4-Methoxy-α-Naphthol as a Spectrophotometric Reagent Substrate for Measuring Peroxidatic Activity.

1964 ◽  
Vol 36 (13) ◽  
pp. 2494-2496 ◽  
Author(s):  
G. G. Guilbault ◽  
D. N. Kramer
Keyword(s):  
Peroxidatic Activity

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

Biogenesis of Catalase-Positive Rods in Excretory Duct Cells of Salivary Glands

1976 ◽  
Vol 34 ◽  
pp. 62-63
Author(s):  
Dwight K. Romanovicz ◽  
Jacob S. Hanker
Keyword(s):  
Light Microscopy ◽  
Salivary Glands ◽  
Submandibular Gland ◽  
Excretory Duct ◽  
Exocrine Glands ◽  
Duct Cells ◽  
Peroxidatic Activity ◽  
Microscopic Studies ◽  
Different Dimensions

The presence of catalase-positive rods (Fig. 1) of different dimensions, which frequently have a crystalline appearance by light microscopy, has been reported. They seem to be related to peroxisomes which were characterized morphologically and cytochemically in parotid and other exocrine glands of the rat by Hand in 1973. Our light microscopic studies of these spherical microbodies and rods of different sizes, stained by virtue of the peroxidatic activity of their catalase, indicate that they are almost entirely confined to the cells of the striated and execretory ducts of the submandibular gland in the mouse. The rods were usually noted only in the proximity of the ductal microbodies. The latter frequently showed a tendency to appear in linear close array, or even to be contiguous (Fig. 2). This suggested that the rods could be formed by the fusion of microbodies.

Peroxidatic activity in monocytes and tissue macrophages of mice

1978 ◽  
Vol 188 (3) ◽  
Author(s):  
T. Ogawa ◽  
H.K. Koerten ◽  
W.Th. Daems
Keyword(s):  
Peroxidatic Activity

scholarly journals CYTOCHEMICAL LOCALIZATION OF PEROXIDASE ACTIVITY IN SACCHAROMYCES CEREVISIAE

1972 ◽  
Vol 20 (5) ◽  
pp. 344-349 ◽  
Author(s):  
MICHAEL M. TODD ◽  
EUGENE L. VIGIL
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cytochrome Oxidase ◽  
Peroxidase Activity ◽  
Rapid Evolution ◽  
Conclusive Evidence ◽  
Yeast Mitochondria ◽  
Yeast Saccharomyces Cerevisiae ◽  
Cytochemical Localization ◽  
Peroxidatic Activity ◽  
High Peroxide

Cytochemical peroxidase activity in a haploid yeast ( Saccharomyces cerevisiae, strain D243) was studied in glutaraldehyde-fixed cells with a diaminobenzidine (DAB) procedure. In early stationary phase cells the predominant sites of peroxidatic activity were the mitochondrial cristae, with activity of lesser intensity on other mitochondrial membranes. The reaction required H2O2, was insensitive to aminotriazole but was blocked by cyanide and azide, by high peroxide concentration or by a decrease in medium pH from 8.5 to 6.0. The enzyme visualized was probably cytochrome c peroxidase, an enzyme known to be highly active in yeast mitochondria, although the possibility that H2O2 may somehow activate DAB oxidation via other mitochondrial heme proteins cannot be completely eliminated. The value of the DAB procedure as a means of specifically staining yeast mitochondria is discussed in relation to studies of mitochondrial biogenesis. No conclusive evidence of the presence of nonmitochondrial peroxisomal organelles (microbodies) was seen in cells grown on either glucose or galactose, but there was an indication of their presence in very low numbers in glycerol-grown yeast. Although catalase was clearly present, as evidenced by the rapid evolution of O2 from the H2O2-containing reaction media, it showed little apparent localized peroxidatic activity toward DAB. We were unable to demonstrate cytochrome oxidase activity in these cells with the usual DAB-cytochrome oxidase procedure.

Development of Exudate-Resident Macrophages, on the Basis of the Pattern of Peroxidatic Activity in Vivo and in Vitro

1980 ◽  
pp. 87-118 ◽  
Author(s):  
R. H. J. Beelen ◽  
Donna M. Fluitsma ◽  
J. W. M. van der Meer ◽  
Elisabeth C. M. Hoefsmit ◽  
Dorothy F. Bainton
Keyword(s):  
Peroxidatic Activity

scholarly journals The development of the resident pattern of endogenous peroxidatic activity in mouse peritoneal macrophages coincides with the expression of the differentiation antigen F4/80. A combined method for immunoperoxidase labeling and endogenous peroxidase cytochemistry.

1986 ◽  
Vol 34 (5) ◽  
pp. 633-640 ◽  
Author(s):  
E C Hoefsmit ◽  
I L Schadee-Eestermans ◽  
R H Beelen
Keyword(s):  
Calf Serum ◽  
Peritoneal Macrophages ◽  
Mononuclear Phagocytes ◽  
Combined Method ◽  
Differentiation Antigen ◽  
Time Points ◽  
Peroxidatic Activity ◽  
Endogenous Peroxidase ◽  
Peroxidase Cytochemistry ◽  
Immunoperoxidase Labeling

In the mouse the maturation of mononuclear phagocytes was followed by comparing the ultrastructural pattern of endogenous peroxidatic activity (PA) at different time points during an acute peritonitis induced with newborn calf serum (NCS). Exudate macrophages demonstrate PA only in lysosomes, whereas resident macrophages have reaction product in the nuclear envelope (NE) and rough endoplasmic reticulum (RER). Transitional cells called "exudate-resident" macrophages have PA in the NE, RER, and some virginal lysosomes. In addition, peroxidase-negative macrophages were also present. A monoclonal antibody, F4/80, that specifically recognizes a mouse macrophage differentiation antigen (Austyn and Gordon, 1981) was used in this study. To compare the indirect immunoperoxidase labeling of this antigen and the endogenous peroxidase cytochemistry on the cellular level, a combined method was developed. Finally, the method was applied to the peritoneal cells at different time points after intraperitoneal injection of NCS in mice. The relative numbers of cells demonstrating the different patterns of endogenous PA and the proportions of each subpopulation expressing F4/80 antigen were estimated. It appeared that the expression of the antigen F4/80 coincides with the development of the resident pattern of PA. It is therefore concluded that the macrophages with the resident pattern of endogenous peroxidase are derived from monocyte-like exudate macrophages. In addition, the results indicate that both exudate-resident macrophages and at least a part of the peroxidase-negative macrophages are transitional forms.

Sign in / Sign up

Export Citation Format

Share Document