruben
Keyword(s):  
Gold Particle ◽  
Film Surface ◽  
Small Particles ◽  
Geometric Process ◽  
Metal Free ◽  
Shadow Area

The formation of shadows behind small particles has been thought to be a geometric process (GP) where the metal cap build up on the particle creates a shadow width the same size as or larger than the particle. This GP cannot explain why gold particle shadow widths are generally larger than the gold particle and may have no appreciable metal cap build up (fig. 1). Ruben and Telford have suggested that particle shadow widths are formed by the width dependent deflection of shadow metal (SM) lateral to and infront of the particle. The trajectory of the deflected SM is determined by the incoming shadow angle (45°). Since there can be up to 1.4 times (at 45°) more SM directly striking the particle than the film surface, a ridge of metal nuclei lateral to and infront of the particle can be formed. This ridge in turn can prevent some SM from directly landing in the metal free shadow area. However, the SM that does land in the shadow area (not blocked by the particle or its ridge) does not stick and apparently surface migrates into the SM film behind the particle.

Characteristic MALDI-MS lipid profiles of Gir, Holstein and crossbred (Gir x Holstein) oocytes recovered by ovum pick-up

2021 ◽  
Vol 243 ◽  
pp. 104380
Author(s):  
Thamiris Vieira Marsico ◽  
José Nélio de Sousa Sales ◽  
Christina Ramires Ferreira ◽  
Mateus José Sudano ◽  
João Henrique Moreira Viana ◽  
...  
Keyword(s):  
Lipid Profiles ◽  
Maldi Ms

Matrix-assisted laser desorption ionisation (MALDI) mass spectrometry (MS): basics and clinical applications

2020 ◽  
Vol 58 (6) ◽  
pp. 883-896 ◽  
Author(s):  
Muhammad Zubair Israr ◽  
Dennis Bernieh ◽  
Andrea Salzano ◽  
Shabana Cassambai ◽  
Yoshiyuki Yazaki ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Maldi Mass Spectrometry ◽  
Clinical Applications ◽  
Clinical Diagnostics ◽  
Maldi Ms ◽  
Therapeutic Drug ◽  
The Impact ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

AbstractBackgroundMatrix-assisted laser desorption ionisation (MALDI) mass spectrometry (MS) has been used for more than 30 years. Compared with other analytical techniques, it offers ease of use, high throughput, robustness, cost-effectiveness, rapid analysis and sensitivity. As advantages, current clinical techniques (e.g. immunoassays) are unable to directly measure the biomarker; rather, they measure secondary signals. MALDI-MS has been extensively researched for clinical applications, and it is set for a breakthrough as a routine tool for clinical diagnostics.ContentThis review reports on the principles of MALDI-MS and discusses current clinical applications and the future clinical prospects for MALDI-MS. Furthermore, the review assesses the limitations currently experienced in clinical assays, the advantages and the impact of MALDI-MS to transform clinical laboratories.SummaryMALDI-MS is widely used in clinical microbiology for the screening of microbial isolates; however, there is scope to apply MALDI-MS in the diagnosis, prognosis, therapeutic drug monitoring and biopsy imaging in many diseases.OutlookThere is considerable potential for MALDI-MS in clinic as a tool for screening, profiling and imaging because of its high sensitivity and specificity over alternative techniques.

scholarly journals Laser Superficial Fusion of Gold Nanoparticles with PEEK Polymer for Cardiovascular Application

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 971
Author(s):  
Oktawian Bialas ◽  
Mateusz Lis ◽  
Anna Woźniak ◽  
Marcin Adamiak
Keyword(s):  
Particle Size ◽  
Laser Beam ◽  
Gold Particle ◽  
Laser Power ◽  
Biomedical Application ◽  
Parameters Optimization ◽  
Size Reduction ◽  
Particle Size Reduction ◽  
Nano Gold ◽  
Gold Particle Size

This paper analyses the possibility of obtaining surface-infused nano gold particles with the polyether ether ketone (PEEK) using picosecond laser treatment. To fuse particles into polymer, the raw surface of PEEK was sputtered with 99.99% Au and micromachined by an A-355 laser device for gold particle size reduction. Biomimetic pattern and parameters optimization were key properties of the design for biomedical application. The structures were investigated by employing surface topography in the presence of micron and sub-micron features. The energy of the laser beam stating the presence of polymer bond thermalisation with remelting due to high temperature was also taken into the account. The process was suited to avoid intensive surface modification that could compromise the mechanical properties of fragile cardiovascular devices. The initial material analysis was conducted by power–depth dependence using confocal microscopy. The evaluation of gold particle size reduction was performed with scanning electron microscopy (SEM), secondary electron (SE) and quadrant backscatter electron detector (QBSD) and energy dispersive spectroscopy (EDS) analysis. The visibility of the constituted coating was checked by a commercial grade X-ray that is commonly used in hospitals. Attempts to reduce deposited gold coating to the size of Au nanoparticles (Au NPs) and to fuse them into the groove using a laser beam have been successfully completed. The relationship between the laser power and the characteristics of the particles remaining in the laser irradiation area has been established. A significant increase in quantity was achieved using laser power with a minimum power of 15 mW. The obtained results allowed for the continuation of the pilot study for augmented research and material properties analysis.

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