Modality Agnostic Model for Spatial Resolution in Mass Spectrometry Imaging: Application to MALDI MSI Data

2021 ◽  
Author(s):  
Martin D. Metodiev ◽  
Rory T. Steven ◽  
Xavier Loizeau ◽  
Zoltan Takats ◽  
Josephine Bunch
Keyword(s):  
Mass Spectrometry ◽  
Spatial Resolution ◽  
Mass Spectrometry Imaging ◽  
Imaging Application

scholarly journals NanoSIMS imaging: an approach for visualizing and quantifying lipids in cells and tissues

2016 ◽  
Vol 65 (3) ◽  
pp. 669-672 ◽  
Author(s):  
Cuiwen He ◽  
Loren G Fong ◽  
Stephen G Young ◽  
Haibo Jiang
Keyword(s):  
Mass Spectrometry ◽  
Spatial Resolution ◽  
Mass Spectrometry Imaging ◽  
Secondary Ion Mass Spectrometry ◽  
High Spatial Resolution ◽  
High Sensitivity ◽  
New Approach ◽  
The Past ◽  
Secondary Ion ◽  
In Cells

Over the past few decades, several approaches have been used to image lipids in cells and tissues, but most have limited spatial resolution and sensitivity. Here, we discuss a relatively new approach, nanoscale secondary ion mass spectrometry imaging, that makes it possible to visualize lipids in cells and tissues in a quantitative fashion and with high spatial resolution and high sensitivity.

scholarly journals IR-MALDESI Mass Spectrometry Imaging at 50 Micron Spatial Resolution

2017 ◽  
Vol 28 (10) ◽  
pp. 2099-2107 ◽  
Author(s):  
Mark T. Bokhart ◽  
Jeffrey Manni ◽  
Kenneth P. Garrard ◽  
Måns Ekelöf ◽  
Milad Nazari ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Spatial Resolution ◽  
Mass Spectrometry Imaging

A Simple Method for Improving the Spatial Resolution in Infrared Laser Ablation Mass Spectrometry Imaging

2017 ◽  
Vol 28 (6) ◽  
pp. 1060-1065 ◽  
Author(s):  
Juha-Pekka Hieta ◽  
Anu Vaikkinen ◽  
Samuli Auno ◽  
Heikki Räikkönen ◽  
Markus Haapala ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Spatial Resolution ◽  
Mass Spectrometry Imaging ◽  
Infrared Laser ◽  
Simple Method

Characterisation of a micro-plasma for ambient mass spectrometry imaging

The Analyst ◽  
2014 ◽  
Vol 139 (21) ◽  
pp. 5430-5438 ◽  
Author(s):  
Andrew Bowfield ◽  
Josephine Bunch ◽  
Tara L. Salter ◽  
Rory T. Steven ◽  
Ian S. Gilmore ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Spatial Resolution ◽  
Mass Spectrometry Imaging ◽  
Ion Source ◽  
Ambient Mass Spectrometry

A systematic characterisation and optimisation of parameters of a plasma-mediated ion source to achieve the best spatial resolution for MSI.

scholarly journals Nanospray Desorption Electrospray Ionization (nano-DESI) Mass Spectrometry Imaging of Drift Time-Separated Ions

2020 ◽  
Author(s):  
Daisy Unsihuay ◽  
ruichuan yin ◽  
Daniela Mesa Sanchez ◽  
Yingju Li ◽  
Xiaofei Sun ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Spatial Resolution ◽  
Ion Mobility ◽  
Mass Spectrometry Imaging ◽  
Structural Information ◽  
Biological Systems ◽  
Drift Time ◽  
Desorption Electrospray Ionization ◽  
Chemical Information

Simultaneous spatial localization and structural characterization of molecules in complex biological samples currently represents an analytical challenge for mass spectrometry imaging (MSI) techniques. In this study, we describe a novel experimental platform, which substantially expands the capabilities and enhances the depth of chemical information obtained in high spatial resolution MSI experiments performed using nanospray desorption electrospray ionization (nano-DESI). Specifically, we designed and constructed a portable nano-DESI MSI platform and coupled it with a drift tube ion mobility spectrometer-mass spectrometer (IM-MS). Separation of biomolecules observed in MSI experiments based on their drift times provides unique molecular descriptors necessary for their identification by comparison with databases. Furthermore, it enables isomer-specific imaging, which is particularly important for unraveling the complexity of biological systems. Imaging of day 4 pregnant mouse uterine sections using the newly developed nano-DESI-IM-MSI system demonstrates rapid isobaric and isomeric separation and reduced chemical noise in MSI experiments. A direct comparison of the performance of the new nano-DESI-MSI platform operated in the MS mode with the more established nano-DESI-Orbitrap platform indicates a comparable performance of these two systems. A spatial resolution of better than ~16 µm and similar molecular coverage was obtained using both platforms. The structural information provided by the ion mobility separation expands the molecular specificity of high-resolution MSI necessary for the detailed understanding of biological systems.

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