Self-Assembled Growing DNA Tree Mediated by Exosomes for Amplified Imaging of Messenger RNA in Living Cells

A formal analogy of fluctuating diffusivity to thermodynamics is discussed for messenger RNA molecules fluorescently fused to a protein in living cells. Regarding the average value of the fluctuating diffusivity of such RNA-protein particles as the analog of the internal energy, the analogs of the quantity of heat and work are identified. The Clausius-like inequality is shown to hold for the entropy associated with diffusivity fluctuations, which plays a role analogous to the thermodynamic entropy, and the analog of the quantity of heat. The change of the statistical fluctuation distribution is also examined from a geometric perspective. The present discussions may contribute to a deeper understanding of the fluctuating diffusivity in view of the laws of thermodynamics.

Download Full-text

Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living Cells

Nature ◽

10.1038/233177a0 ◽

1971 ◽

Vol 233 (5316) ◽

pp. 177-182 ◽

Cited By ~ 486

Author(s):

J. B. GURDON ◽

C. D. LANE ◽

H. R. WOODLAND ◽

G. MARBAIX

Keyword(s):

Messenger Rna ◽

Living Cells

Download Full-text

Messenger RNA Trafficking in Living Cells

Single Particle Tracking and Single Molecule Energy Transfer ◽

10.1002/9783527628360.ch3 ◽

2009 ◽

pp. 43-65 ◽

Cited By ~ 1

Author(s):

Ulrich Kubitscheck ◽

Roman Veith ◽

Jrg Ritter ◽

Jan-Peter Siebrasse

Keyword(s):

Messenger Rna ◽

Living Cells ◽

Rna Trafficking

Download Full-text

A novel self-assembled nanoprobe for the detection of aluminum ions in real water samples and living cells

Talanta ◽

10.1016/j.talanta.2018.09.104 ◽

2019 ◽

Vol 194 ◽

pp. 38-45 ◽

Cited By ~ 16

Author(s):

Yi Liu ◽

Anyao Bi ◽

Tang Gao ◽

Xiaozheng Cao ◽

Feng Gao ◽

...

Keyword(s):

Water Samples ◽

Living Cells ◽

Real Water Samples ◽

Aluminum Ions ◽

Self Assembled

Download Full-text

Numerical operations in living cells by programmable RNA devices

Science Advances ◽

10.1126/sciadv.aax0835 ◽

2019 ◽

Vol 5 (8) ◽

pp. eaax0835 ◽

Cited By ~ 4

Author(s):

Kei Endo ◽

Karin Hayashi ◽

Hirohide Saito

Keyword(s):

Messenger Rna ◽

Design Principle ◽

Logic Gates ◽

Living Cells ◽

Multiple Biomarkers ◽

Intracellular Changes ◽

And Function ◽

Single Living Cells ◽

Single Living ◽

Numerical Operations

Integrated bioengineering systems can make executable decisions according to the cell state. To sense the state, multiple biomarkers are detected and processed via logic gates with synthetic biological devices. However, numerical operations have not been achieved. Here, we show a design principle for messenger RNA (mRNA) devices that recapitulates intracellular information by multivariate calculations in single living cells. On the basis of this principle and the collected profiles of multiple microRNA activities, we demonstrate that rationally programmed mRNA sets classify living human cells and track their change during differentiation. Our mRNA devices automatically perform multivariate calculation and function as a decision-maker in response to dynamic intracellular changes in living cells.

Download Full-text