C80 micro calorimeter was applied to investigate the initial heat release temperature and heat output at low-temperature oxidation process of Kabuliang anthracite coal under the experimental conditions of different coal sample mass, different temperature rising rates and different gas flow, and analyzed the effect of experimental conditions on test results. The results indicate that the coal sample mass and temperature rising rate affect measurement results, while there is no effect of gas flow. Under the same experimental condition, a higher temperature rising rate leads to a higher initial heat release temperature and less output of heat; The initial heat release temperature shows the trend of increase after decrease with the increase of coal sample mass. So, when micro calorimeter is used to investigate the coal low-temperature oxidation process, the experiments under the conditions of appropriate coal sample mass and the lower temperature rising rate can obtain more accurate test parameters.