Biogenesis of Catalase-Positive Rods in Excretory Duct Cells of Salivary Glands

1976 ◽  
Vol 34 ◽  
pp. 62-63
Author(s):  
Dwight K. Romanovicz ◽  
Jacob S. Hanker
Keyword(s):  
Light Microscopy ◽  
Salivary Glands ◽  
Submandibular Gland ◽  
Excretory Duct ◽  
Exocrine Glands ◽  
Duct Cells ◽  
Peroxidatic Activity ◽  
Microscopic Studies ◽  
Different Dimensions

The presence of catalase-positive rods (Fig. 1) of different dimensions, which frequently have a crystalline appearance by light microscopy, has been reported. They seem to be related to peroxisomes which were characterized morphologically and cytochemically in parotid and other exocrine glands of the rat by Hand in 1973. Our light microscopic studies of these spherical microbodies and rods of different sizes, stained by virtue of the peroxidatic activity of their catalase, indicate that they are almost entirely confined to the cells of the striated and execretory ducts of the submandibular gland in the mouse. The rods were usually noted only in the proximity of the ductal microbodies. The latter frequently showed a tendency to appear in linear close array, or even to be contiguous (Fig. 2). This suggested that the rods could be formed by the fusion of microbodies.

scholarly journals Autoradiographic localization of dihydrotestosterone binding in the major salivary glands and other androgen-responsive organs of the mouse.

1987 ◽  
Vol 35 (10) ◽  
pp. 1053-1058 ◽  
Author(s):  
J I Morrell ◽  
E W Gresik ◽  
T Barka
Keyword(s):  
Growth Factor ◽  
Salivary Glands ◽  
Submandibular Gland ◽  
Cellular Localization ◽  
Incidental Finding ◽  
Acinar Cells ◽  
Unexpected Finding ◽  
Cervical Lymph Nodes ◽  
Major Salivary Glands ◽  
Duct Cells

Mouse submandibular glands show an androgen-dependent sexual dimorphism, reflected in higher concentrations in males than in females of bioactive peptides, such as epidermal growth factor (EGF), nerve growth factor, and renin in the cells of the granular convoluted tubules (GCT). Biochemical studies have demonstrated androgen receptors in submandibular gland and other androgen-responsive organs in mouse. We have determined the cellular localization of these receptors using steroid autoradiography. Fifteen adult gonadectomized male mice were injected intravenously with 0.13 microgram or 0.26 microgram [3H]-dihydrotestosterone (SA 135 Ci/mM); some animals were pre-treated with cyclocytidine to stimulate secretion by GCT cells. Animals were killed 15 min, 1, 2, or 3 hr after isotope injection. Steroid autoradiographs were prepared, and some were stained immunocytochemically for EGF. Of the different cell types of submandibular gland, the acinar cells most frequently and intensely concentrated [3H]-DHT; GCT cells also concentrated the hormone, as did a small number of striated duct cells. In the other major salivary glands, the only cells that concentrated the androgen were interlobular striated duct cells in sublingual gland. In prostate, anterior pituitary, and brain a large number of cells concentrated androgen, as has been previously reported. Androgen binding by the GCT cells was a predictable finding, since androgen-induced alterations in composition and form of these cells are well documented. The intense androgen concentration by the acinar cells was an unexpected finding and suggests a hitherto unknown androgen regulation of these cells. An incidental finding was intense concentration of [3H]-DHT in the nuclei of the endothelial cells of the post-capillary venules of the cervical lymph nodes.

scholarly journals TO THE ULTRASTRUCTURAL ORGANIZATION OF EPITHELIOCYTES OF EXOCRINE GLANDS

2021 ◽  
Vol 245 (1) ◽  
pp. 108-112
Author(s):  
N.V. Momot ◽  
◽  
Y.A. Kolina ◽  
I.L. Kamliya ◽  
◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Salivary Glands ◽  
Standard Technique ◽  
Ultrastructural Organization ◽  
Exocrine Glands ◽  
Electron Microscopic ◽  
Large White ◽  
Microscopic Studies ◽  
Sexually Mature ◽  
Large White Pig

Pieces of the sublingual multi-duct salivary glands of a domestic sexually mature large white pig were fixed in a 4 % paraform solution in 0.1 M phosphate buffer (pH = 7.4) with addi-tional fixation in a 1 % OsO4 solution, dehydrated in alcohols of increasing concentration. Taking into account the recommendations of G. Gayer, pieces of organs were poured into araldite accord-ing to the standard technique. Contrasting was performed according to Reynolds. In the cytoplasm of the mucocytes of the sublingual multi-duct salivary gland, the agranu-lar endoplasmic reticulum predominates, which gives oxyphilic staining. According to electron microscopic studies of mucous glandulocytes in the acini of the sub-lingual salivary glands of domestic pigs, the secretory vacuoles of the cytoplasm are large, with pro-nounced electron-dense membranes. The content of vacuoles in mucocytes of one acinus is differ-ent.

scholarly journals Homeobox Protein, Hmx3, in Postnatally Developing Rat Submandibular Glands

2003 ◽  
Vol 51 (3) ◽  
pp. 385-396 ◽  
Author(s):  
Phyllis A. Shaw ◽  
Xu Zhang ◽  
Andrew F. Russo ◽  
Brad A. Amendt ◽  
Scott Henderson ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Submandibular Gland ◽  
Hox Genes ◽  
Progressive Development ◽  
New Class ◽  
Duct Cells ◽  
Homeobox Protein ◽  
A Cell ◽  
The Central Nervous System ◽  
Cell Type Specific

Homeobox-containing (Hox) genes play important roles in development, particularly in the development of neurons and sensory organs, and in specification of body plan. The Hmx gene family is a new class of homeobox-containing genes defined by a conserved homeobox region and a characteristic pattern of expression in the central nervous system that is more rostral than that of the Hox genes. To date, three closely related members of the Hmx family, Hmx1, Hmx2, and Hmx3, have been described. All three Hmx genes are expressed in the craniofacial region of developing embryos. Here we show, for the first time, the expression of the transcription factor Hmx3 in postnatally developing salivary glands. Hmx3 protein is expressed in a cell type-specific manner in rat salivary glands. Hmx3 is present in both the nuclei and cytoplasm of specific groups of duct cells of the submandibular, parotid, and sublingual glands. Hmx3 expression increases during postnatal development of the submandibular gland. The duct cells show increasing concentrations of Hmx3 protein with progressive development of the submandibular gland. In contrast, the acinar cells of the three salivary glands do not exhibit detectable levels of Hmx3 protein.

Induction of Sca-1 via activation of STAT3 system in the duct cells of the mouse submandibular gland by ligation of the main excretory duct

2011 ◽  
Vol 301 (5) ◽  
pp. G814-G824 ◽  
Author(s):  
Nunuk Purwanti ◽  
Mileva Ratko Karabasil ◽  
Shinsuke Matsuo ◽  
Gang Chen ◽  
Purevjav Javkhlan ◽  
...  
Keyword(s):  
Submandibular Gland ◽  
Tissue Injury ◽  
Specific Binding ◽  
Initial Step ◽  
Nuclear Extract ◽  
Excretory Duct ◽  
Protein Marker ◽  
Hematopoietic Stem ◽  
Duct Cells ◽  
Stat3 Phosphorylation

To examine the very initial step that takes place immediately after tissue injury and is linked to tissue regeneration, we employed the submandibular gland (SMG), which was injured by ligation of its main excretory duct (MED). Ligation of the MED of the SMG in mice induced the expression of Sca-1, a protein marker of hematopoietic stem cells. In the normal gland, a low level of Sca-1 was expressed, which was localized predominantly in the excretory duct cells. At 1 day after ligation, Sca-1 expression increased prominently in almost all of cells in the duct system, but not in the acinar cells. The level of Sca-1 mRNA had begun to increase at 6 h after ligation and continuously rose thereafter until it reached a plateau, which occurred ∼12 h after ligation. STAT3 phosphorylated at its tyrosine-705 (p-STAT3) in the ligated gland increased immediately after ligation, and it was localized in the nuclei of all duct cells. The results of an EMSA revealed the specific binding of a nuclear extract to the sequence of the γ-interferon activation site (GAS) present in the Sca-1 promoter and confirmed that such binding increased after ligation. Thus the present study suggests that STAT3, having been phosphorylated following MED ligation, was transferred to the nucleus, where it bound to the GAS element in the promoter of Sca-1 gene, resulting in promotion of Sca-1 gene expression. Actual prevention of STAT3 phosphorylation reduced the ligation-induced Sca-1 elevation.

scholarly journals Differential localization of "brain-specific" S-100 and its subunits in rat salivary glands.

1984 ◽  
Vol 32 (8) ◽  
pp. 805-814 ◽  
Author(s):  
S O Molin ◽  
L Rosengren ◽  
K Haglid ◽  
J Baudier ◽  
A Hamberger
Keyword(s):  
Salivary Glands ◽  
Submandibular Gland ◽  
Beta Subunit ◽  
Ependymal Cells ◽  
Beta Subunits ◽  
Differential Distribution ◽  
Rat Salivary Glands ◽  
Duct Cells ◽  
S 100

In the rat, the S-100 antigens in the submandibular gland were found to be immunochemically identical with those in the brain (glial cells) when compared using crossed immunoelectrophoresis. Specific antibodies against the S-100a non-beta and against the S-100 beta subunit were prepared from antibodies against crude S-100 protein and from S-100 components (S-100a and b) by affinity chromatography. In the rat salivary glands a differential distribution of subunit immunoreactivity was clearly evidenced using indirect immunofluorescence. Certain intercalated duct cells of the submandibular gland as well as Schwann cells contained the S-100 beta subunit immunoreactivity exclusively, while other duct cells in parotid, submandibular, and sublingual glands contained S-100a non-beta subunit immunoreactivity. Both subunits were present in astrocytes and ependymal cells. The immunocytochemical localization of alpha and beta subunits is a promising technique for the classification of various types of S-100-containing cells.

scholarly journals Immunolocalization of Vacuolar-type H+-ATPase in Rat Submandibular Gland and Adaptive Changes Induced by Acid–Base Disturbances

1998 ◽  
Vol 46 (1) ◽  
pp. 91-100 ◽  
Author(s):  
Eleni Roussa ◽  
Frank Thévenod ◽  
Ivan Sabolic ◽  
Carol M. Herak–Kramberger ◽  
Wolfgang Nastainczyk ◽  
...  
Keyword(s):  
Metabolic Acidosis ◽  
Submandibular Gland ◽  
Excretory Duct ◽  
Acid Base ◽  
Chronic Metabolic Acidosis ◽  
Adaptive Changes ◽  
Duct Cells ◽  
Rat Submandibular Gland ◽  
Diffuse Distribution ◽  
Apical Localization

Using antibodies against the 31-kD and 70-kD subunits of vacuolar type H+-ATPase (V-ATPase) and light microscopic immunocytochemistry, we have demonstrated the presence of this V-ATPase in rat submandibular gland. We have also investigated the adaptive changes of this transporter during acid-base disturbances such as acute and chronic metabolic acidosis or alkalosis. Our results show intracellularly distributed V-ATPase in striated, granular, and main excretory duct cells in controls, but no V-ATPase immunoreaction in acinar cells. Both acute and chronic metabolic acidosis caused a shift in V-ATPase away from diffuse distribution towards apical localization in striated and granular duct cells, suggesting that a V-ATPase could be involved in the regulation of acid–base homeostasis. In contrast, during acidosis the main excretory duct cells showed no changes in the V-ATPase distribution compared to controls. With acute and chronic metabolic alkalosis, no changes in the V-ATPase distribution occurred.

Induction of Sca-1 in the duct cells of the mouse submandibular gland by obstruction of the main excretory duct

2011 ◽  
Vol 40 (8) ◽  
pp. 651-658 ◽  
Author(s):  
Nunuk Purwanti ◽  
Daisuke Tsuji ◽  
Ahmad Azlina ◽  
Mileva Ratko Karabasil ◽  
Purevjav Javkhlan ◽  
...  
Keyword(s):  
Submandibular Gland ◽  
Excretory Duct ◽  
Duct Cells

scholarly journals Electron Microscopic Immunogold Localization of Salivary Mucins MG1 and MG2 in Human Submandibular and Sublingual Glands

2003 ◽  
Vol 51 (1) ◽  
pp. 69-79 ◽  
Author(s):  
Marco Piludu ◽  
Sean A. Rayment ◽  
Bing Liu ◽  
Gwynneth D. Offner ◽  
Frank G. Oppenheim ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Expression Patterns ◽  
Cell Types ◽  
Mucous Cells ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Dense Cores ◽  
Duct Cells ◽  
Rabbit Igg ◽  
Lowicryl K4m

The human salivary mucins MG1 and MG2 are well characterized biochemically and functionally. However, there is disagreement regarding their cellular and glandular sources. The aim of this study was to define the localization and distribution of these two mucins in human salivary glands using a postembedding immunogold labeling method. Normal salivary glands obtained at surgery were fixed in 3% paraformaldehyde-0.1% glutaraldehyde and embedded in Lowicryl K4M or LR Gold resin. Thin sections were labeled with rabbit antibodies to MG1 or to an N-terminal synthetic peptide of MG2, followed by gold-labeled goat anti-rabbit IgG. The granules of all mucous cells of the submandibular and sublingual glands were intensely reactive with anti-MG1. No reaction was detected in serous cells. With anti-MG2, the granules of both mucous and serous cells showed reactivity. The labeling was variable in both cell types, with mucous cells exhibiting a stronger reaction in some glands and serous cells in others. In serous granules, the electron-lucent regions were more reactive than the dense cores. Intercalated duct cells near the acini displayed both MG1 and MG2 reactivity in their apical granules. In addition, the basal and lateral membranes of intercalated duct cells were labeled with anti-MG2. These results confirm those of earlier studies on MG1 localization in mucous cells and suggest that MG2 is produced by both mucous and serous cells. They also indicate differences in protein expression patterns among salivary serous cells.

Three-Dimensional Localization of DNA Synthesis in Secretory Elements of Adult Female Mouse Submandibular Gland

1990 ◽  
Vol 4 (1) ◽  
pp. 34-44 ◽  
Author(s):  
P.C. Denny ◽  
Y. Chai ◽  
D.K. Klauser ◽  
P.A. Denny
Keyword(s):  
Dna Synthesis ◽  
Submandibular Gland ◽  
Adult Female ◽  
Female Mouse ◽  
Three Dimensional ◽  
Acinar Cells ◽  
Single Pulse ◽  
Duct System ◽  
Duct Cells ◽  
Structural Relationships

A system based in part on three-dimensional structural relationships is described for precisely characterizing the location of cells within secretory complexes of the adult female mouse submandibular gland. The pattern of DNA synthesis during a 90-minute pulse with 3H-thymidine was characterized based upon the above system. Seventy-eight percent of all radiolabeled nuclei were found in the intercalated duct system. One-half of these were in second-order intercalated ducts. DNA synthesis was also observed in acinar cells, granular intercalated duct cells, striated granular duct cells, and granular duct cells. Some secretory complexes contained multiple radiolabeled nuclei, with some of these nuclei in a side-by-side configuration. Approximately one-half of all secretory complexes contained radiolabeled nuclei. A second survey of the frequency of complexes containing radiolabeled nuclei was conducted following four pulses at eight-hour intervals over a 26-hour period. Only about 30% of all complexes contained radiolabeled nuclei. This reduction in the frequency of radiolabeled nuclei when compared with the single pulse suggests the possibility of individual variation. However, a more prolonged period of daily injections for nine days with 3H-thymidine resulted in all but one of the secretory complexes containing radiolabeled nuclei. This latter observation suggests that cell addition in adult submandibular glands is widespread.

Synchronous pleomorphic adenoma in the infratemporal fossa and contralateral submandibular gland and endoscopic removal

2021 ◽  
Vol 14 (5) ◽  
pp. e237775
Author(s):  
Shahul Hameed Poondiyar Sirajuddin ◽  
Rayappa Chunnusamy
Keyword(s):  
Salivary Glands ◽  
Submandibular Gland ◽  
Pleomorphic Adenoma ◽  
Nasal Septum ◽  
Infratemporal Fossa ◽  
Endoscopic Removal ◽  
Minor Salivary Glands ◽  
Main Stem Bronchus ◽  
Diagnostic Dilemma ◽  
First Case

The objective of this article is to elucidate on our experience with the first case reported in the literature of a synchronous presentation of pleomorphic adenoma (PA) in infratemporal fossa as well as the contralateral submandibular gland. PA most commonly arises from the major salivary glands but has also been reported to arise from minor salivary glands in unusual sites such as the nasal septum, main stem bronchus, trachea, lacrimal gland, external auditory canal, etc. However, it seldom involves the infratemporal fossa and extremely few cases are reported in the literature and coexistent contralateral synchronous PA is nowhere reported in literature. The infratemporal fossa tumour posed a diagnostic dilemma and therapeutic challenge because of its concealed location which we dealt successfully with endoscopic sublabial trans maxillary approach.

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